INT6_DROME
ID INT6_DROME Reviewed; 1284 AA.
AC Q9W485; Q960F1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Integrator complex subunit 6 {ECO:0000303|PubMed:21078872};
GN Name=IntS6 {ECO:0000312|FlyBase:FBgn0261383};
GN Synonyms=l(1)G0060 {ECO:0000312|FlyBase:FBgn0261383};
GN ORFNames=CG3125 {ECO:0000312|FlyBase:FBgn0261383};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93517.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93517.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAK93517.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Farfan D., Frise E., George R., Gonzalez M.,
RA Guarin H., Li P., Liao G., Miranda A., Mungall C.J., Nunoo J., Pacleb J.,
RA Paragas V., Park S., Phouanenavong S., Wan K., Yu C., Lewis S.E.,
RA Rubin G.M., Celniker S.;
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). May mediate
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex (By similarity).
CC {ECO:0000250|UniProtKB:Q9UL03, ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 6 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF46073.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09149.1; -; Genomic_DNA.
DR EMBL; AE014298; AGB95105.1; -; Genomic_DNA.
DR EMBL; AE014298; AGB95106.1; -; Genomic_DNA.
DR EMBL; AE014298; AGB95107.1; -; Genomic_DNA.
DR EMBL; AY052093; AAK93517.1; -; mRNA.
DR RefSeq; NP_001259259.1; NM_001272330.1.
DR RefSeq; NP_001259260.1; NM_001272331.1.
DR RefSeq; NP_001259261.1; NM_001272332.1.
DR RefSeq; NP_572253.2; NM_132025.3.
DR RefSeq; NP_727026.1; NM_167047.2.
DR AlphaFoldDB; Q9W485; -.
DR SMR; Q9W485; -.
DR IntAct; Q9W485; 2.
DR STRING; 7227.FBpp0070806; -.
DR PaxDb; Q9W485; -.
DR PRIDE; Q9W485; -.
DR EnsemblMetazoa; FBtr0070841; FBpp0070806; FBgn0261383.
DR EnsemblMetazoa; FBtr0070842; FBpp0070807; FBgn0261383.
DR EnsemblMetazoa; FBtr0332399; FBpp0304673; FBgn0261383.
DR EnsemblMetazoa; FBtr0332400; FBpp0304674; FBgn0261383.
DR EnsemblMetazoa; FBtr0332401; FBpp0304675; FBgn0261383.
DR GeneID; 31496; -.
DR KEGG; dme:Dmel_CG3125; -.
DR UCSC; CG3125-RA; d. melanogaster.
DR CTD; 26512; -.
DR FlyBase; FBgn0261383; IntS6.
DR VEuPathDB; VectorBase:FBgn0261383; -.
DR eggNOG; KOG3768; Eukaryota.
DR GeneTree; ENSGT00390000016655; -.
DR HOGENOM; CLU_006789_0_0_1; -.
DR InParanoid; Q9W485; -.
DR OMA; QLKHIKN; -.
DR OrthoDB; 551849at2759; -.
DR PhylomeDB; Q9W485; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; Q9W485; -.
DR BioGRID-ORCS; 31496; 1 hit in 3 CRISPR screens.
DR ChiTaRS; IntS6; fly.
DR GenomeRNAi; 31496; -.
DR PRO; PR:Q9W485; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0261383; Expressed in spermathecum and 23 other tissues.
DR ExpressionAtlas; Q9W485; baseline and differential.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR029307; INT_SG_DDX_CT_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF15300; INT_SG_DDX_CT_C; 1.
DR Pfam; PF13519; VWA_2; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1284
FT /note="Integrator complex subunit 6"
FT /id="PRO_0000437658"
FT DOMAIN 3..134
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 653..824
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 864..895
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1053..1086
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1125..1180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 678..692
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 750..784
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 796..824
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..895
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1125..1177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 265
FT /note="S -> P (in Ref. 3; AAK93517)"
FT /evidence="ECO:0000305"
FT CONFLICT 279
FT /note="Q -> H (in Ref. 3; AAK93517)"
FT /evidence="ECO:0000305"
FT CONFLICT 762
FT /note="S -> L (in Ref. 3; AAK93517)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1284 AA; 137108 MW; 585C82896EE07574 CRC64;
MTIILFLVDT SSSMCQKAYV NGVQKTYLDI AKGAVETFLK YRQRTQDCLG DRYMLLTFEE
PPANVKAGWK ENHATFMNEL KNLQSHGLTS MGESLRNAFD LLNLNRMQSG IDTYGQGRCP
FYLEPSVIIV ITDGGRYSYR NGVHQEIILP LSNQIPGTKF TKEPFRWDQR LFSLVLRMPG
NKIDERVDGK VPHDDSPIER MCEVTGGRSY RVRSHYVLNQ CIESLVQKVQ PGVVLQFEPM
LPKEATSATA GEAAGASTIS GMGISSTSGA GPAPDIVFQP VKKMIYVQKH ITQKTFPIGY
WPLPEPYWPD SKAITLPPRD AHPKLKVLTP AVDEPQLVRS FPVDKYEIEG CPLTLQILNK
REMNKCWQVI VTNGMHGFEL PFGYLKAAPN FSQVHLYVLA YNYPALLPIL HDLIHKYNMS
PPSDLMYKFN AYVRSIPPYY CPFLRKALVN INVPYQLLQF LLPENVDNYL SPTIANQLKH
IKNTAKQDQE NLCMKVYKQL KQPKPPYRQV ETGKLFTGAP LRRDLVRHPL LRDIFSKLHG
EIDPVENYTI VVPQPTHQSS AKSHRNPFDI PRRDLVEEIA KMRETLLRPV SLVAKDSGHC
LPIAEMGNYQ EYLKNKDNPL REIEPTNVRQ HMFGNPYKKD KHMVMVDEAD LSDVAPMKSP
NGNGPGGAPP GSPSGSGSPT GPGPSSPGSS PGGGSGPGMP GMPGMGGGMS GLMLGAGGSG
GSSKKLDGTS RGRKRKAGPL PRSFEFRRSS TDSRSSSSGS ESSTTGSAPG SPIPGATSSI
SGCDSAMGAD GGPSSSCFDE DSNSNSSFVS STSEASASDS GMSNSHLDPL RISFVFIEEE
TSDQDTPLNG YVHNFINGIS DDAETGETEA VPGGASLPGA SSANEPSSIG ASPAVSATPA
TSVIPASNGS EVCSAVAAGS SSSISSSASS PGVLYVPLNG LTTHAAYSSN LGGPSSPLDN
LSSLGGAAGG GGSGLLGVSK PGSLPLANGY GHGHSSAISP PSPLSLVPLS PLGTSTPAAS
SSGLSSSSAY FSHNDINDAS EISRILQTCH NGTSSGSSVG AGASNSNLNG NGSTESGGGV
SSDDHASLGG NSFDALKRTA GTAGSTAAGN PHYYWASGLN HHNNNNSSAA GAASGSTLSN
NHNHRGHNNS SPNKLEVKIN SSCGSSPTHN NGGMGSPGQS LPLIFTEEQR EAARLHNVEL
RLQIFRDIRR PGRDYSQLLE HLNLVKGDQD MQSDFVDMCI VESLRFRRHR MASSIQEWWD
RKQQLTAVGT NEATPSAEQA VAKS
