INT3_ARATH
ID INT3_ARATH Reviewed; 580 AA.
AC Q9ZQP6; A9JNR7;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Probable inositol transporter 3;
GN Name=INT3; OrderedLocusNames=At2g35740; ORFNames=T20F21.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=16603666; DOI=10.1104/pp.106.077123;
RA Schneider S., Schneidereit A., Konrad K.R., Hajirezaei M.-R., Gramann M.,
RA Hedrich R., Sauer N.;
RT "Arabidopsis INOSITOL TRANSPORTER4 mediates high-affinity H+ symport of
RT myoinositol across the plasma membrane.";
RL Plant Physiol. 141:565-577(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=17951450; DOI=10.1104/pp.107.109033;
RA Schneider S., Schneidereit A., Udvardi P., Hammes U., Gramann M.,
RA Dietrich P., Sauer N.;
RT "Arabidopsis INOSITOL TRANSPORTER2 mediates H+ symport of different
RT inositol epimers and derivatives across the plasma membrane.";
RL Plant Physiol. 145:1395-1407(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
CC -!- FUNCTION: Plasma membrane inositol-proton symporter. {ECO:0000250,
CC ECO:0000269|PubMed:17951450}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZQP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZQP6-2; Sequence=VSP_043682, VSP_043683;
CC -!- MISCELLANEOUS: [Isoform 2]: Major isoform, probably not functional. May
CC be due to competing donor splice site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; AJ973177; CAJ00305.1; -; mRNA.
DR EMBL; AM778029; CAO82752.1; -; mRNA.
DR EMBL; AC006068; AAD15441.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09155.1; -; Genomic_DNA.
DR PIR; D84772; D84772.
DR RefSeq; NP_181117.1; NM_129132.2. [Q9ZQP6-1]
DR AlphaFoldDB; Q9ZQP6; -.
DR SMR; Q9ZQP6; -.
DR STRING; 3702.AT2G35740.1; -.
DR PaxDb; Q9ZQP6; -.
DR PRIDE; Q9ZQP6; -.
DR EnsemblPlants; AT2G35740.1; AT2G35740.1; AT2G35740. [Q9ZQP6-1]
DR GeneID; 818146; -.
DR Gramene; AT2G35740.1; AT2G35740.1; AT2G35740. [Q9ZQP6-1]
DR KEGG; ath:AT2G35740; -.
DR Araport; AT2G35740; -.
DR TAIR; locus:2058774; AT2G35740.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q9ZQP6; -.
DR OMA; WALYYTI; -.
DR OrthoDB; 326501at2759; -.
DR PhylomeDB; Q9ZQP6; -.
DR PRO; PR:Q9ZQP6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZQP6; differential.
DR Genevisible; Q9ZQP6; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005366; F:myo-inositol:proton symporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015798; P:myo-inositol transport; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 2.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..580
FT /note="Probable inositol transporter 3"
FT /id="PRO_0000259877"
FT TRANSMEM 34..54
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT VAR_SEQ 149..182
FT /note="PARIRGALVSTNGLLITGGQFLSYLINLAFVHTP -> HTGYMEVDVGSLGD
FT SSDHSVLPHANAARVSTVAL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17951450"
FT /id="VSP_043682"
FT VAR_SEQ 183..580
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17951450"
FT /id="VSP_043683"
SQ SEQUENCE 580 AA; 63172 MW; FC6437FD5A3E00B3 CRC64;
MVEEASKSEQ INITEVWTTT WETPYIMRLA LSAGIGGLLF GYNTGVIAGA LLYIKEEFGE
VDNKTWLQEI IVSMTVAGAI VGAAIGGWYN DKFGRRMSVL IADVLFLLGA LVMVIAHAPW
VIILGRLLVG FGVGMASMTS PLYISEMSPA RIRGALVSTN GLLITGGQFL SYLINLAFVH
TPGTWRWMLG VSAIPAIIQF CLMLTLPESP RWLYRNDRKA ESRDILERIY PAEMVEAEIA
ALKESVRAET ADEDIIGHTF SDKLRGALSN PVVRHGLAAG ITVQVAQQFV GINTVMYYSP
TILQFAGYAS NKTAMALALI TSGLNAVGSV VSMMFVDRYG RRKLMIISMF GIITCLVILA
AVFNEASNHA PKIDKRDSRN FAKNATCPAF APFTASRSPP SNWNCMKCLQ YDCGFCSNGA
QEYAPGACIV QSADMKALCH SKGRTFFKDG CPSKFGYLAI VFLGLYIIVY APGMGTVPWI
VNSEIYPLRY RGLAGGIAAV SNWMSNLVVS ETFLTLTNAV GSSGTFLLFA GSSAVGLFFI
WLLVPETKGL QFEEVEKLLE GGFRPSLLRP TTKENQVETP
