INT5_MOUSE
ID INT5_MOUSE Reviewed; 1018 AA.
AC Q8CHT3; Q3TM44;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Integrator complex subunit 5;
DE Short=Int5;
GN Name=Ints5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Heart, and Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex. {ECO:0000250|UniProtKB:Q6P9B9}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.
CC {ECO:0000250|UniProtKB:Q6P9B9}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}. Nucleus {ECO:0000250|UniProtKB:Q6P9B9}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q6P9B9}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 5 family. {ECO:0000305}.
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DR EMBL; AK155499; BAE33294.1; -; mRNA.
DR EMBL; AK166149; BAE38598.1; -; mRNA.
DR EMBL; AK168795; BAE40628.1; -; mRNA.
DR EMBL; AK170584; BAE41894.1; -; mRNA.
DR EMBL; BC039214; AAH39214.1; -; mRNA.
DR CCDS; CCDS29556.1; -.
DR RefSeq; NP_789813.1; NM_176843.3.
DR AlphaFoldDB; Q8CHT3; -.
DR SMR; Q8CHT3; -.
DR BioGRID; 224544; 5.
DR STRING; 10090.ENSMUSP00000093968; -.
DR iPTMnet; Q8CHT3; -.
DR PhosphoSitePlus; Q8CHT3; -.
DR EPD; Q8CHT3; -.
DR MaxQB; Q8CHT3; -.
DR PaxDb; Q8CHT3; -.
DR PeptideAtlas; Q8CHT3; -.
DR PRIDE; Q8CHT3; -.
DR ProteomicsDB; 269069; -.
DR Antibodypedia; 28628; 100 antibodies from 21 providers.
DR DNASU; 109077; -.
DR Ensembl; ENSMUST00000096249; ENSMUSP00000093968; ENSMUSG00000071652.
DR GeneID; 109077; -.
DR KEGG; mmu:109077; -.
DR UCSC; uc008gnu.2; mouse.
DR CTD; 80789; -.
DR MGI; MGI:1923578; Ints5.
DR VEuPathDB; HostDB:ENSMUSG00000071652; -.
DR eggNOG; ENOG502QPVK; Eukaryota.
DR GeneTree; ENSGT00390000008374; -.
DR HOGENOM; CLU_013732_0_0_1; -.
DR InParanoid; Q8CHT3; -.
DR OMA; KDFCVHS; -.
DR OrthoDB; 108880at2759; -.
DR PhylomeDB; Q8CHT3; -.
DR TreeFam; TF323720; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 109077; 23 hits in 72 CRISPR screens.
DR ChiTaRS; Ints5; mouse.
DR PRO; PR:Q8CHT3; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q8CHT3; protein.
DR Bgee; ENSMUSG00000071652; Expressed in dorsal pancreas and 259 other tissues.
DR Genevisible; Q8CHT3; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC-UCL.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC-UCL.
DR InterPro; IPR040316; INTS5.
DR InterPro; IPR029444; INTS5_C.
DR InterPro; IPR029445; INTS5_N.
DR PANTHER; PTHR31697; PTHR31697; 1.
DR Pfam; PF14838; INTS5_C; 1.
DR Pfam; PF14837; INTS5_N; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q6P9B9"
FT CHAIN 2..1018
FT /note="Integrator complex subunit 5"
FT /id="PRO_0000259542"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 855..875
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 929..949
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..21
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P9B9"
FT MOD_RES 278
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P9B9"
FT CONFLICT 276
FT /note="P -> H (in Ref. 1; BAE38598)"
FT /evidence="ECO:0000305"
FT CONFLICT 648
FT /note="R -> K (in Ref. 1; BAE38598)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1018 AA; 108348 MW; 6B6C8E0B41F206CD CRC64;
MSALCDPPGA PGPPGPAPAT HGPAPLSAQE LSQEIKAFLT GVDPILGHQL SAREHARCGL
LLLRSLPPAR AAVLDHLRGV FDESVRAHLA ALEESPVAGP PHLRPPPPSH VPTGGPGLED
VVHEVQQVLC EFIRANPKAW APVISAWSID LMGQLSSTYS GQHQRVPHAT GSLNELLQLW
MGCRATRTLM DIYVQCLSAL IGSCPDACVD ALLDTSVQHS PHFDWVVAHI GSSFPGTIIS
RVLSCGLKDF CVHSGAGGGA SACGNSSQPP STDPFPGSPA IPGEKRVPKI ASVVGILGHL
ASRHGDSIRR ELLRMFHDSL AGGSGGRNGE PSLQATVPFL LQLAVMSPAL LGTVSGELVD
CLKPPAVLSQ LQQHLQGFPR EELDNMLNLA VHLVSQASGT GAYRLLQFLV DTAMPASVIT
TQGLAVPDTV REACDRLIQL LLLHLQKLVH HRGGSPGEGV LGPPPPPRPV PFLDALRNHV
GELCGETLRL ERKRFLWQHQ LLGLLSVYTR PSCGPEALGH LLSRARSPEE LSLATQLYAG
LVVSLSGLLP LAFRSCLARV HAGTLQPPFT ARFLRNLALL VGWEQQGGEG PSALGARFGE
SASAHLADLA PLLLHPEEEV AEAAASLLAI CPFPSEALSP SQLLGLVRAG VHHFFSSLRL
HGPPGVASAS QLLTRLSQTS PAGLKAVLQL LVEGALHRGN TELFGGEMDG DNETLSIVST
PLASASLLDI NRRHTAAVPG PGGIWSVFHA GVIGRGLKPP KIVQSRNHQE VIYNTQSLVS
LLVHCCSASG NSEREGCWGA PTLSPEAAKA VAVTLVESVC PDAAGAELAW PPEDHARATV
ERDLRIGRRF REQPLLFELL KLVAAAPPAL CYCSVLLRGL LAALLSHWEA SRHPDTTHSP
WHLEASCILV AVMAEGSLLP PALGNMHEVF SQLAPFEVRL LLLSVWGFLR EHGPLPQKFI
FQSERGRFIR DFAREGGAEG GPHLSVLHSV LHRNIDRLGL FSGRFQAPPP STLLRQGT
