INT1_DROME
ID INT1_DROME Reviewed; 2053 AA.
AC Q9W1C5; Q8IGA9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Integrator complex subunit 1 {ECO:0000303|PubMed:21078872};
GN Name=IntS1 {ECO:0000312|FlyBase:FBgn0034964};
GN ORFNames=CG3173 {ECO:0000312|FlyBase:FBgn0034964};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAN71641.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71641.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAN71641.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, SUBUNIT, AND INTERACTION WITH CDK8 AND CYCC.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH INTS12 AND INTS9.
RX PubMed=23288851; DOI=10.1074/jbc.m112.425892;
RA Chen J., Waltenspiel B., Warren W.D., Wagner E.J.;
RT "Functional analysis of the integrator subunit 12 identifies a microdomain
RT that mediates activation of the Drosophila integrator complex.";
RL J. Biol. Chem. 288:4867-4877(2013).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424, PubMed:23288851).
CC Required for the normal expression of the Integrator complex component
CC IntS12 (PubMed:23288851). May mediate recruitment of cytoplasmic dynein
CC to the nuclear envelope, probably as component of the INT complex (By
CC similarity). {ECO:0000250|UniProtKB:Q8N201,
CC ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424,
CC ECO:0000269|PubMed:23288851}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14
CC (PubMed:23097424). Within the complex, interacts with IntS12 and IntS9
CC (PubMed:23288851). Interaction with IntS12 is likely to be important
CC for promoting 3'-end processing of snRNAs (PubMed:23288851). Interacts
CC with Mediator complex members Cdk8 and CycC (PubMed:23097424).
CC {ECO:0000269|PubMed:23097424, ECO:0000269|PubMed:23288851}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:23288851};
CC Single-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 1 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF47145.2; -; Genomic_DNA.
DR EMBL; BT001870; AAN71641.1; -; mRNA.
DR RefSeq; NP_611875.1; NM_138031.3.
DR AlphaFoldDB; Q9W1C5; -.
DR IntAct; Q9W1C5; 12.
DR STRING; 7227.FBpp0072077; -.
DR PaxDb; Q9W1C5; -.
DR PRIDE; Q9W1C5; -.
DR EnsemblMetazoa; FBtr0072168; FBpp0072077; FBgn0034964.
DR GeneID; 37840; -.
DR KEGG; dme:Dmel_CG3173; -.
DR UCSC; CG3173-RA; d. melanogaster.
DR CTD; 26173; -.
DR FlyBase; FBgn0034964; IntS1.
DR VEuPathDB; VectorBase:FBgn0034964; -.
DR eggNOG; KOG4596; Eukaryota.
DR GeneTree; ENSGT00390000015743; -.
DR HOGENOM; CLU_001690_0_0_1; -.
DR InParanoid; Q9W1C5; -.
DR OMA; NWDTIER; -.
DR OrthoDB; 357673at2759; -.
DR PhylomeDB; Q9W1C5; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; Q9W1C5; -.
DR BioGRID-ORCS; 37840; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37840; -.
DR PRO; PR:Q9W1C5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034964; Expressed in adult Malpighian tubule (Drosophila) and 26 other tissues.
DR Genevisible; Q9W1C5; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR GO; GO:0034474; P:U2 snRNA 3'-end processing; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR022145; DUF3677.
DR InterPro; IPR038902; INTS1.
DR PANTHER; PTHR21224; PTHR21224; 1.
DR Pfam; PF12432; DUF3677; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Membrane; Nucleus; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..2053
FT /note="Integrator complex subunit 1"
FT /id="PRO_0000437668"
FT TRANSMEM 708..728
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 36..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 150
FT /note="S -> T (in Ref. 3; AAN71641)"
FT /evidence="ECO:0000305"
FT CONFLICT 1539
FT /note="D -> E (in Ref. 3; AAN71641)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2053 AA; 235099 MW; 832B92FAE75EB3F3 CRC64;
MDRGKGSGSN RSQKKVPLGG ELFALGKSVR DDSKSKILPI KGMSSSDRKR EASTALASSS
KRFRGNLKDA GAPDMSSGSS QCETWEQFAV DCDLDTVVET IYAALEQNDS ETVGRLVCGV
IKQTTTSSSR SKVDNIALLA LIYVAKVQPS IFCTDIVACA LLSFLRREAN VKMRYNTNLH
ILFANLLTRG FMEISQWPEV LLRIYIDDAV NERYWADNEL CAPLVKNICA AFKTRTPHIS
LLRWDVSSSL PSGQAHRDSM TVDDDSGDNS TQSLDASPLN TESEPIPDAM CTTKSRFSDA
VVQKHVSDAI RDQLNKRQQQ DNYTRNFLKF LCTTSGIAEV RCLSISRLEL WIHNGKLVKF
AQQLLSYICF NIKGRNTQDN EVLLVLVKMR LKTKPLINHY MSCLKEMIFL QPEILSTVMK
LVVQNELSNT RNPNNMGMLA TMFQTSADQS AATLAEIYQE FLLQRDDCLR TLRVFLRELV
RMLRFDVNLV KFCKTFLSER EDLTPQIEMF EFKERIFNSM VDIVCLCMFL SATPQAREAS
LSLKTNRDTK NNHALLKLYN QMSQIQLDTV SWMYETVPTL FKIPAAEYHQ ALHKLLLLDS
PEQYSRCDQW PSEPERGAIL RIISETPIHE ETLLRIILIG ITKDIPFSIA NTFDVLLLVI
KRVSGMKATN IPAVQANKFD IIDFLFSMSE YHHPENIRLP AEYEPPKLAI IAFYWKAWLI
LLMISAHNPS SFGAFCWDHY PTMKMMMEIC ITNQFNNSSA TKDELQIITM ERDHILQFET
YLAAQTSPHA VITEETAILI TQLMLMDPMG TPRKVPSMVL DQLKFLNQTY KLGHLFCRCR
KPDLLLDIIQ RQGTTQSMPW LSDLVQNSEG DFSHLPVQCL CEFLLFNAHI INEENSRDAE
LVNFLRNLIF DGNLSHQIVC ELLDYIFRRL SSTVKQSRVA ALSGLKIIFR HSGDFENEWL
LKSLQQIPHF YEVKPFIIPQ LRAACQVENC PELIMAYIQF ITAHTLNDPV NEMLDHVIDM
AQLIVERSTM FQHIIISQED YDYVPDENRI QTLKCLFVMF NNYIIKLREY HEPYEWTEYP
DLLMVQFDDG VQLPLHINII HAFIILLTYS NSNMPESIPI LDYWFPPGRP APVAFLPSMP
QEQVQLLPDW LKLKMIRSSV DRLIEAALND LTPDQIVLFV QNFGTPVNSM SKLLAMLDTA
VLEQFDLVKN AILNKAYLAQ LIEIQQARGA KNGHYTVQAL DLHSHSQTVP DLPKISVVIQ
EAVEIDDYDS SDSDDRPTNF LATKEVAQTI LTQPDQLTES RSDCRSLIQK LLDMLASPNS
NRADVVNAIT EVLAVGCSVT MSRHACTFLR TFFSCMLHSD KYHILENALQ KNLSMFKHTF
ADSSLLQKSE LYHESLVFML RNSREIYAQQ FKANTALVAR KRIVRAIVQS FDQTKDSKTV
AKSKSDQLFH NGLFIDWLSE MDPEIVSTQL MKERFLFSKS CSEFRFYLLS LINHQTNWDT
IERIAEYLFK NFHEDYDYAT VLNYFEALTT NPKLWKGRDK YMSKNVRPDA FFMLRTSELE
PFSHFILHEG LSEVKLDSKN YDFKLCSRMN LLFKLTEKRR DLMVKVMEHV EKSSVSDYLK
LQVLQQMYIM YPRIKFLKPG KTGEQAYKLQ NLKGCQADKV SNNLITCLGS LVGKKDFETL
STDTELLLRK LAASHPLLFL RQLGVLSSIM QGRAQLSMKA LREEHHFHRF VQILRTLELL
QPTIFEEAYK NEIQNTLSCY FNFFKHHSNV KEACQMLNKF VQMLQAYINY NPSSALLFIE
QYVGILKELA AKYTSLGKLQ VLVQAVALLQ HKSHSATELD DEEVKYEYDL DEHFDVKPSA
SKPVVTEDPI EVNPQTPIDP SSSRGPLSVL TLGSYSRSNY TDISPHFLDL VKIIKQSNTE
DVVLGPMQEL ECLTSKRFVF INELFERLLN LIFSPSAQIR SIAFIILIRH LKHNPGNSDI
NLCTLNAYIQ CLRDENSSVA ATAIDNLPEM SVLLQEHAID ILTVAFSLGL KSCLNTGHQI
RKVLQTLVIQ HGY
