INT2_MOUSE
ID INT2_MOUSE Reviewed; 1198 AA.
AC Q80UK8; Q5SXZ5; Q5SXZ6; Q6PCY7; Q6ZPU6; Q8CB15; Q9CSE0;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Integrator complex subunit 2;
DE Short=Int2;
GN Name=Ints2; Synonyms=Kiaa1287;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryonic tail;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=B5/EGFP; TISSUE=Brain, and Trophoblast stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex. {ECO:0000250|UniProtKB:Q9H0H0}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.
CC {ECO:0000250|UniProtKB:Q9H0H0}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250|UniProtKB:Q9H0H0};
CC Single-pass membrane protein {ECO:0000250|UniProtKB:Q9H0H0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80UK8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80UK8-2; Sequence=VSP_018574, VSP_018575;
CC -!- SIMILARITY: Belongs to the Integrator subunit 2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98133.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK129323; BAC98133.1; ALT_INIT; mRNA.
DR EMBL; AK013101; BAB28647.1; -; mRNA.
DR EMBL; AK037055; BAC29686.1; -; mRNA.
DR EMBL; AL592065; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC050081; AAH50081.1; -; mRNA.
DR EMBL; BC059056; AAH59056.1; -; mRNA.
DR CCDS; CCDS25198.1; -. [Q80UK8-1]
DR RefSeq; NP_081697.2; NM_027421.2. [Q80UK8-1]
DR RefSeq; XP_006534242.1; XM_006534179.3. [Q80UK8-1]
DR RefSeq; XP_006534243.1; XM_006534180.2. [Q80UK8-1]
DR RefSeq; XP_006534244.1; XM_006534181.3. [Q80UK8-1]
DR RefSeq; XP_006534245.1; XM_006534182.3.
DR AlphaFoldDB; Q80UK8; -.
DR SMR; Q80UK8; -.
DR IntAct; Q80UK8; 1.
DR MINT; Q80UK8; -.
DR STRING; 10090.ENSMUSP00000018212; -.
DR iPTMnet; Q80UK8; -.
DR PhosphoSitePlus; Q80UK8; -.
DR EPD; Q80UK8; -.
DR jPOST; Q80UK8; -.
DR MaxQB; Q80UK8; -.
DR PaxDb; Q80UK8; -.
DR PeptideAtlas; Q80UK8; -.
DR PRIDE; Q80UK8; -.
DR ProteomicsDB; 269490; -. [Q80UK8-1]
DR ProteomicsDB; 269491; -. [Q80UK8-2]
DR Antibodypedia; 57004; 73 antibodies from 19 providers.
DR Ensembl; ENSMUST00000018212; ENSMUSP00000018212; ENSMUSG00000018068. [Q80UK8-1]
DR Ensembl; ENSMUST00000108039; ENSMUSP00000103674; ENSMUSG00000018068. [Q80UK8-1]
DR GeneID; 70422; -.
DR KEGG; mmu:70422; -.
DR UCSC; uc007ksi.1; mouse. [Q80UK8-1]
DR CTD; 57508; -.
DR MGI; MGI:1917672; Ints2.
DR VEuPathDB; HostDB:ENSMUSG00000018068; -.
DR eggNOG; ENOG502QSP2; Eukaryota.
DR GeneTree; ENSGT00390000011888; -.
DR HOGENOM; CLU_007707_0_0_1; -.
DR InParanoid; Q80UK8; -.
DR OMA; SRTFLKH; -.
DR PhylomeDB; Q80UK8; -.
DR TreeFam; TF324737; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 70422; 22 hits in 70 CRISPR screens.
DR ChiTaRS; Ints2; mouse.
DR PRO; PR:Q80UK8; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q80UK8; protein.
DR Bgee; ENSMUSG00000018068; Expressed in manus and 223 other tissues.
DR ExpressionAtlas; Q80UK8; baseline and differential.
DR Genevisible; Q80UK8; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC-UCL.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC-UCL.
DR InterPro; IPR026236; Int2_metazoa.
DR InterPro; IPR029321; INTS2.
DR PANTHER; PTHR28608; PTHR28608; 1.
DR Pfam; PF14750; INTS2; 1.
DR PRINTS; PR02105; INTSUBUNIT2.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..1198
FT /note="Integrator complex subunit 2"
FT /id="PRO_0000236100"
FT TRANSMEM 421..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 396..398
FT /note="PTE -> YSI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018574"
FT VAR_SEQ 399..1198
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018575"
FT CONFLICT 11
FT /note="V -> A (in Ref. 4; AAH50081)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="R -> H (in Ref. 1; BAC98133 and 4; AAH50081)"
FT /evidence="ECO:0000305"
FT CONFLICT 883
FT /note="A -> G (in Ref. 2; BAB28647)"
FT /evidence="ECO:0000305"
FT CONFLICT 887
FT /note="A -> G (in Ref. 2; BAB28647)"
FT /evidence="ECO:0000305"
FT CONFLICT 992
FT /note="C -> R (in Ref. 4; AAH50081)"
FT /evidence="ECO:0000305"
FT CONFLICT 1100
FT /note="S -> G (in Ref. 4; AAH50081)"
FT /evidence="ECO:0000305"
FT CONFLICT 1143
FT /note="R -> C (in Ref. 4; AAH50081)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1198 AA; 133515 MW; 6D140CAFC6582976 CRC64;
MTPEGTGLQF VSPFAFEAMQ KVDVVRLASL SDPELRLLLP CLVRMALCAP ADQSQSWAQD
KKLILRLLSG VEAVNSIVAL LSVDFHALEQ DASKEQQLRH KLGGGSGESI LVSQLQHGLT
LEFEHSDSPR RLRLVLSELL AIMNKVSECN GEFFFKSSEL FESAVYLEEA ADVLCILQAE
LPSLLPIVDV AEALLRVRNG AWFLCLLVAN VPDSFNEVCR GLIKNGERQD EESLGGRRRT
DALRFLCRMN PSQALKVRGM VVEECHLPGL GVALTLDHTK TEACEDGVSD LVCFVSGLLL
GTNAKVRTWF GTFIRNGQQR KRETSGSVLW QMRRQLLLEL MGILPTVRST RIVEEADVEM
EPTVSVYSGL KEEHVVKASA LLRLYCALMG IAGLKPTEEE AEQLLQLMTS RPPATPAGVR
FVSLSFCMLL AFSTLVSTPE QEQLMVLWLS WMIKEEAYFE STSGVSASFG EMLLLVAMYF
HSNQLSAIID LVCSTLGMKI VIKPSSLSRM KTIFTQEIFT EQVVTAHAVR VPVTSNLSAN
ITGFLPIHCI YQLLRSRSFT KHKVSIKDWI YRQLCETSTP LHPQLLPLID VYINSILTPA
SKSNPEATNQ PVTEQEILNL FQEVIGGDSV RLTQRFSITA QLLVLYYILS YEEALLANTK
TLASMQRKPK SYSSSLMDQI PIKFLIRQAQ GLQQELGGLH SALLRLLATN YPHLCIVDDW
ICEEEITGTD ALLRRMLLTS NAKTHSPKQL QEAFSAVPVS HTQVMQIMEH LTLLSASELI
PYAEVLTSNM NQLLNSGVPR RILQTVNKLW MVLNTVMPRR LWVMTVNALQ PSIKFIRQQK
YTQNDLMIDP LIVLRCDRRV HRCPPLMDVT LHMLNGYLLA SKAYLSAHLK ETAEQDRPSP
NNTVGLVGQT DAPEVTREEL KNALLAAQDS AAVQILLEIC LPTEEEKAKG ANSDISLRNT
QGVTTISTPS KETEEGEDNL LCNLREVQCL ICCLLHQMYI ADPNIAKLVH FQGYPCELLP
LTVAGIPSMH ICLDFIPELI AQPELEKQIF AIQLLSHLCI QYALPKSLSV ARLAVNVMGT
LLTVLTQAKR YSFFMPTLPS LVSFCRAFPP LYEDIMSLLI QIGQVCASDV ATQTRDIDPI
ITRLQQIKEK PSGWSQICKD PSYKNGSRDT GSMDPDVQLC HCIESTIIEI INMSVSGI
