INT13_MOUSE
ID INT13_MOUSE Reviewed; 732 AA.
AC Q8QZV7; E9QLV7; Q99JT8;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Integrator complex subunit 13 {ECO:0000312|MGI:MGI:1918427};
DE AltName: Full=Cell cycle regulator Mat89Bb homolog;
DE AltName: Full=Protein asunder homolog;
DE AltName: Full=Spermatogenesis-associated protein 30;
GN Name=IntS13 {ECO:0000312|MGI:MGI:1918427}; Synonyms=Asun, Spata30;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP INTERACTION WITH PAFAH1B1, AND SUBCELLULAR LOCATION.
RX PubMed=23097494; DOI=10.1091/mbc.e12-07-0558;
RA Jodoin J.N., Shboul M., Sitaram P., Zein-Sabatto H., Reversade B., Lee E.,
RA Lee L.A.;
RT "Human Asunder promotes dynein recruitment and centrosomal tethering to the
RT nucleus at mitotic entry.";
RL Mol. Biol. Cell 23:4713-4724(2012).
RN [5]
RP SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF 577-ARG--ASP-582.
RX PubMed=23904267; DOI=10.1091/mbc.e13-05-0254;
RA Jodoin J.N., Sitaram P., Albrecht T.R., May S.B., Shboul M., Lee E.,
RA Reversade B., Wagner E.J., Lee L.A.;
RT "Nuclear-localized Asunder regulates cytoplasmic dynein localization via
RT its role in the integrator complex.";
RL Mol. Biol. Cell 24:2954-2965(2013).
CC -!- FUNCTION: Crucial regulator of the mitotic cell cycle and development.
CC At prophase, required for dynein anchoring to the nuclear envelope
CC important for proper centrosome-nucleus coupling. At G2/M phase, may be
CC required for proper spindle formation and execution of cytokinesis.
CC Probable component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. {ECO:0000250|UniProtKB:Q9NVM9}.
CC -!- SUBUNIT: Interacts with PAFAH1B1; this interaction may be required for
CC proper recruitment of dynein complexes to the nuclear envelope at
CC prophase. {ECO:0000269|PubMed:23097494}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23904267}. Cytoplasm
CC {ECO:0000269|PubMed:23097494, ECO:0000269|PubMed:23904267}.
CC Note=Nuclear location is required for recruitment of dynein motors to
CC nuclear envelope at G2/M. {ECO:0000250|UniProtKB:Q9NVM9}.
CC -!- SIMILARITY: Belongs to the asunder family. {ECO:0000305}.
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DR EMBL; AC124128; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC005696; AAH05696.1; -; mRNA.
DR EMBL; BC026851; AAH26851.1; -; mRNA.
DR EMBL; BC027229; AAH27229.1; -; mRNA.
DR CCDS; CCDS39710.1; -.
DR RefSeq; NP_620096.2; NM_138757.2.
DR AlphaFoldDB; Q8QZV7; -.
DR SMR; Q8QZV7; -.
DR BioGRID; 214533; 3.
DR IntAct; Q8QZV7; 1.
DR MINT; Q8QZV7; -.
DR STRING; 10090.ENSMUSP00000032427; -.
DR iPTMnet; Q8QZV7; -.
DR PhosphoSitePlus; Q8QZV7; -.
DR EPD; Q8QZV7; -.
DR jPOST; Q8QZV7; -.
DR MaxQB; Q8QZV7; -.
DR PaxDb; Q8QZV7; -.
DR PeptideAtlas; Q8QZV7; -.
DR PRIDE; Q8QZV7; -.
DR ProteomicsDB; 268975; -.
DR Antibodypedia; 42367; 70 antibodies from 20 providers.
DR DNASU; 71177; -.
DR Ensembl; ENSMUST00000032427; ENSMUSP00000032427; ENSMUSG00000040250.
DR GeneID; 71177; -.
DR KEGG; mmu:71177; -.
DR UCSC; uc009esb.1; mouse.
DR CTD; 55726; -.
DR MGI; MGI:1918427; Ints13.
DR VEuPathDB; HostDB:ENSMUSG00000040250; -.
DR eggNOG; KOG3711; Eukaryota.
DR GeneTree; ENSGT00390000002793; -.
DR InParanoid; Q8QZV7; -.
DR OMA; CMDEAPS; -.
DR OrthoDB; 676663at2759; -.
DR PhylomeDB; Q8QZV7; -.
DR TreeFam; TF105815; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 71177; 23 hits in 77 CRISPR screens.
DR ChiTaRS; Ints13; mouse.
DR PRO; PR:Q8QZV7; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8QZV7; protein.
DR Bgee; ENSMUSG00000040250; Expressed in otic placode and 254 other tissues.
DR ExpressionAtlas; Q8QZV7; baseline and differential.
DR Genevisible; Q8QZV7; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0032039; C:integrator complex; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051642; P:centrosome localization; ISO:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0007052; P:mitotic spindle organization; ISO:MGI.
DR GO; GO:0090435; P:protein localization to nuclear envelope; ISO:MGI.
DR GO; GO:0080154; P:regulation of fertilization; ISS:UniProtKB.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; ISS:UniProtKB.
DR InterPro; IPR019355; Cell_cycle_regulator_Mat89Bb.
DR PANTHER; PTHR12955; PTHR12955; 1.
DR Pfam; PF10221; DUF2151; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Isopeptide bond; Mitosis; Nucleus;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..732
FT /note="Integrator complex subunit 13"
FT /id="PRO_0000089846"
FT REGION 564..650
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 572..582
FT /note="Nuclear localization signal (NLS)"
FT /evidence="ECO:0000269|PubMed:23904267"
FT COMPBIAS 564..646
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 623
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVM9"
FT MOD_RES 626
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVM9"
FT CROSSLNK 611
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NVM9"
FT MUTAGEN 577..582
FT /note="REDRED->AAAAAA: Loss of nuclear location. Location
FT is mainly cytoplasmic or diffuse."
FT /evidence="ECO:0000269|PubMed:23904267"
FT CONFLICT 303
FT /note="S -> G (in Ref. 2; AAH26851/AAH27229)"
FT /evidence="ECO:0000305"
FT CONFLICT 605
FT /note="G -> R (in Ref. 2; AAH05696)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 732 AA; 82780 MW; 36E92DC5C00D77F3 CRC64;
MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM
EYCRIMYDIF PFKKLVNFIV SDSGAHVLNS WTQEDQNLQE LMAALATVGP PNPRADPECC
SILHGLVAAV ETLCKITEYQ HEARTLLMEN AERVGNRGRI ICITNAKSDS HVRMLEDCVQ
ETIHEHNKLA ANSDHLMQIQ KCELVLIHTY PVGEDSLVSD RPKKELSPVL TSEVHSVRAG
RHLATKLNVL VQQHFDLAST TITNIPMKEE QHANTSANYD VELLHHKDAH VDFLRSGDSH
SGSSSREGPF KETVTLKWCT PRTNSIELHY CTGAYRISPV DVNSRPSSCL TNFLLNGRSV
LLEQPRKSGS KVISHMLSSH GGEIFLHVLS SSRSILEDPP SISEGCGGRV TDYRITDFGE
FMRENRLTPF LDPRYKIDAS LEIPLERAKD QLEKHTRYWP MIISQTTIFN MQAVVPLAGV
IVKESLTEED VLNCQKTIYN LVDMERKNDP LPVSTVGTRG KGPKRDEQYR IMWNELETLV
RAHISNSEKH QRVLECLMAC RSKPPEEEER KKRGRKREDR EDKSEQAGKE HGTEKARPDA
DRLKGILERG KEELAEAEVI KDSPDSPEPP NKKPLVETDE TPHMEKSKGP VSLLSLWSNR
INTANSRKHQ EFAGRLNSVN NRAELYQHLK EENGEEVGLT GGPRTAIPFL KCPRSPVVNR
METTENGKAS RQ
