INT3_DANRE
ID INT3_DANRE Reviewed; 1017 AA.
AC Q1LXC9; Q58EQ4;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Integrator complex subunit 3;
DE Short=Int3;
DE AltName: Full=SOSS complex subunit A;
DE AltName: Full=Sensor of single-strand DNA complex subunit A;
DE Short=SOSS-A;
DE Short=Sensor of ssDNA subunit A;
GN Name=ints3; ORFNames=si:dkey-27c15.2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 258-1017.
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Integrator complex. The Integrator complex
CC is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription
CC and in their 3'-box-dependent processing (By similarity).
CC {ECO:0000250}.
CC -!- FUNCTION: Component of the SOSS complex, a multiprotein complex that
CC functions downstream of the MRN complex to promote DNA repair and G2/M
CC checkpoint. The SOSS complex associates with single-stranded DNA at DNA
CC lesions and influences diverse endpoints in the cellular DNA damage
CC response including cell-cycle checkpoint activation, recombinational
CC repair and maintenance of genomic stability. Required for efficient
CC homologous recombination-dependent repair of double-strand breaks
CC (DSBs). In the SOSS complex, it is required for the assembly of the
CC complex and for stabilization of the complex at DNA damage sites (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator. Component of
CC the SOSS complex, composed of soss-b (soss-b1/nabp2 or soss-b2/nabp1),
CC soss-a/ints3 and soss-c/inip. {ECO:0000250|UniProtKB:Q68E01}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q68E01}.
CC Note=Localizes to nuclear foci following DNA damage.
CC {ECO:0000250|UniProtKB:Q68E01}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 3 family. {ECO:0000305}.
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DR EMBL; BX465837; CAK10929.1; -; Genomic_DNA.
DR EMBL; BC091803; AAH91803.1; -; mRNA.
DR RefSeq; NP_001038397.1; NM_001044932.1.
DR AlphaFoldDB; Q1LXC9; -.
DR SMR; Q1LXC9; -.
DR STRING; 7955.ENSDARP00000067792; -.
DR PaxDb; Q1LXC9; -.
DR PRIDE; Q1LXC9; -.
DR Ensembl; ENSDART00000067793; ENSDARP00000067792; ENSDARG00000016811.
DR GeneID; 560575; -.
DR KEGG; dre:560575; -.
DR CTD; 65123; -.
DR ZFIN; ZDB-GENE-060503-634; ints3.
DR eggNOG; KOG4262; Eukaryota.
DR GeneTree; ENSGT00390000014184; -.
DR HOGENOM; CLU_007659_0_0_1; -.
DR InParanoid; Q1LXC9; -.
DR OMA; LCHEDNI; -.
DR OrthoDB; 644334at2759; -.
DR PhylomeDB; Q1LXC9; -.
DR TreeFam; TF323623; -.
DR Reactome; R-DRE-6807505; RNA polymerase II transcribes snRNA genes.
DR PRO; PR:Q1LXC9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 19.
DR Bgee; ENSDARG00000016811; Expressed in blastula and 27 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0070876; C:SOSS complex; ISS:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0006281; P:DNA repair; ISS:UniProtKB.
DR GO; GO:0044818; P:mitotic G2/M transition checkpoint; ISS:UniProtKB.
DR GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR InterPro; IPR045334; INTS3.
DR InterPro; IPR019333; INTS3_N.
DR PANTHER; PTHR13587; PTHR13587; 1.
DR Pfam; PF10189; Ints3_N; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..1017
FT /note="Integrator complex subunit 3"
FT /id="PRO_0000259537"
FT REGION 952..1017
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 992..1017
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 258
FT /note="L -> G (in Ref. 2; AAH91803)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1017 AA; 116403 MW; 6ACA921837D0CD62 CRC64;
MEPSPAKGKA QGRLLVSTSL DAKDELEERL ERCMSITTSI TNGLSEREAN DALTAHVCKG
PQQHEEVCLG LFTLLLTEPP QAQRCYRDLT LVNRDGMNVV LMKINQILME KFLKLQDVCR
TQLVWLVREL VKSGVIGADG VLMTLMKQIA GGDISSKNLW LAENVLDILV DQREWVLKSG
MLVAMSLYTY LRLIVDHGTT SLLPLRQREV DFCIGLLRER FMECFIIGRD LVRLLQNVAR
IPEMELVWRD LLHSPQTLSP QFTGILQLLT SRTSRKFLAC RLTPDMETKL LFMTSRVRFG
QQKRYQDWFQ RQYLSTAESQ SLRCDLIRYI CGVVHPSNEV LSSDILPRWA IIGWLLTTCT
SNVAASNAKL ALFYDWLFFN PEKDSIMNIE PAILVMHHSM KPHPAITATL LDFMCRIIPH
FFPPLEGQVR QGVFNSLNFI MEKRVLAHLA PLFDNPKLDR ELRSMLRERF PEFCSSPSPP
TEVKMEESVP LEMDNHVLDK EDGCYDNTDA TFSDDEEELN NKGKKREFRF HQLKETYIDE
PSDITPFVDQ LDEALKERVL QLQKGSDTET HCEVMQEIVD LILEEDFDSE QMSTLASCLA
ELFKSHFRGD VLPEEITEES LEESVCKPVC LIFRNLCQMQ EDNSGFSVLL DLLAELYQKQ
PKIGYHLLYY LKASKAASGK MSLYESFAQA TALGDLHTCL MMDMKACQED DVRLLCYLTP
SIYSEFPDET LRSGELLNMI VAVIDSAQLQ ELMCHVMMGN LVMFRKDSVL NILIQSLDWE
TFEQYSTWQL FLAHSIPLET IIPILQHLKY KEHPEALSCL LLQLRREKPS EEMVKMVLSR
PYHQEDQFTT SILRHWTAKH DDLLGEHIKA LLIKNNNMPR KRQSLRSSSS KLAQLTLEQM
LEHLDSLRLS LSNTKNNFFS QTPILQALQH VQASCDEAHK MRFSDLFSLA EEYEDSSKPP
KSRRKAPASS PRSRKGAAPQ PCNEEESVSS SASEEEDSKP KASKRKRKGS AVGSDSD
