INT2_ECOLX
ID INT2_ECOLX Reviewed; 337 AA.
AC P62590; P09999; P11743;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Integrase/recombinase;
DE AltName: Full=E2 protein;
GN Name=int;
OS Escherichia coli.
OG Plasmid IncN R46, Plasmid IncP-beta R751, Plasmid RGN238, Plasmid R6-5,
OG Plasmid pLMO20, Plasmid pLMO27, Plasmid pLMO150, and Plasmid pLMO229.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncN R46;
RX PubMed=2821509; DOI=10.1093/nar/15.18.7491;
RA Hall R.M., Vockler C.;
RT "The region of the IncN plasmid R46 coding for resistance to beta-lactam
RT antibiotics, streptomycin/spectinomycin and sulphonamides is closely
RT related to antibiotic resistance segments found in IncW plasmids and in
RT Tn21-like transposons.";
RL Nucleic Acids Res. 15:7491-7501(1987).
RN [2]
RP PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=RGN238;
RX PubMed=2823258; DOI=10.1073/pnas.84.21.7378;
RA Ouellette M., Bissonnette L., Roy P.H.;
RT "Precise insertion of antibiotic resistance determinants into Tn21-like
RT transposons: nucleotide sequence of the OXA-1 beta-lactamase gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:7378-7382(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=RGN238;
RA Ouellette M.;
RL Submitted (DEC-1987) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pLMO20, and R6-5;
RX PubMed=3054482; DOI=10.1007/bf00339581;
RA Sundstroem L., Radstroem P., Swedberg G., Skoeld O.;
RT "Site-specific recombination promotes linkage between trimethoprim- and
RT sulfonamide resistance genes. Sequence characterization of dhfrV and sulI
RT and a recombination active locus of Tn21.";
RL Mol. Gen. Genet. 213:191-201(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncP-beta R751; TRANSPOSON=Tn5090;
RX PubMed=8195081; DOI=10.1128/jb.176.11.3257-3268.1994;
RA Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H.,
RA Sundstroem L.;
RT "Transposon Tn5090 of plasmid R751, which carries an integron, is related
RT to Tn7, Mu, and the retroelements.";
RL J. Bacteriol. 176:3257-3268(1994).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-145.
RC PLASMID=pLMO150, and pLMO229;
RX PubMed=2188588; DOI=10.1128/aac.34.4.642;
RA Sundstroem L., Skoeld O.;
RT "The dhfrI trimethoprim resistance gene of Tn7 can be found at specific
RT sites in other genetic surroundings.";
RL Antimicrob. Agents Chemother. 34:642-650(1990).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-145.
RC PLASMID=pLMO27; TRANSPOSON=Tn5086;
RA Sundstroem L., Swedberg G., Skold O.;
RL Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Putative integrase believed to be involved in the insertion
CC of antibiotic resistance genes into plasmids and transposons.
CC -!- MISCELLANEOUS: The sequence shown is that of plasmid IncN R46.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; M95287; AAB59081.1; -; Genomic_DNA.
DR EMBL; J02967; AAA91585.1; -; Genomic_DNA.
DR EMBL; X12868; CAA31355.1; -; Genomic_DNA.
DR EMBL; X12870; CAA31361.1; -; Genomic_DNA.
DR EMBL; X17477; CAA35508.1; -; Genomic_DNA.
DR EMBL; X17478; CAA35511.1; -; Genomic_DNA.
DR EMBL; X58425; CAA41325.1; -; Genomic_DNA.
DR PIR; C26839; C26839.
DR RefSeq; WP_000845048.1; NZ_WVVZ01000094.1.
DR RefSeq; YP_001096414.1; NC_009133.1.
DR RefSeq; YP_001816602.1; NC_010558.1.
DR RefSeq; YP_003108348.1; NC_013122.1.
DR RefSeq; YP_003937677.1; NC_014615.1.
DR RefSeq; YP_006903510.1; NC_019043.1.
DR RefSeq; YP_006952404.1; NC_019062.1.
DR RefSeq; YP_006953615.1; NC_019082.1.
DR RefSeq; YP_006953994.1; NC_019091.1.
DR RefSeq; YP_007349449.1; NC_020086.1.
DR RefSeq; YP_008574969.1; NC_022375.1.
DR RefSeq; YP_008864015.1; NC_022992.1.
DR RefSeq; YP_008864682.1; NC_022996.1.
DR RefSeq; YP_009060117.1; NC_024956.1.
DR RefSeq; YP_009060568.1; NC_024967.1.
DR RefSeq; YP_009061089.1; NC_024975.1.
DR RefSeq; YP_009061439.1; NC_024978.1.
DR RefSeq; YP_009061544.1; NC_024979.1.
DR RefSeq; YP_009061697.1; NC_024980.1.
DR RefSeq; YP_009068297.1; NC_025139.1.
DR RefSeq; YP_009068489.1; NC_025141.1.
DR RefSeq; YP_009068728.1; NC_025142.1.
DR RefSeq; YP_009068858.1; NC_025143.1.
DR RefSeq; YP_009069126.1; NC_025144.1.
DR RefSeq; YP_009070864.1; NC_025176.1.
DR RefSeq; YP_009071507.1; NC_025183.1.
DR RefSeq; YP_190211.1; NC_006671.1.
DR AlphaFoldDB; P62590; -.
DR SMR; P62590; -.
DR GeneID; 67375554; -.
DR OMA; LHIMLLY; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR011946; Integrase_integron-type.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR Pfam; PF13495; Phage_int_SAM_4; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR TIGRFAMs; TIGR02249; integrase_gron; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Plasmid;
KW Transposable element; Viral genome integration; Virus entry into host cell.
FT CHAIN 1..337
FT /note="Integrase/recombinase"
FT /id="PRO_0000197530"
FT DOMAIN 14..94
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 112..328
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 146
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 171
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 277
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 280
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 303
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 312
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT VARIANT 32
FT /note="R -> P (in plasmid pLMO150)"
FT VARIANT 82
FT /note="A -> P (in plasmid pLMO20)"
FT VARIANT 84
FT /note="A -> P (in plasmid pLMO20)"
FT VARIANT 205
FT /note="E -> Q (in plasmid RGN238)"
SQ SEQUENCE 337 AA; 38381 MW; D78D2A1F46752C2E CRC64;
MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE
VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD
EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML
PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST
DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL
LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER
