INT12_DROME
ID INT12_DROME Reviewed; 328 AA.
AC Q9VBB3;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Integrator complex subunit 12 {ECO:0000303|PubMed:21078872};
GN Name=IntS12 {ECO:0000312|FlyBase:FBgn0039459};
GN ORFNames=CG5491 {ECO:0000312|FlyBase:FBgn0039459};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL48045.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48045.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL48045.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
RN [6] {ECO:0000305}
RP FUNCTION, INTERACTION WITH INTS1 AND INTS9, AND DOMAIN.
RX PubMed=23288851; DOI=10.1074/jbc.m112.425892;
RA Chen J., Waltenspiel B., Warren W.D., Wagner E.J.;
RT "Functional analysis of the integrator subunit 12 identifies a microdomain
RT that mediates activation of the Drosophila integrator complex.";
RL J. Biol. Chem. 288:4867-4877(2013).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424,
CC PubMed:23288851). Involved in the 3'-end processing of the U7 snRNA,
CC and also the spliceosomal snRNAs U1, U2, U4 and U5 (PubMed:21078872,
CC PubMed:23097424, PubMed:23288851). Required for the normal expression
CC of the Integrator complex component IntS1 (PubMed:23288851). May
CC mediate recruitment of cytoplasmic dynein to the nuclear envelope,
CC probably as component of the INT complex (By similarity).
CC {ECO:0000250|UniProtKB:Q96CB8, ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424, ECO:0000269|PubMed:23288851}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14
CC (PubMed:23097424). Within the complex, interacts with IntS1 and IntS9
CC (PubMed:23288851). Interaction with IntS1 is likely to be important for
CC promoting 3'-end processing of snRNAs (PubMed:23288851).
CC {ECO:0000269|PubMed:23288851, ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23288851}.
CC -!- DOMAIN: The PHD-type zinc finger is not required for 3'-end snRNA
CC processing. {ECO:0000269|PubMed:23288851}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 12 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF56629.1; -; Genomic_DNA.
DR EMBL; AY070574; AAL48045.1; -; mRNA.
DR RefSeq; NP_651507.1; NM_143250.3.
DR AlphaFoldDB; Q9VBB3; -.
DR SMR; Q9VBB3; -.
DR IntAct; Q9VBB3; 1.
DR STRING; 7227.FBpp0084433; -.
DR PaxDb; Q9VBB3; -.
DR PRIDE; Q9VBB3; -.
DR DNASU; 43227; -.
DR EnsemblMetazoa; FBtr0085061; FBpp0084433; FBgn0039459.
DR GeneID; 43227; -.
DR KEGG; dme:Dmel_CG5491; -.
DR UCSC; CG5491-RA; d. melanogaster.
DR CTD; 57117; -.
DR FlyBase; FBgn0039459; IntS12.
DR VEuPathDB; VectorBase:FBgn0039459; -.
DR eggNOG; KOG4323; Eukaryota.
DR GeneTree; ENSGT00390000005218; -.
DR HOGENOM; CLU_837488_0_0_1; -.
DR InParanoid; Q9VBB3; -.
DR OMA; MTPRMLE; -.
DR OrthoDB; 1631937at2759; -.
DR PhylomeDB; Q9VBB3; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 43227; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43227; -.
DR PRO; PR:Q9VBB3; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039459; Expressed in cleaving embryo and 23 other tissues.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR CDD; cd15501; PHD_Int12; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039054; Int12_PHD.
DR InterPro; IPR019786; Zinc_finger_PHD-type_CS.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR001965; Znf_PHD.
DR InterPro; IPR019787; Znf_PHD-finger.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00628; PHD; 1.
DR SMART; SM00249; PHD; 1.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS01359; ZF_PHD_1; 1.
DR PROSITE; PS50016; ZF_PHD_2; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..328
FT /note="Integrator complex subunit 12"
FT /id="PRO_0000437663"
FT ZN_FING 128..185
FT /note="PHD-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00146"
FT REGION 1..45
FT /note="Sufficient for binding to IntS1 and IntS9 and for
FT 3'-end snRNA processing"
FT /evidence="ECO:0000269|PubMed:23288851"
FT REGION 215..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..259
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 328 AA; 34334 MW; 0F18EA4EC936BD0C CRC64;
MAANIAAAAA AAQEVDPVLK KAIKLLHSSN PTSAAELRLL LDEALKARFG PEKSLTNNMT
PRMLEDEANF SGRAATPPQQ PINADEIINL TNSPDKEPSD SVDTIADSDD GLSAVGIVNT
GDTGDFGDLN CCVCGEMVFT ATNRLIECSK CGAMYHQECH KPPITKEEAA DDQEQNWQCD
TCCNKPTSSG RTTSSAAAVT PTVFIADEPM PLTSKAKSSV ASSRSSNSSN SSSPFYRPEP
SSSTNASSSS SSKHGHKSSS SSSSKSHKEE RSSKSTAASS LSAIGGMEKH NSSGTSSRRS
GSSTKSSSKS SSSKHHESGS SSKRRSKQ
