INT14_DROME
ID INT14_DROME Reviewed; 587 AA.
AC Q9VPY0;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Integrator complex subunit 14 {ECO:0000303|PubMed:23097424};
GN Name=IntS14 {ECO:0000312|FlyBase:FBgn0031314};
GN ORFNames=CG4785 {ECO:0000312|FlyBase:FBgn0031314};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM49906.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM49906.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM49906.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, AND IDENTIFICATION IN THE
RP INTEGRATOR COMPLEX.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing. Involved in the 3'-end processing of the U7
CC snRNA, and also the spliceosomal snRNAs U1 and U5.
CC {ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23097424}.
CC -!- SIMILARITY: Belongs to the INTS14 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF51404.1; -; Genomic_DNA.
DR EMBL; AY118537; AAM49906.1; -; mRNA.
DR RefSeq; NP_608579.1; NM_134735.2.
DR AlphaFoldDB; Q9VPY0; -.
DR SMR; Q9VPY0; -.
DR IntAct; Q9VPY0; 2.
DR STRING; 7227.FBpp0077627; -.
DR PaxDb; Q9VPY0; -.
DR PRIDE; Q9VPY0; -.
DR DNASU; 33300; -.
DR EnsemblMetazoa; FBtr0077962; FBpp0077627; FBgn0031314.
DR GeneID; 33300; -.
DR KEGG; dme:Dmel_CG4785; -.
DR UCSC; CG4785-RA; d. melanogaster.
DR CTD; 81556; -.
DR FlyBase; FBgn0031314; IntS14.
DR VEuPathDB; VectorBase:FBgn0031314; -.
DR eggNOG; ENOG502QQ37; Eukaryota.
DR GeneTree; ENSGT00390000009486; -.
DR HOGENOM; CLU_041485_0_0_1; -.
DR InParanoid; Q9VPY0; -.
DR OMA; WIRQASL; -.
DR OrthoDB; 1453503at2759; -.
DR PhylomeDB; Q9VPY0; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; Q9VPY0; -.
DR BioGRID-ORCS; 33300; 0 hits in 1 CRISPR screen.
DR ChiTaRS; IntS14; fly.
DR GenomeRNAi; 33300; -.
DR PRO; PR:Q9VPY0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031314; Expressed in secondary oocyte and 20 other tissues.
DR GO; GO:0032039; C:integrator complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR039841; INTS14.
DR InterPro; IPR045814; IntS14_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR13532; PTHR13532; 1.
DR Pfam; PF19435; IntS14; 1.
DR Pfam; PF13519; VWA_2; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..587
FT /note="Integrator complex subunit 14"
FT /id="PRO_0000437671"
FT DOMAIN 3..113
FT /note="VWFA"
FT /evidence="ECO:0000255"
FT REGION 190..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 587 AA; 65049 MW; 02E61FF44BA8B53A CRC64;
MPTLIALDAS LSMLRPVPGR NEHTYQSLAT KGIQHLLDNL TAAGKLEHVA LLSYSTTAEL
KVDFTRDYDQ VRQAVKKVEP VDKACLMSML KAVVSIMSPW GNQNILQVVV FTDCGLGFGN
TSITGFLEAY AEKESEPEFG FLKTLANYNL NFICLGLHGD YYFTRGLAVY QQLLDKVSLK
GQLFMTKPAK SSDAVEGNPN PNPNPSHKSE LGRTTVFELI ERLCEASYKS SEVTLKCGSY
FRMEASVLLW PPTAPYEQKS HIFGREPTIR HTDQKIEVCG FLSLSDIGSP ATLSRHWVLP
KVEREKSGSS RRSGNLSAAA KPPKLNLDTS NPNYELEKLE ADIKEFYAKD SKDTEESGDD
DVTIVLKPGP QTEQQKENLC VLLHGALKME NMAALVRVGD KWYGFIYAFT DSKKKSNLML
NILPPGTNVI PWLGDLESLG FPEDLAPGET ASFPVRADRR SYSQSSVVWI RQASLQSDVQ
KVLRHAKKMP DKTQHFYKEL NRIRRAALAL GFVELLEALA MLLEKECAHL SLNGASNDCT
LQLQHAATEL RKTSNRDMKS MIVPLQKVGA SDAGSTAATA PAPAYMY
