ID   INSY2_MOUSE             Reviewed;         422 AA.
AC   Q3USH1; B2RY32;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Inhibitory synaptic factor 2A {ECO:0000305};
DE            Short=InSyn2 {ECO:0000303|PubMed:27609886};
GN   Name=Insyn2a {ECO:0000312|MGI:MGI:3605068};
GN   Synonyms=Fam196a {ECO:0000312|MGI:MGI:3605068},
GN   Insy2 {ECO:0000312|MGI:MGI:3605068};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain cortex;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4]
RP   SUBCELLULAR LOCATION, INTERACTION WITH GPHN, AND FUNCTION.
RX   PubMed=27609886; DOI=10.1126/science.aag0821;
RA   Uezu A., Kanak D.J., Bradshaw T.W., Soderblom E.J., Catavero C.M.,
RA   Burette A.C., Weinberg R.J., Soderling S.H.;
RT   "Identification of an elaborate complex mediating postsynaptic
RT   inhibition.";
RL   Science 353:1123-1129(2016).
CC   -!- FUNCTION: Component of the protein machinery at the inhibitory
CC       synapses, probably acting as a scaffold. Inhibitory synapses dampen
CC       neuronal activity through postsynaptic hyperpolarization. This synaptic
CC       inhibition is fundamental for the functioning of the central nervous
CC       system, shaping and orchestrating the flow of information through
CC       neuronal networks to generate a precise neural code.
CC       {ECO:0000269|PubMed:27609886}.
CC   -!- SUBUNIT: Interacts with GPHN. {ECO:0000269|PubMed:27609886}.
CC   -!- SUBCELLULAR LOCATION: Postsynaptic density
CC       {ECO:0000269|PubMed:27609886}.
CC   -!- SIMILARITY: Belongs to the INSYN2 family. {ECO:0000305}.
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DR   EMBL; AK140373; BAE24361.1; -; mRNA.
DR   EMBL; BC158077; AAI58078.1; -; mRNA.
DR   EMBL; BC158080; AAI58081.1; -; mRNA.
DR   AlphaFoldDB; Q3USH1; -.
DR   SMR; Q3USH1; -.
DR   STRING; 10090.ENSMUSP00000129222; -.
DR   iPTMnet; Q3USH1; -.
DR   PhosphoSitePlus; Q3USH1; -.
DR   PaxDb; Q3USH1; -.
DR   PRIDE; Q3USH1; -.
DR   ProteomicsDB; 271829; -.
DR   UCSC; uc009kdz.1; mouse.
DR   MGI; MGI:3605068; Insyn2a.
DR   eggNOG; ENOG502QS3U; Eukaryota.
DR   InParanoid; Q3USH1; -.
DR   PhylomeDB; Q3USH1; -.
DR   PRO; PR:Q3USH1; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q3USH1; protein.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0014069; C:postsynaptic density; IDA:UniProtKB.
DR   GO; GO:0060080; P:inhibitory postsynaptic potential; IDA:UniProtKB.
DR   InterPro; IPR029337; INSYN2.
DR   PANTHER; PTHR28682; PTHR28682; 1.
DR   Pfam; PF15265; FAM196; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Phosphoprotein; Reference proteome; Synapse.
FT   CHAIN           1..422
FT                   /note="Inhibitory synaptic factor 2A"
FT                   /id="PRO_0000329447"
FT   REGION          143..163
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          344..370
FT                   /evidence="ECO:0000255"
FT   MOD_RES         177
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
SQ   SEQUENCE   422 AA;  46222 MW;  65836FDBE3B0774E CRC64;
     MVSKDSGRCI LTTPEREVEP AACLALEMRY ALDPNRQIKK RNKALQVRFK DICEAQNEQR
     DTQLSSGPLG EKREAKAVSC RVAYRKYMTV PARRSIPNVT KSTGVQTSPD LRKCYQTFPL
     DRKKGSLKGL PAADAFKSQN NGFLADSKEK SEAGPMEEPR PCSAGRIHKT TALVFHSNEH
     VNALGQPSGV NCAELCKSPD VLGYPEAVLQ NSRPPSEEPI HQLQGRAKLD RGTLDSEEPA
     PLTHGRVFKT EVATVYPPAI STRAPEPGLS NSAAASQWSL CPADEEQRRA AHLNGLQAST
     GSAVACSTPV QYLSPECSEQ PLQNQPSPTA GIGDEEHQQI VPHTEVVDLK AQLQVMENLI
     SSSQETIKVL LGVIQELEKG EAHREGLSYR TGQDTANCDT CRNSACIIYR YVNILFPLCY
     SE