INT3_DROME
ID INT3_DROME Reviewed; 1068 AA.
AC Q7PLS8; Q7PLS7; Q8IH20;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Integrator complex subunit 3 {ECO:0000303|PubMed:21078872};
DE AltName: Full=SOSS complex subunit A homolog {ECO:0000250|UniProtKB:Q68E01};
GN Name=IntS3 {ECO:0000312|FlyBase:FBgn0262117};
GN ORFNames=CG17665 {ECO:0000312|FlyBase:FBgn0262117};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1038; SER-1039; SER-1043 AND
RP SER-1044, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [6]
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [7]
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). However this
CC subunit is not essential for the 3'-end processing of the snRNA U7 and
CC the spliceosomal snRNAs U1, U2, U4 and U5 (PubMed:21078872,
CC PubMed:23097424). Mediates recruitment of cytoplasmic dynein to the
CC nuclear envelope, probably as component of the INT complex (By
CC similarity). {ECO:0000250|UniProtKB:Q68E01,
CC ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q68E01}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN71224.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAV36968.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AE013599; EAA46157.1; -; Genomic_DNA.
DR EMBL; BT001469; AAN71224.1; ALT_FRAME; mRNA.
DR EMBL; BT016083; AAV36968.1; ALT_SEQ; mRNA.
DR EMBL; BT044308; ACH92373.1; -; mRNA.
DR RefSeq; NP_001036437.1; NM_001042972.2.
DR AlphaFoldDB; Q7PLS8; -.
DR SMR; Q7PLS8; -.
DR BioGRID; 78135; 1.
DR IntAct; Q7PLS8; 1.
DR STRING; 7227.FBpp0110478; -.
DR iPTMnet; Q7PLS8; -.
DR PaxDb; Q7PLS8; -.
DR PRIDE; Q7PLS8; -.
DR EnsemblMetazoa; FBtr0111268; FBpp0110478; FBgn0262117.
DR GeneID; 3355010; -.
DR KEGG; dme:Dmel_CG17665; -.
DR UCSC; CG17665-RA; d. melanogaster.
DR UCSC; CG17665-RB; d. melanogaster.
DR CTD; 65123; -.
DR FlyBase; FBgn0262117; IntS3.
DR VEuPathDB; VectorBase:FBgn0262117; -.
DR eggNOG; KOG4262; Eukaryota.
DR GeneTree; ENSGT00390000014184; -.
DR HOGENOM; CLU_007659_0_0_1; -.
DR InParanoid; Q7PLS8; -.
DR OMA; LCHEDNI; -.
DR OrthoDB; 644334at2759; -.
DR PhylomeDB; Q7PLS8; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 3355010; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3355010; -.
DR PRO; PR:Q7PLS8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0262117; Expressed in cleaving embryo and 22 other tissues.
DR Genevisible; Q7PLS8; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR InterPro; IPR045334; INTS3.
DR InterPro; IPR019333; INTS3_N.
DR PANTHER; PTHR13587; PTHR13587; 1.
DR Pfam; PF10189; Ints3_N; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..1068
FT /note="Integrator complex subunit 3"
FT /id="PRO_0000385310"
FT REGION 916..939
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1000..1068
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1040..1068
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1038
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18327897"
FT MOD_RES 1039
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18327897"
FT MOD_RES 1043
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18327897"
FT MOD_RES 1044
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18327897"
SQ SEQUENCE 1068 AA; 123951 MW; D5EBFE8E955EB114 CRC64;
MEQQQSKNNA HVSKLFICTA VDCKDDIEEK FERSFVTLQM QISGLSDKEM HDMLSQAVCK
EKQHEEISIG FLYIMLTDPS MASKTYRDVT LVSRDGMNGI VTNLTFLVAE KYTKLTEVAR
RQLIWLLREF VKHQVLSVEN VIWNCLRQAG GGDVSSRNLF LIESLLDIFI EFRTWLETNP
FLVQSTVYSF VRLIEDHANP ALLSLRQKEV KFTISLIRER FHDIIPLGRD FVRLLQNVAR
IPEFEQLWRD ILFNPKMLHQ TFNGIWQLLH IRTSRRFLQC RLLPEMERKI SFLASSVKFG
NQKRYQDWFQ DKYFATPESH SLRSDLIRFI INVIHPTNDM LCSDIIPRWA IIGWLISSCT
NPIASANAKL SLFYDWLFFD PAKDNIMNIE PGILVMYHSI RNHPFVSSTL LDFLCRITKN
FFVKHEDKIR IGVYNSLKLI LEKQVIPNLQ PLFESPKLDR ELRNLIRDNF REFLSPPANH
GQLLFTSLHP VQGHILKKES DQRILHCENM DLHETGLINI SGTIDEDKKI LLVPTDQEIE
SVFSDETAEN LRRVHNIEDN TDDDDDLPLS EVRLKEKPKV ELAEAIAESF DAFVTKRNSY
TWEAFLKDFR PLPASAFEEF QLNYVISNTV LILRETLPQQ NIFSESKTEE KHLAKSISYP
LYGLFRFLYE NDEKSKKPFQ TLLSEICEGI PEIGYLLLYF MKIYCKLQTR KNSQQSYQFK
TTIYRQICDA ADEKIGNCLL RDLDLLEKEN TNIFLWLLPD IYREFKSIAT NNTDLLRITL
RCIDAKNVRD ILYSIAQGKL TIFKQDGLID CIRQSLEFET YEQFCLWQIV QAHDVPLRCI
QDILPELEAG SHPEALSHFL LLLKNEEPTN EIIRLMLSRE SKSKGDPFVT SALRFWCQRY
EEKLSEIIAS LLTSKYPSSS PNKRKRPPKG ISVSTSTPSA DQVLNHLEHY RRSCRHGTGT
GLYVHDMMQR ALQSAYSHSN DSTKKQFCDL FALAAEEDTT VGRRGGSGRG RKQPGSKKDV
NNHGTSKKNP EMVKTIYSSD DNSSEEDWSK SKILQTAKRR KKANNDSD
