INT2_DROME
ID INT2_DROME Reviewed; 1105 AA.
AC Q9VX31;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Integrator complex subunit 2 {ECO:0000303|PubMed:21078872};
GN Name=IntS2 {ECO:0000312|FlyBase:FBgn0030858};
GN ORFNames=CG8211 {ECO:0000312|FlyBase:FBgn0030858};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAV36934.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAV36934.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). May mediate
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex (By similarity).
CC {ECO:0000250|UniProtKB:Q9H0H0, ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250|UniProtKB:Q9H0H0};
CC Single-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 2 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48750.2; -; Genomic_DNA.
DR EMBL; BT016049; AAV36934.1; -; mRNA.
DR RefSeq; NP_573232.1; NM_133004.2.
DR AlphaFoldDB; Q9VX31; -.
DR SMR; Q9VX31; -.
DR STRING; 7227.FBpp0074254; -.
DR PaxDb; Q9VX31; -.
DR PRIDE; Q9VX31; -.
DR DNASU; 32745; -.
DR EnsemblMetazoa; FBtr0074480; FBpp0074254; FBgn0030858.
DR GeneID; 32745; -.
DR KEGG; dme:Dmel_CG8211; -.
DR UCSC; CG8211-RA; d. melanogaster.
DR CTD; 57508; -.
DR FlyBase; FBgn0030858; IntS2.
DR VEuPathDB; VectorBase:FBgn0030858; -.
DR eggNOG; ENOG502QSP2; Eukaryota.
DR GeneTree; ENSGT00390000011888; -.
DR HOGENOM; CLU_007707_0_0_1; -.
DR InParanoid; Q9VX31; -.
DR OMA; SRTFLKH; -.
DR OrthoDB; 173270at2759; -.
DR PhylomeDB; Q9VX31; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 32745; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 32745; -.
DR PRO; PR:Q9VX31; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030858; Expressed in egg cell and 22 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR InterPro; IPR026236; Int2_metazoa.
DR InterPro; IPR029321; INTS2.
DR PANTHER; PTHR28608; PTHR28608; 1.
DR Pfam; PF14750; INTS2; 1.
DR PRINTS; PR02105; INTSUBUNIT2.
PE 1: Evidence at protein level;
KW Membrane; Nucleus; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1105
FT /note="Integrator complex subunit 2"
FT /id="PRO_0000437669"
FT TRANSMEM 822..842
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1105 AA; 124890 MW; 6FC8C810A52D9CA2 CRC64;
MPVRMYDVSP RVFCAMQNLD ITLLASYPEA EIRPVLPSLV RMSLLSPLDN TESSMESRKE
ILAVLIGIEV VNSIVSYLQV NYHELENELK KELQARQKSA FFEGQQHEYG LQSGIALGFE
RADVARKVRV VLSEIFNLQQ QVSEQKPAAH SEMLDDGIYL EEVVDILCIA LAELPSLLNI
LELTDALVHV PNGHRIICAL VANFPDCYRD VVSHVIANCD EDGSDGKHRL MLLMGLSEMN
PSQALANRSM CVDMLKVPSF MLKLTLKHPE DLIAFLTGLL LGNDQNLRSW FAAYIRSSQK
RKGDALNLVR VELLQKVIQT TTNAAELRDF NLQGAVLLRL YCALRGIGGL KFNDDEINAL
SQLVTSCPQA TPSGVRFVTL ALCMLIACPS LVSTIPLENK AVEWLQWLIR EDAFFCKRPG
TSTSLGEMLL LLAIHFHSNQ ISAISEMVCS TLAMKIPIRP NSTNRIKQLF TQDLFTEQVV
ALHAVRVPVT PNLNGTILCY LPVHCIQQLL KSRTFLKHKV PIKSWIFKQI CSSVRPVHPV
MPALVEVFVN TLIIPNPTGK VNIDHMHRPF TEAEILHVFR TSKLTFFAEE LPPMAESQEL
NQIEVTCPLT AQLLMIYYLM LYEDTRLMNL SALGGRKQKE YSNNFLGGLP LKYLLQKAHH
YHNDYLSLFH PLLRLIISNY PHLSMVDDWL EEHNLAQGNS TVVVSKHELK PETLDRALAA
IQTKPHLAIR VFKQLLQMPP ETQAQYGQQL VKHLPMVFAK SVPRYVKDLY NDIWLRLNAV
LPTTLWIMSL RAITNGSDTM DRRTFANESL LEPMEVLSCP RFVFCSPYLL MILLRILKGS
LAASKTYLNV HMQMQQKQVL DKNGMMQTDA IWEDLRTTLI ASQESAAVHI LLEVLDYIAS
KATDRVSHLE LREIQGIIGT YVHQAFISEP SLAKLVHFQT YPKSVIPMMV ASVPSMHICI
DFVHEFLNVT EMEKQIFTID LTSHLVLNYS IPKSLGVSKF CLNVIQTTLS MLTASTKCRF
LRNVMPAMVR FVETFPILAD DCVNILMTTG RILHSQSSLG MTTMEMPLTD SDKLCTYRDA
QLHIIMIEDA FKALVTAVMK KSDLY
