INT6_MOUSE
ID INT6_MOUSE Reviewed; 883 AA.
AC Q6PCM2; Q3TA02; Q61204; Q6PDL7; Q8BQZ7;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Integrator complex subunit 6;
DE Short=Int6;
DE AltName: Full=DBI-1;
DE AltName: Full=Protein DDX26;
GN Name=Ints6; Synonyms=Dbi1, Ddx26;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 312-883 (ISOFORM 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Corpora quadrigemina, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=9473344; DOI=10.1006/excr.1997.3865;
RA Hoff H.B. III, Tresini M., Li S., Sell C.;
RT "DBI-1, a novel gene related to the notch family, modulates mitogenic
RT response to insulin-like growth factor 1.";
RL Exp. Cell Res. 238:359-370(1998).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex. {ECO:0000250|UniProtKB:Q9UL03}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.
CC {ECO:0000250|UniProtKB:Q9UL03}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UL03}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6PCM2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PCM2-2; Sequence=VSP_021461, VSP_021462;
CC Name=3;
CC IsoId=Q6PCM2-3; Sequence=VSP_021459, VSP_021460;
CC -!- SIMILARITY: Belongs to the Integrator subunit 6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB01338.1; Type=Miscellaneous discrepancy; Note=Chimera.; Evidence={ECO:0000305};
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DR EMBL; AK046055; BAC32584.1; -; mRNA.
DR EMBL; AK172178; BAE42868.1; -; mRNA.
DR EMBL; BC058637; AAH58637.1; -; mRNA.
DR EMBL; BC059263; AAH59263.1; -; mRNA.
DR EMBL; U57368; AAB01338.1; ALT_SEQ; mRNA.
DR CCDS; CCDS27191.1; -. [Q6PCM2-1]
DR PIR; T30176; T30176.
DR RefSeq; NP_032741.2; NM_008715.2. [Q6PCM2-1]
DR RefSeq; XP_006518745.1; XM_006518682.3. [Q6PCM2-2]
DR RefSeq; XP_011243288.1; XM_011244986.2. [Q6PCM2-1]
DR RefSeq; XP_017171398.1; XM_017315909.1. [Q6PCM2-2]
DR AlphaFoldDB; Q6PCM2; -.
DR SMR; Q6PCM2; -.
DR BioGRID; 201810; 8.
DR IntAct; Q6PCM2; 1.
DR MINT; Q6PCM2; -.
DR STRING; 10090.ENSMUSP00000086788; -.
DR iPTMnet; Q6PCM2; -.
DR PhosphoSitePlus; Q6PCM2; -.
DR EPD; Q6PCM2; -.
DR MaxQB; Q6PCM2; -.
DR PaxDb; Q6PCM2; -.
DR PeptideAtlas; Q6PCM2; -.
DR PRIDE; Q6PCM2; -.
DR ProteomicsDB; 269071; -. [Q6PCM2-1]
DR ProteomicsDB; 269072; -. [Q6PCM2-2]
DR ProteomicsDB; 269073; -. [Q6PCM2-3]
DR Antibodypedia; 730; 153 antibodies from 26 providers.
DR DNASU; 18130; -.
DR Ensembl; ENSMUST00000053959; ENSMUSP00000086788; ENSMUSG00000035161. [Q6PCM2-1]
DR Ensembl; ENSMUST00000223585; ENSMUSP00000152954; ENSMUSG00000035161. [Q6PCM2-1]
DR GeneID; 18130; -.
DR KEGG; mmu:18130; -.
DR UCSC; uc007ugp.2; mouse. [Q6PCM2-1]
DR UCSC; uc007ugr.1; mouse. [Q6PCM2-2]
DR UCSC; uc007ugu.1; mouse. [Q6PCM2-3]
DR CTD; 26512; -.
DR MGI; MGI:1202397; Ints6.
DR VEuPathDB; HostDB:ENSMUSG00000035161; -.
DR eggNOG; KOG3768; Eukaryota.
DR GeneTree; ENSGT00390000016655; -.
DR HOGENOM; CLU_006789_0_0_1; -.
DR InParanoid; Q6PCM2; -.
DR OMA; QDFTLPM; -.
DR OrthoDB; 551849at2759; -.
DR PhylomeDB; Q6PCM2; -.
DR TreeFam; TF323386; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 18130; 16 hits in 71 CRISPR screens.
DR ChiTaRS; Ints6; mouse.
DR PRO; PR:Q6PCM2; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q6PCM2; protein.
DR Bgee; ENSMUSG00000035161; Expressed in spermatocyte and 239 other tissues.
DR GO; GO:0015629; C:actin cytoskeleton; ISO:MGI.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR029307; INT_SG_DDX_CT_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF15300; INT_SG_DDX_CT_C; 1.
DR Pfam; PF13519; VWA_2; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..883
FT /note="Integrator complex subunit 6"
FT /id="PRO_0000259544"
FT DOMAIN 3..227
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 666..686
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 800
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UL03"
FT VAR_SEQ 206..219
FT /note="RSYSVCSPRMLNQC -> KVFAKSNSGLKQFI (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021459"
FT VAR_SEQ 220..883
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021460"
FT VAR_SEQ 246
FT /note="E -> EA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021461"
FT VAR_SEQ 691..700
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021462"
FT CONFLICT 64
FT /note="A -> G (in Ref. 1; BAE42868)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="A -> G (in Ref. 1; BAE42868)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="E -> G (in Ref. 1; BAE42868)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 883 AA; 99661 MW; C6364701690B8B84 CRC64;
MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY
AIKAGWKENH ATFMNELKNL QAEGLTTLGQ SLRTAFDLLN LNRLVTGIDN YGQGRNPFFL
EPAIIITITD GSKLTTTSGV QDELHLPLNS PLPGSELTKE PFRWDQRLFA LVLRLPGTMS
VESEQLTGVP LDDSAITPMC EVTGGRSYSV CSPRMLNQCL ESLVQKVQSG VVINFEKAGP
DPPPAEEGQP DISRPFGSQP WHSCHKLIYV RPNPKTGVPI GHWPVPESFW PDQNSPTLPP
RTSHPVVKFS CTDCEPMVID KLPFDKYELE PSPLTQFILE RKSPQTCWQV YVSNSAKYNE
LGHPFGYLKA STALTCVNLF VMPYNYPVLL PLLDDLFKVH KAKPTLKWRQ SFESYLKTMP
PYYLGPLKKA VRMMGAPNLI ADSMEYGLSY SVISYLKKLS QQAKIESDRV IGSVGKKVVQ
ETGIKVRSRS HGLSMAHRKG FQVLQGISED VPHRLLDLNM KEYTGFQVAL LNKDLKPQTF
RNAYDIPRRN LLDHLTRMRS NLLKSTRKFL KGQDEDQVHS VPIAQMGNYQ EYLKQVPSPL
RELDPDQPRR LHTFGNPFKL DKKGMMIDEA DEFVAGPQNK HKRPGEPSMQ GIPKRRRCAS
PLLRGRRQSP AVNSHIGGKG PPAPMTQAQP GLIKPLPLHK EATNDSIVDD VVENHVADQL
SSDMTPNAMD TEFLTSPPNL LEPSTNHTEA LGHEHLGNND LTVGGFLENH EEPRNKEQSA
EENIPASSLN KGKKLMHCRS HEEVNTELKA QIMKEIRKPG RKYERIFTLL KHVQGSLQTR
LIFLQNVIKE ASRFKKRMLI EQLENFLDEI HRRANQINHI NSN
