INT11_DROME
ID INT11_DROME Reviewed; 597 AA.
AC Q9VAH9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Integrator complex subunit 11 {ECO:0000303|PubMed:21078872};
DE EC=3.1.27.- {ECO:0000305};
GN Name=IntS11 {ECO:0000312|FlyBase:FBgn0039691};
GN ORFNames=CG1972 {ECO:0000312|FlyBase:FBgn0039691};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL28645.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL28645.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL28645.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, SUBUNIT, AND INTERACTION WITH INTS12.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Catalytic component of the Integrator complex, a complex
CC involved in the transcription of small nuclear RNAs (snRNA) and their
CC 3'-box-dependent processing (By similarity). Mediates the snRNAs 3'
CC cleavage (By similarity). Involved in the 3'-end processing of the U7
CC snRNA, and also the spliceosomal snRNAs U1, U2, U4 and U5
CC (PubMed:21078872, PubMed:23097424). May mediate recruitment of
CC cytoplasmic dynein to the nuclear envelope, probably as component of
CC the INT complex (By similarity). {ECO:0000250|UniProtKB:Q5TA45,
CC ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14. Within
CC the complex, interacts with IntS12. {ECO:0000269|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5TA45}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q5TA45}.
CC -!- DOMAIN: The HXHXDH motif is essential for the endoribonuclease activity
CC of the CPSF complex. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-
CC metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF56931.1; -; Genomic_DNA.
DR EMBL; AY061097; AAL28645.1; -; mRNA.
DR RefSeq; NP_651721.1; NM_143464.4.
DR AlphaFoldDB; Q9VAH9; -.
DR SMR; Q9VAH9; -.
DR IntAct; Q9VAH9; 1.
DR STRING; 7227.FBpp0084842; -.
DR PaxDb; Q9VAH9; -.
DR PRIDE; Q9VAH9; -.
DR DNASU; 43506; -.
DR EnsemblMetazoa; FBtr0085476; FBpp0084842; FBgn0039691.
DR GeneID; 43506; -.
DR KEGG; dme:Dmel_CG1972; -.
DR UCSC; CG1972-RA; d. melanogaster.
DR CTD; 54973; -.
DR FlyBase; FBgn0039691; IntS11.
DR VEuPathDB; VectorBase:FBgn0039691; -.
DR eggNOG; KOG1136; Eukaryota.
DR GeneTree; ENSGT00940000157644; -.
DR HOGENOM; CLU_009673_3_2_1; -.
DR InParanoid; Q9VAH9; -.
DR OMA; YLDGMIW; -.
DR OrthoDB; 218195at2759; -.
DR PhylomeDB; Q9VAH9; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 43506; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 43506; -.
DR PRO; PR:Q9VAH9; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039691; Expressed in egg cell and 26 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0004521; F:endoribonuclease activity; ISS:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR CDD; cd16291; INTS11-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR022712; Beta_Casp.
DR InterPro; IPR041897; INTS11-like_MBL-fold.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR InterPro; IPR011108; RMMBL.
DR Pfam; PF10996; Beta-Casp; 1.
DR Pfam; PF16661; Lactamase_B_6; 1.
DR Pfam; PF07521; RMMBL; 1.
DR SMART; SM01027; Beta-Casp; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Nucleus; Reference proteome.
FT CHAIN 1..597
FT /note="Integrator complex subunit 11"
FT /id="PRO_0000437670"
FT MOTIF 68..73
FT /note="HXHXDH motif"
FT /evidence="ECO:0000305"
SQ SEQUENCE 597 AA; 67592 MW; DFB0241D8C5AAA93 CRC64;
MPDIKITPLG AGQDVGRSCL LLSMGGKNIM LDCGMHMGYN DERRFPDFSY IVPEGPITSH
IDCVIISHFH LDHCGALPYM SEIVGYTGPI YMTHPTKAIA PILLEDMRKV AVERKGESNF
FTTQMIKDCM KKVIPVTLHQ SMMVDTDLEI KAYYAGHVLG AAMFWIKVGS QSVVYTGDYN
MTPDRHLGAA WIDKCRPDLL ISESTYATTI RDSKRCRERD FLKKVHECVA KGGKVLIPVF
ALGRAQELCI LLETYWERMN LKYPIYFALG LTEKANTYYK MFITWTNQKI RKTFVHRNMF
DFKHIKPFDK AYIDNPGAMV VFATPGMLHA GLSLQIFKKW APNENNMVIM PGYCVQGTVG
NKILGGAKKV EFENRQVVEV KMAVEYMSFS AHADAKGIMQ LIQNCEPKNV MLVHGEAGKM
KFLRSKIKDE FNLETYMPAN GETCVISTPV KIPVDASVSL LKAEARSYNA QPPDPKRRRL
IHGVLVMKDN RIMLQNLTDA LKEIGINRHV MRFTSKVKMD DSGPVIRTSE RLKTLLEEKL
AGWTVTMQEN GSIAIESVEV KVEEDEKDPK QKNILISWTN QDEDIGAYIL NVLQNMC
