INT14_MOUSE
ID INT14_MOUSE Reviewed; 515 AA.
AC Q8R3P6; Q3THM0; Q3V323; Q3V3E8; Q8C072;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Integrator complex subunit 14 {ECO:0000312|MGI:MGI:1917132};
DE AltName: Full=von Willebrand factor A domain-containing protein 9 {ECO:0000305};
GN Name=Ints14 {ECO:0000312|MGI:MGI:1917132};
GN Synonyms=Vwa9 {ECO:0000312|MGI:MGI:1917132};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, DBA/2J, and NOD;
RC TISSUE=Medulla oblongata, Thymus, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probable component of the Integrator (INT) complex, a complex
CC involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and
CC in their 3'-box-dependent processing. {ECO:0000250|UniProtKB:Q9VPY0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9VPY0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8R3P6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R3P6-2; Sequence=VSP_027182, VSP_027183;
CC -!- SIMILARITY: Belongs to the INTS14 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC27696.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK032095; BAC27696.1; ALT_FRAME; mRNA.
DR EMBL; AK035654; BAC29140.1; -; mRNA.
DR EMBL; AK041578; BAE20594.1; -; mRNA.
DR EMBL; AK050654; BAE20686.1; -; mRNA.
DR EMBL; AK168222; BAE40176.1; -; mRNA.
DR EMBL; AK169772; BAE41357.1; -; mRNA.
DR EMBL; BC024913; AAH24913.1; -; mRNA.
DR CCDS; CCDS40668.1; -. [Q8R3P6-1]
DR RefSeq; NP_001071099.1; NM_001077631.2. [Q8R3P6-1]
DR RefSeq; NP_780362.2; NM_175153.4. [Q8R3P6-1]
DR AlphaFoldDB; Q8R3P6; -.
DR SMR; Q8R3P6; -.
DR BioGRID; 213732; 3.
DR STRING; 10090.ENSMUSP00000049284; -.
DR iPTMnet; Q8R3P6; -.
DR PhosphoSitePlus; Q8R3P6; -.
DR EPD; Q8R3P6; -.
DR MaxQB; Q8R3P6; -.
DR PaxDb; Q8R3P6; -.
DR PeptideAtlas; Q8R3P6; -.
DR PRIDE; Q8R3P6; -.
DR ProteomicsDB; 269487; -. [Q8R3P6-1]
DR ProteomicsDB; 269488; -. [Q8R3P6-2]
DR Antibodypedia; 52220; 94 antibodies from 17 providers.
DR DNASU; 69882; -.
DR Ensembl; ENSMUST00000037504; ENSMUSP00000049284; ENSMUSG00000034263. [Q8R3P6-1]
DR Ensembl; ENSMUST00000170517; ENSMUSP00000127420; ENSMUSG00000034263. [Q8R3P6-1]
DR GeneID; 69882; -.
DR KEGG; mmu:69882; -.
DR UCSC; uc009qcm.1; mouse. [Q8R3P6-2]
DR UCSC; uc009qcn.2; mouse. [Q8R3P6-1]
DR CTD; 81556; -.
DR MGI; MGI:1917132; Ints14.
DR VEuPathDB; HostDB:ENSMUSG00000034263; -.
DR eggNOG; ENOG502QQ37; Eukaryota.
DR GeneTree; ENSGT00390000009486; -.
DR HOGENOM; CLU_041485_0_0_1; -.
DR InParanoid; Q8R3P6; -.
DR OMA; WIRQASL; -.
DR OrthoDB; 1453503at2759; -.
DR PhylomeDB; Q8R3P6; -.
DR TreeFam; TF323245; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 69882; 24 hits in 74 CRISPR screens.
DR ChiTaRS; Ints14; mouse.
DR PRO; PR:Q8R3P6; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8R3P6; protein.
DR Bgee; ENSMUSG00000034263; Expressed in undifferentiated genital tubercle and 241 other tissues.
DR Genevisible; Q8R3P6; MM.
DR GO; GO:0032039; C:integrator complex; IEA:InterPro.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR039841; INTS14.
DR InterPro; IPR045814; IntS14_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR13532; PTHR13532; 1.
DR Pfam; PF19435; IntS14; 1.
DR Pfam; PF13519; VWA_2; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Nucleus; Reference proteome.
FT CHAIN 1..515
FT /note="Integrator complex subunit 14"
FT /id="PRO_0000296268"
FT DOMAIN 2..204
FT /note="VWFA"
FT MOD_RES 418
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q96SY0"
FT VAR_SEQ 250..257
FT /note="DLEIVGFI -> GNCLKLPS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027182"
FT VAR_SEQ 258..515
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027183"
FT CONFLICT 178
FT /note="L -> I (in Ref. 1; BAC27696)"
FT /evidence="ECO:0000305"
FT CONFLICT 482
FT /note="A -> T (in Ref. 1; BAE40176)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 515 AA; 57237 MW; A7491CA16DBEB9C7 CRC64;
MPTVVVMDVS LSMTRPVSVE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW
ELMVPFTRDY NTLQEALSNM DDYDKTCLES ALVGVCNIVQ QEWGGAIPCQ VVLVTDGCLG
IGRGSLRHSL ATQNQRSESN RFPLPFPFPS KLYVMCMANL EELQSTDSLE CLERLIDLNN
GEGQIFTIDG PLCLKNVQSM FGKLIDLAYT PFHAVLKCGH LTADVQVFPR PEPFVIDEEI
DPIPKVINTD LEIVGFIDIA DISSPPVLSR HLVLPIALNK EGDEVGAGIT DDNEDENSAN
QIAGKIPNFC VLLHGSLKVE GMVALVQLGP EWHGMLYSQA DSKKKSNLMM SLFEPGPEPL
PWLGKMAQLG PISDAKENPY GEDDNKSPFP LQPKNKRSYA QNVTVWIKPS GLQTDVQKIL
RNARKLPEKT QTFYKELNRL RKAALAFGFL DLLKGVADML ERECTLLPDT AHPDAAFQLT
HAAQQLKLAS TEYAIYDHNI TPLHTDFSGS STERM
