INT4_DROME
ID INT4_DROME Reviewed; 1022 AA.
AC Q9W3E1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Integrator complex subunit 4 {ECO:0000303|PubMed:21078872};
GN Name=IntS4 {ECO:0000312|FlyBase:FBgn0026679};
GN Synonyms=l(1)G0095 {ECO:0000312|FlyBase:FBgn0026679};
GN ORFNames=CG12113 {ECO:0000312|FlyBase:FBgn0026679};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [4] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). Essential for
CC development (PubMed:21078872). May mediate recruitment of cytoplasmic
CC dynein to the nuclear envelope, probably as component of the INT
CC complex (By similarity). {ECO:0000250|UniProtKB:Q96HW7,
CC ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 4 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF46388.1; -; Genomic_DNA.
DR EMBL; AY051460; AAK92884.1; -; mRNA.
DR RefSeq; NP_572488.1; NM_132260.3.
DR AlphaFoldDB; Q9W3E1; -.
DR SMR; Q9W3E1; -.
DR IntAct; Q9W3E1; 6.
DR STRING; 7227.FBpp0071178; -.
DR PaxDb; Q9W3E1; -.
DR PRIDE; Q9W3E1; -.
DR EnsemblMetazoa; FBtr0071234; FBpp0071178; FBgn0026679.
DR GeneID; 31793; -.
DR KEGG; dme:Dmel_CG12113; -.
DR UCSC; CG12113-RA; d. melanogaster.
DR CTD; 92105; -.
DR FlyBase; FBgn0026679; IntS4.
DR VEuPathDB; VectorBase:FBgn0026679; -.
DR eggNOG; KOG2259; Eukaryota.
DR GeneTree; ENSGT00390000010128; -.
DR HOGENOM; CLU_012910_1_0_1; -.
DR InParanoid; Q9W3E1; -.
DR OMA; PHMQGSF; -.
DR OrthoDB; 256550at2759; -.
DR PhylomeDB; Q9W3E1; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; Q9W3E1; -.
DR BioGRID-ORCS; 31793; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 31793; -.
DR PRO; PR:Q9W3E1; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0026679; Expressed in cleaving embryo and 44 other tissues.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; IMP:FlyBase.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1022
FT /note="Integrator complex subunit 4"
FT /id="PRO_0000437657"
FT REGION 818..840
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 818..837
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1022 AA; 113423 MW; 8D434B2F2A21D389 CRC64;
MALAIKKRVG TYVETVDGSP PVKKLRLQTL AADAKGGKSG KVGNVERKLT ALNQLDAYVG
NLPAGALVLP TGTPVASTGA PSTGVIGNPP AAATGAPPMT AANSRELLEL LVKITDEISY
EDVEMGELKE VASKIFQLYQ LQERDSDTSI RVKLLELLSG LGCECATEQA LTMIIDYFIF
LLRKEVSQKV LAQGMMCLFR IGERRKHMLP ISYKTQVAHL AKEQLRSGSA HTQKNAMLVI
GRFATKMEGE RHYVWKLAFY IDSQDSSVRA QALHALLTLG ERGSQLPAVL YKRAVEAMKD
DYECVRKEAL QLVFMLGNRH PDYILPSDRQ QEELRMIDAA FSKVCEALCD LSLQIRVLAA
ELLGGMTAVS REFLHQTLDK KLMSNLRRKR TAHERGARLV ASGEWSSGKR WADDAPQEHL
DAQSISIIAS GACGALIHGL EDEFLEVRTA AVASMCKLAL SRPDFAVTSL DFLVDMFNDE
IEDVRLKAIY SLTAIAKHIV LREDQLEIML GSLEDYSVDV REGLHLMLGA CRVSTQTCLL
MVVQKLLDVL AKYPQDRNST YACMRKIGQK HPHLVMAVAV HLLYVHPFFE TPERDVEDPA
YLCVLILVFN AAEHLVPIIS LLPTATHRHY AYLRDSMPNL VPQLPIEGAS SASATHRIDS
AMHQAGSSAE YLQMILSHIE EIFTMTDERL ELLQTAQSNL QRLGSIDAGM YGTSNFLETF
LAAQIQIEQM QRCASTQRSR VPLKESLAAL IRNCLKLQHT FSGLNYGDIL QVKQLRLRAC
ALHLVLVVRD RSQSALGPCQ MLLQTAGDIS EFIKANTKDE EEKPPVVETD MPMKESVSRD
AQPDSFTRQL LIKLDGISDP KPGRVFREIL PLVQQAPPLA LPPANDKIRR CVANILEPCP
LQSQDNVIKV TAGLIAAVPF VAEIDNLLES QKADMRIKIK YPDQHMHTVV PKQSDFKPIM
TEQGEHKTNV RLRTTILLSH SVWTESSLVE IQLCLAVRPG SELELCKPAK VLFAPKPVRR
GI
