INT10_MOUSE
ID INT10_MOUSE Reviewed; 710 AA.
AC Q8K2A7; Q8BYC2; Q8BZI9; Q9D5U1;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 3.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Integrator complex subunit 10;
DE Short=Int10;
GN Name=Ints10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 294-307, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-382, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May be not
CC involved in the recruitment of cytoplasmic dynein to the nuclear
CC envelope by different components of the INT complex.
CC {ECO:0000250|UniProtKB:Q9NVR2}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12. Interacts with POLR2A.
CC {ECO:0000250|UniProtKB:Q9NVR2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NVR2}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC30632.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK034498; BAC28731.1; -; mRNA.
DR EMBL; AK014939; BAB29629.2; -; mRNA.
DR EMBL; AK040574; BAC30632.1; ALT_INIT; mRNA.
DR EMBL; BC031923; AAH31923.2; -; mRNA.
DR CCDS; CCDS22342.1; -.
DR RefSeq; NP_001280720.1; NM_001293791.1.
DR RefSeq; NP_001280721.1; NM_001293792.1.
DR RefSeq; NP_081866.1; NM_027590.4.
DR AlphaFoldDB; Q8K2A7; -.
DR BioGRID; 214319; 13.
DR STRING; 10090.ENSMUSP00000034328; -.
DR iPTMnet; Q8K2A7; -.
DR PhosphoSitePlus; Q8K2A7; -.
DR EPD; Q8K2A7; -.
DR MaxQB; Q8K2A7; -.
DR PaxDb; Q8K2A7; -.
DR PeptideAtlas; Q8K2A7; -.
DR PRIDE; Q8K2A7; -.
DR ProteomicsDB; 267144; -.
DR Antibodypedia; 41921; 223 antibodies from 26 providers.
DR DNASU; 70885; -.
DR Ensembl; ENSMUST00000034328; ENSMUSP00000034328; ENSMUSG00000031864.
DR GeneID; 70885; -.
DR KEGG; mmu:70885; -.
DR UCSC; uc009lwl.2; mouse.
DR CTD; 55174; -.
DR MGI; MGI:1918135; Ints10.
DR VEuPathDB; HostDB:ENSMUSG00000031864; -.
DR eggNOG; ENOG502QQ28; Eukaryota.
DR GeneTree; ENSGT00390000010950; -.
DR HOGENOM; CLU_023740_0_0_1; -.
DR InParanoid; Q8K2A7; -.
DR OrthoDB; 732482at2759; -.
DR PhylomeDB; Q8K2A7; -.
DR TreeFam; TF323935; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 70885; 19 hits in 74 CRISPR screens.
DR ChiTaRS; Ints10; mouse.
DR PRO; PR:Q8K2A7; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8K2A7; protein.
DR Bgee; ENSMUSG00000031864; Expressed in spermatid and 245 other tissues.
DR ExpressionAtlas; Q8K2A7; baseline and differential.
DR Genevisible; Q8K2A7; MM.
DR GO; GO:0032039; C:integrator complex; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0016180; P:snRNA processing; ISO:MGI.
DR InterPro; IPR026164; Int_cplx_su10.
DR PANTHER; PTHR16055; PTHR16055; 1.
DR PRINTS; PR02106; INTSUBUNIT10.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Isopeptide bond; Nucleus; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..710
FT /note="Integrator complex subunit 10"
FT /id="PRO_0000235689"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVR2"
FT MOD_RES 381
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 382
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 464
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NVR2"
FT CONFLICT 304
FT /note="N -> T (in Ref. 2; AAH31923)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="I -> V (in Ref. 2; AAH31923)"
FT /evidence="ECO:0000305"
FT CONFLICT 659
FT /note="K -> KPSSPPMGLLQQEFLPVLQPSIQTADR (in Ref. 1;
FT BAC28731)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 710 AA; 82020 MW; EC54D7582EABA77E CRC64;
MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG
RLLYDMFVNF PDQPVVWREI SIITSALRND SQDKQTQFLR SLFETLPGRV QCEMLLKVTE
QCFNTLERSE MLLLLLRRFP ETVVQHGVGL GEALLEAETI EEQDSPVNCF RKLFVCDVLP
LIINNHDVRL PANLLYKYLN KAAEFYINYV TRSTQSENQH QGAQDTSDLM SPSKRSSQKY
IIEGLTEKSS HIVDPWERLF KILNVVGMRC EWQMDKGRRS CSDLLHRMKE LCRYMNSFDS
EAHNNYKNQV LYSTMLVFFK SAFQYVSSIQ PSLFQGPNAP SQVPLILLED VANVYGDVEI
DRSKHIHKKR KLAEGREKTM SSDDEECSAK GRNRHIVVSK ADLSNSIEVL ESFKLARESW
ELLYSLEFLD KEFTRICLAW KTDTWLWLRI FLTDMIIYQG QYKKAIASLH HLAALQGSLS
QPQITGQGTL EHQRALIQLA TCHFALGEYR MTCEKVLDLM CYMVLPIQDG GKPQEEPSKV
KPKCRKGLDL KLLPCTSKAI MPYCLHLMLA CFKLRAFTDS RDDMALGHVI VLLQQEWPRG
ENLFLKAISK ICQQGNFQYE NFFSYVTNID MLEEFAYLRT QEGGKIHLEL LPNQGMLIKH
HTVTRGITKG VKEDFRLAME RQVSRCGENL MAVLHRFCIN EKILLLQTLT
