ID   INT2_PSEAI              Reviewed;         337 AA.
AC   P62591; P09999; P11743;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Integrase/recombinase;
DE   AltName: Full=E2 protein;
GN   Name=int;
OS   Pseudomonas aeruginosa.
OG   Plasmid R1033, and Plasmid pVS1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=R1033; TRANSPOSON=Tn1696;
RX   PubMed=2549372; DOI=10.1007/bf02464882;
RA   Wohlleben W., Arnold W., Bissonnette L., Pelletier A., Tanguay A.,
RA   Roy P.H., Gamboa G.C., Barry G.F., Aubert E., Davies J., Kagan S.A.;
RT   "On the evolution of Tn21-like multiresistance transposons: sequence
RT   analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-
RT   I), another member of the Tn21-based expression cassette.";
RL   Mol. Gen. Genet. 217:202-208(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pVS1;
RX   PubMed=1310501; DOI=10.1128/jb.174.4.1248-1257.1992;
RA   Bissonnette L., Roy P.H.;
RT   "Characterization of In0 of Pseudomonas aeruginosa plasmid pVS1, an
RT   ancestor of integrons of multiresistance plasmids and transposons of Gram-
RT   negative bacteria.";
RL   J. Bacteriol. 174:1248-1257(1992).
CC   -!- FUNCTION: Putative integrase believed to be involved in the insertion
CC       of antibiotic resistance genes into plasmids and transposons.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; U12338; AAB59999.1; -; Genomic_DNA.
DR   EMBL; X15852; CAA33849.1; -; Genomic_DNA.
DR   EMBL; U49101; AAC44315.1; -; Genomic_DNA.
DR   EMBL; M73819; AAA25857.1; -; Genomic_DNA.
DR   PIR; A42646; A42646.
DR   PIR; JQ0300; JQ0300.
DR   RefSeq; WP_000845048.1; NZ_WXZU01000049.1.
DR   RefSeq; YP_001427371.1; NC_009739.1.
DR   RefSeq; YP_245442.1; NC_007100.1.
DR   AlphaFoldDB; P62591; -.
DR   SMR; P62591; -.
DR   GeneID; 67375554; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR011946; Integrase_integron-type.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF13495; Phage_int_SAM_4; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   TIGRFAMs; TIGR02249; integrase_gron; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element; Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..337
FT                   /note="Integrase/recombinase"
FT                   /id="PRO_0000197531"
FT   DOMAIN          14..94
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          112..328
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        146
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        171
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        280
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        312
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   VARIANT         39
FT                   /note="H -> N (in plasmid R1033)"
FT   VARIANT         187
FT                   /note="S -> T (in plasmid R1033)"
SQ   SEQUENCE   337 AA;  38381 MW;  D78D2A1F46752C2E CRC64;
     MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE
     VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD
     EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML
     PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST
     DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL
     LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER