INT10_HUMAN
ID INT10_HUMAN Reviewed; 710 AA.
AC Q9NVR2; Q6IA93; Q7L538; Q7L8C8; Q9H3W8;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Integrator complex subunit 10;
DE Short=Int10;
GN Name=INTS10; Synonyms=C8orf35;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 74-710.
RC TISSUE=Lung, and Neuroblastoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-710.
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 377-710.
RC TISSUE=Lymph node;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR
RP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=16239144; DOI=10.1016/j.cell.2005.08.019;
RA Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N.,
RA Shiekhattar R.;
RT "Integrator, a multiprotein mediator of small nuclear RNA processing,
RT associates with the C-terminal repeat of RNA polymerase II.";
RL Cell 123:265-276(2005).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23904267; DOI=10.1091/mbc.e13-05-0254;
RA Jodoin J.N., Sitaram P., Albrecht T.R., May S.B., Shboul M., Lee E.,
RA Reversade B., Wagner E.J., Lee L.A.;
RT "Nuclear-localized Asunder regulates cytoplasmic dynein localization via
RT its role in the integrator complex.";
RL Mol. Biol. Cell 24:2954-2965(2013).
RN [11]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-464, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25114211; DOI=10.1073/pnas.1413825111;
RA Impens F., Radoshevich L., Cossart P., Ribet D.;
RT "Mapping of SUMO sites and analysis of SUMOylation changes induced by
RT external stimuli.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:12432-12437(2014).
RN [12]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-464, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). May
CC be not involved in the recruitment of cytoplasmic dynein to the nuclear
CC envelope by different components of the INT complex (PubMed:23904267).
CC {ECO:0000269|PubMed:23904267, ECO:0000305|PubMed:16239144}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12. Interacts with POLR2A.
CC {ECO:0000269|PubMed:16239144}.
CC -!- INTERACTION:
CC Q9NVR2; Q96SY0: INTS14; NbExp=12; IntAct=EBI-536703, EBI-4409724;
CC Q9NVR2; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-536703, EBI-16439278;
CC Q9NVR2; Q7Z6G3-2: NECAB2; NbExp=3; IntAct=EBI-536703, EBI-10172876;
CC Q9NVR2; Q8N720: ZNF655; NbExp=3; IntAct=EBI-536703, EBI-625509;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16239144,
CC ECO:0000269|PubMed:23904267}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA91687.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK001431; BAA91687.1; ALT_FRAME; mRNA.
DR EMBL; BC001915; AAH01915.2; -; mRNA.
DR EMBL; BC006209; AAH06209.2; -; mRNA.
DR EMBL; BC051282; AAH51282.2; -; mRNA.
DR EMBL; CR457262; CAG33543.1; -; mRNA.
DR EMBL; AL442078; CAC09444.1; -; mRNA.
DR EMBL; BK005727; DAA05727.1; ALT_FRAME; mRNA.
DR CCDS; CCDS6011.2; -.
DR RefSeq; NP_060612.2; NM_018142.2.
DR AlphaFoldDB; Q9NVR2; -.
DR BioGRID; 120473; 42.
DR ComplexPortal; CPX-6441; Integrator complex.
DR CORUM; Q9NVR2; -.
DR DIP; DIP-34063N; -.
DR IntAct; Q9NVR2; 22.
DR MINT; Q9NVR2; -.
DR STRING; 9606.ENSP00000381064; -.
DR iPTMnet; Q9NVR2; -.
DR PhosphoSitePlus; Q9NVR2; -.
DR BioMuta; INTS10; -.
DR DMDM; 97052274; -.
DR EPD; Q9NVR2; -.
DR jPOST; Q9NVR2; -.
DR MassIVE; Q9NVR2; -.
DR MaxQB; Q9NVR2; -.
DR PaxDb; Q9NVR2; -.
DR PeptideAtlas; Q9NVR2; -.
DR PRIDE; Q9NVR2; -.
DR ProteomicsDB; 82848; -.
DR Antibodypedia; 41921; 223 antibodies from 26 providers.
DR DNASU; 55174; -.
DR Ensembl; ENST00000397977.8; ENSP00000381064.3; ENSG00000104613.12.
DR GeneID; 55174; -.
DR KEGG; hsa:55174; -.
DR MANE-Select; ENST00000397977.8; ENSP00000381064.3; NM_018142.4; NP_060612.2.
DR UCSC; uc003wzj.4; human.
DR CTD; 55174; -.
DR DisGeNET; 55174; -.
DR GeneCards; INTS10; -.
DR HGNC; HGNC:25548; INTS10.
DR HPA; ENSG00000104613; Low tissue specificity.
DR MIM; 611353; gene.
DR neXtProt; NX_Q9NVR2; -.
DR OpenTargets; ENSG00000104613; -.
DR PharmGKB; PA142672354; -.
DR VEuPathDB; HostDB:ENSG00000104613; -.
DR eggNOG; ENOG502QQ28; Eukaryota.
DR GeneTree; ENSGT00390000010950; -.
DR HOGENOM; CLU_023740_0_0_1; -.
DR InParanoid; Q9NVR2; -.
DR OMA; NQHTELW; -.
DR OrthoDB; 732482at2759; -.
DR PhylomeDB; Q9NVR2; -.
DR TreeFam; TF323935; -.
DR PathwayCommons; Q9NVR2; -.
DR Reactome; R-HSA-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; Q9NVR2; -.
DR SIGNOR; Q9NVR2; -.
DR BioGRID-ORCS; 55174; 379 hits in 1090 CRISPR screens.
DR ChiTaRS; INTS10; human.
DR GenomeRNAi; 55174; -.
DR Pharos; Q9NVR2; Tbio.
DR PRO; PR:Q9NVR2; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q9NVR2; protein.
DR Bgee; ENSG00000104613; Expressed in body of pancreas and 182 other tissues.
DR ExpressionAtlas; Q9NVR2; baseline and differential.
DR Genevisible; Q9NVR2; HS.
DR GO; GO:0032039; C:integrator complex; IDA:HGNC.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0016180; P:snRNA processing; IDA:HGNC.
DR InterPro; IPR026164; Int_cplx_su10.
DR PANTHER; PTHR16055; PTHR16055; 1.
DR PRINTS; PR02106; INTSUBUNIT10.
PE 1: Evidence at protein level;
KW Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..710
FT /note="Integrator complex subunit 10"
FT /id="PRO_0000235687"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 381
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K2A7"
FT MOD_RES 382
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K2A7"
FT CROSSLNK 464
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25114211,
FT ECO:0007744|PubMed:28112733"
FT CONFLICT 412
FT /note="S -> N (in Ref. 3; CAG33543)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 710 AA; 82236 MW; 3E9945064EC4BEFB CRC64;
MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG
RLLYDMFVNF PDQPVVWREI SIITSALRND SQDKQTQFLR SLFETLPGRV QCEMLLKVTE
QCFNTLERSE MLLLLLRRFP ETVVQHGVGL GEALLEAETI EEQESPVNCF RKLFVCDVLP
LIINNHDVRL PANLLYKYLN KAAEFYINYV TRSTQIENQH QGAQDTSDLM SPSKRSSQKY
IIEGLTEKSS QIVDPWERLF KILNVVGMRC EWQMDKGRRS YGDILHRMKD LCRYMNNFDS
EAHAKYKNQV VYSTMLVFFK NAFQYVNSIQ PSLFQGPNAP SQVPLVLLED VSNVYGDVEI
DRNKHIHKKR KLAEGREKTM SSDDEDCSAK GRNRHIVVNK AELANSTEVL ESFKLARESW
ELLYSLEFLD KEFTRICLAW KTDTWLWLRI FLTDMIIYQG QYKKAIASLH HLAALQGSIS
QPQITGQGTL EHQRALIQLA TCHFALGEYR MTCEKVLDLM CYMVLPIQDG GKSQEEPSKV
KPKFRKGSDL KLLPCTSKAI MPYCLHLMLA CFKLRAFTDN RDDMALGHVI VLLQQEWPRG
ENLFLKAVNK ICQQGNFQYE NFFNYVTNID MLEEFAYLRT QEGGKIHLEL LPNQGMLIKH
HTVTRGITKG VKEDFRLAME RQVSRCGENL MVVLHRFCIN EKILLLQTLT
