INT10_DROME
ID INT10_DROME Reviewed; 631 AA.
AC Q9VZM7;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Integrator complex subunit 10 {ECO:0000303|PubMed:21078872};
GN Name=IntS10 {ECO:0000312|FlyBase:FBgn0035462};
GN ORFNames=CG1120 {ECO:0000312|FlyBase:FBgn0035462};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAV36868.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAV36868.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAV36868.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). May be
CC involved in the 3'-end processing of the spliceosomal snRNAs U1, U2, U4
CC and U5 but is probably not essential for 3'-end processing of the snRNA
CC U7 (PubMed:21078872, PubMed:23097424). {ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 10 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF47793.1; -; Genomic_DNA.
DR EMBL; BT015983; AAV36868.1; -; mRNA.
DR RefSeq; NP_647830.1; NM_139573.2.
DR AlphaFoldDB; Q9VZM7; -.
DR IntAct; Q9VZM7; 10.
DR STRING; 7227.FBpp0073007; -.
DR PaxDb; Q9VZM7; -.
DR PRIDE; Q9VZM7; -.
DR DNASU; 38444; -.
DR EnsemblMetazoa; FBtr0073148; FBpp0073007; FBgn0035462.
DR GeneID; 38444; -.
DR KEGG; dme:Dmel_CG1120; -.
DR UCSC; CG1120-RA; d. melanogaster.
DR CTD; 55174; -.
DR FlyBase; FBgn0035462; IntS10.
DR VEuPathDB; VectorBase:FBgn0035462; -.
DR eggNOG; ENOG502QQ28; Eukaryota.
DR GeneTree; ENSGT00390000010950; -.
DR HOGENOM; CLU_023740_0_0_1; -.
DR InParanoid; Q9VZM7; -.
DR OMA; NQHTELW; -.
DR OrthoDB; 732482at2759; -.
DR PhylomeDB; Q9VZM7; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 38444; 0 hits in 1 CRISPR screen.
DR ChiTaRS; IntS10; fly.
DR GenomeRNAi; 38444; -.
DR PRO; PR:Q9VZM7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035462; Expressed in egg cell and 21 other tissues.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR026164; Int_cplx_su10.
DR PANTHER; PTHR16055; PTHR16055; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..631
FT /note="Integrator complex subunit 10"
FT /id="PRO_0000437662"
FT REGION 545..577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 545..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 631 AA; 72257 MW; 4C25398E7408F0AF CRC64;
MPSQEENELY MVKEAQRLRK SDPCAAMAWI ITAKTLYPNA FNLQYEAYLL ERDAQNYEEA
AKCFSAIATN FQNQHTELWQ EINSLTNALR NENETTPEHE FYVKMYKHLT PEVQHNIFMH
TINHSADNLE RIYIYILMFN KFPKSAITQA PRLLEMLAEG MKTEPDLYQR ILVEEVLPMI
QNKPPELSPN LACRLYTSSL EFYLRQIMDE SDTADAWKNI FKVLMICGQM MGWEPFLPFS
KHVNQNVYWE KLVDILSGSP AGSSQVLFYA TTLFIYSLHG YIRNCKLRIE DADVTHVLVE
GFMEWSPEGD GSEVPSMEPP KFSLTTAISP ELSKAFLHAA QCWQLLNTDQ FQRDFSQLML
ALPLAPWISR FLFDLAIYFG HRDEANKLMA DMTTQSSLVQ SLQILSLNLM QGSMTLQGFQ
CILKILSELP TTQGQLLENM SLKGHRHMVF LPLTRSALVQ YCVGAIISRL SRKVFEPNVP
DRLLGDILVL QQLNLLNDVL LTQQIFNLIK QRKSFNLRTL STYIINIDLL EELSHIWNSQ
QEDNFELTSS PNSSGTPTAT TVAGGSQSRR IGTRGADKGA RDEFRAITRQ QIARCNENVI
TLLANFINQE HLMLAQHIFG ISQPVETIVI K
