ID   INS_ONCKE               Reviewed;         105 AA.
AC   P04667; Q91477; Q91478; Q91479;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Insulin;
DE   Contains:
DE     RecName: Full=Insulin B chain;
DE   Contains:
DE     RecName: Full=Insulin A chain;
DE   Flags: Precursor;
GN   Name=ins;
OS   Oncorhynchus keta (Chum salmon) (Salmo keta).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=8018;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Sperm;
RX   PubMed=2757686; DOI=10.1093/nar/17.4.1758;
RA   Koval A.P., Petrenko A.I., Kavsan V.M.;
RT   "Sequence of the salmon (Oncorhynchus keta) preproinsulin gene.";
RL   Nucleic Acids Res. 17:1758-1758(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Sorokin A.V., Skoblov Y.S., Mironov A.A., Zlochevskii M.L.,
RA   Dem'Yanova N.G., Rebentish B.A., Kozlov Y.I., Kavsan V.M., Sova V.V.,
RA   Debabov V.G.;
RT   "Nucleotide sequence of cloned insulin cDNA of Oncorhyncus keta.";
RL   Dokl. Biochem. 269:80-83(1983).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6897724; DOI=10.1016/0378-1119(82)90205-0;
RA   Sorokin A.V., Petrenko A.I., Kavsan V.M., Kozlov Y.I., Debabov V.G.,
RA   Zlochevskii M.L.;
RT   "Nucleotide sequence analysis of the cloned salmon preproinsulin cDNA.";
RL   Gene 20:367-376(1982).
RN   [4]
RP   NUCLEOTIDE SEQUENCE OF 26-51.
RX   AGRICOLA=IND81114320;
RA   Dem'Yanova N.G., Zlochevskii M.L., Kavsan V.M., Kozlov Y.I., Petrenko A.I.,
RA   Polyakova N.E., Prokopenko I.V., Rebentish B.A., Ryndich A.V.,
RA   Skoblov Y.S., Sova V.V., Yurin V.L., Yankovskii N.K., Debabov V.G.;
RT   "Cloning of the insulin gene of a fish (Oncorhynchus keta) in Escherichia
RT   coli.";
RL   Dokl. Biochem. 256:35-38(1981).
RN   [5]
RP   PROTEIN SEQUENCE OF 23-50 AND 85-105.
RX   PubMed=3314270;
RA   Rusakov Y.I., Karasev V.S., Pertseva M.N., Pankov Y.A.;
RT   "Amino acid sequence of the insulin of the dog salmon Oncorhynchus keta.";
RL   Zh. Evol. Biokhim. Fiziol. 23:473-480(1987).
CC   -!- FUNCTION: Insulin decreases blood glucose concentration. It increases
CC       cell permeability to monosaccharides, amino acids and fatty acids. It
CC       accelerates glycolysis, the pentose phosphate cycle, and glycogen
CC       synthesis in liver.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; X00148; CAA24983.1; ALT_SEQ; mRNA.
DR   EMBL; J00936; AAA49414.1; ALT_SEQ; mRNA.
DR   EMBL; K01655; AAA49417.1; -; mRNA.
DR   EMBL; X13559; CAA31910.1; -; Genomic_DNA.
DR   PIR; S02722; IPON.
DR   AlphaFoldDB; P04667; -.
DR   SMR; P04667; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd04367; IlGF_insulin_like; 1.
DR   InterPro; IPR004825; Insulin.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   PANTHER; PTHR11454; PTHR11454; 1.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR00277; INSULIN.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Glucose metabolism; Hormone;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:3314270"
FT   PEPTIDE         23..50
FT                   /note="Insulin B chain"
FT                   /id="PRO_0000015858"
FT   PROPEP          53..82
FT                   /note="C peptide"
FT                   /id="PRO_0000015859"
FT   PEPTIDE         85..105
FT                   /note="Insulin A chain"
FT                   /evidence="ECO:0000269|PubMed:6897724"
FT                   /id="PRO_0000015860"
FT   DISULFID        29..91
FT                   /note="Interchain (between B and A chains)"
FT   DISULFID        41..104
FT                   /note="Interchain (between B and A chains)"
FT   DISULFID        90..95
FT   CONFLICT        27
FT                   /note="Missing (in Ref. 4)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        49
FT                   /note="T -> N (in Ref. 5; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   105 AA;  11714 MW;  A928FDD5CFFDA7A6 CRC64;
     MAFWLQAASL LVLLALSPGV DAAAAQHLCG SHLVDALYLV CGEKGFFYTP KRDVDPLIGF
     LSPKSAKENE EYPFKDQTEM MVKRGIVEQC CHKPCNIFDL QNYCN