INT11_MOUSE
ID INT11_MOUSE Reviewed; 600 AA.
AC Q9CWS4; Q3TJ34; Q3U7B0; Q91X99; Q922F0;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Integrator complex subunit 11;
DE Short=Int11;
DE EC=3.1.27.-;
DE AltName: Full=Cleavage and polyadenylation-specific factor 3-like protein;
DE Short=CPSF3-like protein;
GN Name=Ints11; Synonyms=Cpsf3l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Bone marrow, Placenta, Seminal vesicle, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Catalytic component of the Integrator complex, a complex
CC involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and
CC in their 3'-box-dependent processing. The Integrator complex is
CC associated with the C-terminal domain (CTD) of RNA polymerase II
CC largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs
CC genes. Mediates the snRNAs 3' cleavage. Mediates recruitment of
CC cytoplasmic dynein to the nuclear envelope, probably as component of
CC the INT complex. {ECO:0000250|UniProtKB:Q5TA45}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.
CC {ECO:0000250|UniProtKB:Q5TA45}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5TA45}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q5TA45}.
CC -!- DOMAIN: The HXHXDH motif is essential for the endoribonuclease activity
CC of the CPSF complex. {ECO:0000250|UniProtKB:Q9VAH9}.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-
CC metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH08240.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK010425; BAB26928.1; -; mRNA.
DR EMBL; AK090206; BAC41135.1; -; mRNA.
DR EMBL; AK150436; BAE29558.1; -; mRNA.
DR EMBL; AK152740; BAE31460.1; -; mRNA.
DR EMBL; AK167607; BAE39661.1; -; mRNA.
DR EMBL; AK172533; BAE43054.1; -; mRNA.
DR EMBL; BC008240; AAH08240.1; ALT_INIT; mRNA.
DR EMBL; BC011155; AAH11155.1; -; mRNA.
DR CCDS; CCDS19048.1; -.
DR RefSeq; NP_082296.1; NM_028020.3.
DR RefSeq; XP_006539258.1; XM_006539195.2.
DR AlphaFoldDB; Q9CWS4; -.
DR SMR; Q9CWS4; -.
DR STRING; 10090.ENSMUSP00000030901; -.
DR iPTMnet; Q9CWS4; -.
DR PhosphoSitePlus; Q9CWS4; -.
DR EPD; Q9CWS4; -.
DR MaxQB; Q9CWS4; -.
DR PaxDb; Q9CWS4; -.
DR PRIDE; Q9CWS4; -.
DR ProteomicsDB; 268973; -.
DR Antibodypedia; 26257; 201 antibodies from 26 providers.
DR DNASU; 71957; -.
DR Ensembl; ENSMUST00000030901; ENSMUSP00000030901; ENSMUSG00000029034.
DR GeneID; 71957; -.
DR KEGG; mmu:71957; -.
DR UCSC; uc008wfh.1; mouse.
DR CTD; 54973; -.
DR MGI; MGI:1919207; Ints11.
DR VEuPathDB; HostDB:ENSMUSG00000029034; -.
DR eggNOG; KOG1136; Eukaryota.
DR GeneTree; ENSGT00940000157644; -.
DR HOGENOM; CLU_009673_3_2_1; -.
DR InParanoid; Q9CWS4; -.
DR OMA; YLDGMIW; -.
DR OrthoDB; 218195at2759; -.
DR PhylomeDB; Q9CWS4; -.
DR TreeFam; TF105878; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 71957; 27 hits in 73 CRISPR screens.
DR ChiTaRS; Ints11; mouse.
DR PRO; PR:Q9CWS4; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9CWS4; protein.
DR Bgee; ENSMUSG00000029034; Expressed in ectoplacental cone and 247 other tissues.
DR ExpressionAtlas; Q9CWS4; baseline and differential.
DR Genevisible; Q9CWS4; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0004521; F:endoribonuclease activity; ISO:MGI.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC.
DR CDD; cd16291; INTS11-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR022712; Beta_Casp.
DR InterPro; IPR041897; INTS11-like_MBL-fold.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR InterPro; IPR011108; RMMBL.
DR Pfam; PF10996; Beta-Casp; 1.
DR Pfam; PF16661; Lactamase_B_6; 1.
DR Pfam; PF07521; RMMBL; 1.
DR SMART; SM01027; Beta-Casp; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Nucleus; Reference proteome.
FT CHAIN 1..600
FT /note="Integrator complex subunit 11"
FT /id="PRO_0000259564"
FT MOTIF 68..73
FT /note="HXHXDH motif"
FT CONFLICT 136
FT /note="V -> D (in Ref. 2; AAH11155)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="R -> G (in Ref. 1; BAE31460)"
FT /evidence="ECO:0000305"
FT CONFLICT 542
FT /note="C -> R (in Ref. 1; BAE39661)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 600 AA; 67844 MW; 1976D09E065BE2FA CRC64;
MPEIRVTPLG AGQDVGRSCI LVSISGKNVM LDCGMHMGYN DDRRFPDFSY ITQSGRLTDF
LDCVIISHFH LDHCGALPYF SEMVGYDGPI YMTHPTQAIC PILLEDYRKI AVDKKGEANF
FTSQMIKDCM KKVVAVHLHQ TVQVDDELEI KAYYAGHVLG AAMFQIKVGS ESVVYTGDYN
MTPDRHLGAA WIDKCRPNLL ITESTYATTI RDSKRCRERD FLKKVHETVE RGGKVLIPVF
ALGRAQELCI LLETFWERMN LKVPIYFSTG LTEKANHYYK LFITWTNQKI RKTFVQRNMF
EFKHIKAFDR TFADNPGPMV VFATPGMLHA GQSLQIFRKW AGNEKNMVIM PGYCVQGTVG
HKILSGQRKL EMEGRQMLEV KMQVEYMSFS AHADAKGIMQ LVGQAEPESV LLVHGEAKKM
EFLRQKIEQE FRVSCYMPAN GETVTLPTSP SIPVGISLGL LKREMVQGLL PEAKKPRLLH
GTLIMKDSNF RLVSSEQALK ELGLAEHQLR FTCRVHLQDT RKEQETALRV YSHLKSTLKD
HCVQHLPDGS VTVESILIQA AAHSEDPGTK VLLVSWTYQD EELGSFLTTL LKNGLPQAPS
