INSY1_MOUSE
ID INSY1_MOUSE Reviewed; 292 AA.
AC Q8CD60;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Inhibitory synaptic factor 1 {ECO:0000303|PubMed:27609886};
DE Short=InSyn1 {ECO:0000303|PubMed:27609886};
GN Name=Insyn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH GPHN.
RX PubMed=27609886; DOI=10.1126/science.aag0821;
RA Uezu A., Kanak D.J., Bradshaw T.W., Soderblom E.J., Catavero C.M.,
RA Burette A.C., Weinberg R.J., Soderling S.H.;
RT "Identification of an elaborate complex mediating postsynaptic
RT inhibition.";
RL Science 353:1123-1129(2016).
CC -!- FUNCTION: Component of the protein machinery at the inhibitory
CC synapses, probably acting as a scaffold. Inhibitory synapses dampen
CC neuronal activity through postsynaptic hyperpolarization. This synaptic
CC inhibition is fundamental for the functioning of the central nervous
CC system, shaping and orchestrating the flow of information through
CC neuronal networks to generate a precise neural code.
CC {ECO:0000269|PubMed:27609886}.
CC -!- SUBUNIT: Interacts with GPHN. {ECO:0000269|PubMed:27609886}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic density
CC {ECO:0000269|PubMed:27609886}.
CC -!- SIMILARITY: Belongs to the INSYN1 family. {ECO:0000305}.
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DR EMBL; AK031382; BAC27375.1; -; mRNA.
DR EMBL; BC096614; AAH96614.1; -; mRNA.
DR CCDS; CCDS23243.1; -.
DR RefSeq; NP_795895.1; NM_176921.3.
DR AlphaFoldDB; Q8CD60; -.
DR SMR; Q8CD60; -.
DR STRING; 10090.ENSMUSP00000082800; -.
DR PhosphoSitePlus; Q8CD60; -.
DR jPOST; Q8CD60; -.
DR PaxDb; Q8CD60; -.
DR PRIDE; Q8CD60; -.
DR Antibodypedia; 52382; 15 antibodies from 9 providers.
DR DNASU; 319477; -.
DR Ensembl; ENSMUST00000085658; ENSMUSP00000082800; ENSMUSG00000066607.
DR Ensembl; ENSMUST00000216294; ENSMUSP00000148914; ENSMUSG00000066607.
DR GeneID; 319477; -.
DR KEGG; mmu:319477; -.
DR UCSC; uc009pwz.1; mouse.
DR CTD; 388135; -.
DR MGI; MGI:2442108; Insyn1.
DR VEuPathDB; HostDB:ENSMUSG00000066607; -.
DR eggNOG; ENOG502R2SS; Eukaryota.
DR GeneTree; ENSGT00910000144204; -.
DR HOGENOM; CLU_073752_1_0_1; -.
DR InParanoid; Q8CD60; -.
DR OMA; EDKSGCA; -.
DR OrthoDB; 1272531at2759; -.
DR PhylomeDB; Q8CD60; -.
DR TreeFam; TF332382; -.
DR BioGRID-ORCS; 319477; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q8CD60; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8CD60; protein.
DR Bgee; ENSMUSG00000066607; Expressed in striatum and 52 other tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0014069; C:postsynaptic density; IDA:UniProtKB.
DR GO; GO:0060080; P:inhibitory postsynaptic potential; IDA:UniProtKB.
DR InterPro; IPR027997; Largen/INSYN1.
DR PANTHER; PTHR15917; PTHR15917; 1.
DR Pfam; PF15252; DUF4589; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Reference proteome; Synapse.
FT CHAIN 1..292
FT /note="Inhibitory synaptic factor 1"
FT /id="PRO_0000337046"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 30..63
FT /evidence="ECO:0000255"
FT COMPBIAS 182..199
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..217
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 292 AA; 31813 MW; 2904EAF1BC4B6963 CRC64;
MNIRGAPDLG QPSDDPNSGG ERERIRQRMK MVIGQLEGIL RELKEVAKEL REVVSQIDKL
TSDFDFELEP DDWTTATVSS TSSSDKAGVG GPFDLGHLDF MTADILSDSW EFCSFLDVST
PSDSVDGPEA PRPGTGPDYQ LMNGGLPIPN GPRVETPDSS SEEAFSAGPA KGQVPQRTPG
TRERVRFSDK VLYHALCCDD EEGDGEEGEE EEEGDLAPEL PRVEPHTGPL KPSPAPYKTK
RSPLTTRRLG PTLAPEQTRR VTRNSSTQTV SDKSTQTVLP YTATKQKAKG KN
