INT5_DROME
ID INT5_DROME Reviewed; 994 AA.
AC Q9VFS6; Q95R54;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Integrator complex subunit 5 {ECO:0000303|PubMed:21078872};
DE AltName: Full=Protein oocyte maintenance defects {ECO:0000312|FlyBase:FBgn0038168};
GN Name=omd {ECO:0000312|FlyBase:FBgn0038168};
GN Synonyms=IntS5 {ECO:0000303|PubMed:21078872};
GN ORFNames=CG9591 {ECO:0000312|FlyBase:FBgn0038168};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAR96207.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAR96207.1};
RC TISSUE=Testis {ECO:0000312|EMBL:AAR96207.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AAL29156.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 605-994.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL29156.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL29156.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, AND IDENTIFICATION IN THE INTEGRATOR COMPLEX.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872). May mediate recruitment of
CC cytoplasmic dynein to the nuclear envelope, probably as component of
CC the INT complex (By similarity). {ECO:0000250|UniProtKB:Q6P9B9,
CC ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000269|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}. Nucleus {ECO:0000250|UniProtKB:Q6P9B9}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q6P9B9}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL29156.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF54973.2; -; Genomic_DNA.
DR EMBL; AE014297; AGB95924.1; -; Genomic_DNA.
DR EMBL; AE014297; AGB95925.1; -; Genomic_DNA.
DR EMBL; BT011415; AAR96207.1; -; mRNA.
DR EMBL; AY061608; AAL29156.1; ALT_INIT; mRNA.
DR RefSeq; NP_001262542.1; NM_001275613.1.
DR RefSeq; NP_001262543.1; NM_001275614.1.
DR RefSeq; NP_650303.1; NM_142046.2.
DR AlphaFoldDB; Q9VFS6; -.
DR SMR; Q9VFS6; -.
DR IntAct; Q9VFS6; 1.
DR STRING; 7227.FBpp0082313; -.
DR PaxDb; Q9VFS6; -.
DR PRIDE; Q9VFS6; -.
DR DNASU; 41674; -.
DR EnsemblMetazoa; FBtr0082848; FBpp0082313; FBgn0038168.
DR EnsemblMetazoa; FBtr0334530; FBpp0306597; FBgn0038168.
DR EnsemblMetazoa; FBtr0334531; FBpp0306598; FBgn0038168.
DR GeneID; 41674; -.
DR KEGG; dme:Dmel_CG9591; -.
DR UCSC; CG9591-RA; d. melanogaster.
DR CTD; 4958; -.
DR FlyBase; FBgn0038168; omd.
DR VEuPathDB; VectorBase:FBgn0038168; -.
DR eggNOG; ENOG502QPVK; Eukaryota.
DR GeneTree; ENSGT00390000008374; -.
DR HOGENOM; CLU_013732_0_0_1; -.
DR InParanoid; Q9VFS6; -.
DR OMA; KDFCVHS; -.
DR OrthoDB; 108880at2759; -.
DR PhylomeDB; Q9VFS6; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 41674; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 41674; -.
DR PRO; PR:Q9VFS6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038168; Expressed in eye disc (Drosophila) and 25 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR InterPro; IPR040316; INTS5.
DR InterPro; IPR029444; INTS5_C.
DR InterPro; IPR029445; INTS5_N.
DR PANTHER; PTHR31697; PTHR31697; 1.
DR Pfam; PF14838; INTS5_C; 2.
DR Pfam; PF14837; INTS5_N; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..994
FT /note="Integrator complex subunit 5"
FT /id="PRO_0000437748"
FT TRANSMEM 769..786
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 810..826
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 559..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 570..586
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 994 AA; 112972 MW; 9B3DAB184E4B8296 CRC64;
MLRQNLLDQL KHFIETVSNG HSCPQLLTSP NLIKLALGFL EELPATRDIV FEYFALLAEI
SVQLYVSPEM ADPKTGMPVS QVKLAGNRQQ QQRAPEYEAF NLVKTALQSL VWKGPPAWSP
LIANWSLELV AKLSDKYTQR RMTITASCNY WLECSAMHGL MTLINSCFRK LTQPEEEACV
EIMLNAFHRF PMTFDWIVAR LGGCFPYKII MQILQCGIKR FVDDYRCHLD SEAGILDYMT
SCHEQHLRAA FREMLREGFA PKKPLDVAVV PFLLITTNYS DTILQSLVNV LVEIYTEDMC
EVIVQKAPLW LSNKMFAGMQ PTLNNAVLRL NERGATLLLT AAKMAEKYVW CQDFLDNSMQ
ELEQWVLNQR NFPLLADLAY EETKYMLWKS CLSTNLFEQQ TAVRLLLVVS SQHPNIYYQT
ISQLLKKSYA QNPNGIGALI RLLGGQSGMV NFPGFTPGFK MVLEDITLDV QVNNRLPVPP
GTPTEAFNTF SNLNILARMH KSKNVAPYIK AQHLNQALNE CLPKILQIFD CTVNKLVLRI
DRDAAERIAD KFRAQQSKNS NNNNELCNGK DYGKRTKLEP GEDKVDDEDA TRMRLAHLIV
DLLNNIEAGS RTTVLRTPLV LKLATLSVKY FFVGLTEKTV IRRAAASHRS YTLLQRQCSA
RKIARTVCLR ELVERALFYH GHLLGQLEVY QLDELEIPEH EHLILQNLHT SSGANSNRSV
LHSGIIGRGL RPVLPPSERN CDAEKQALYL KALNACCADL EKPNNVEGYS LVSLLLVELV
STDVMYNGLP FPDEEFTRVT MERDMLIRRA FINSPVLWAV LGLIAGHRPA LCYSSVLLRA
LCATCLHHWR GKNVNRFQPT AANDELMLCT KKMLQLLAMS QLIPPPLTNL HLIIEHFESA
EIALLLRECI WNYLKDHVPS PALFHVDNNG LHWRNTNTQL AKVPPQYVDP LRHLMQRKLS
TLGPHYHQMF IMGELMEGDS EPDPTARLQI VEID
