INT7_DROME
ID INT7_DROME Reviewed; 1001 AA.
AC Q9VT41; Q961P9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Integrator complex subunit 7 {ECO:0000303|PubMed:19326441};
DE AltName: Full=Protein deflated {ECO:0000303|PubMed:19326441};
GN Name=defl {ECO:0000312|FlyBase:FBgn0036038};
GN Synonyms=IntS7 {ECO:0000303|PubMed:19326441};
GN ORFNames=CG18176 {ECO:0000312|FlyBase:FBgn0036038};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK92871.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK92871.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAK92871.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ACH92266.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19326441; DOI=10.1002/dvdy.21922;
RA Rutkowski R.J., Warren W.D.;
RT "Phenotypic analysis of deflated/Ints7 function in Drosophila
RT development.";
RL Dev. Dyn. 238:1131-1139(2009).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [7] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). Essential for
CC development (PubMed:19326441, PubMed:21078872). Required for cell
CC signaling and/or cell proliferation (PubMed:19326441).
CC {ECO:0000269|PubMed:19326441, ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19326441}. Cytoplasm
CC {ECO:0000269|PubMed:19326441}. Note=Predominantly in the nucleus.
CC {ECO:0000269|PubMed:19326441}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels. First detected after
CC cellularization, in the early epidermal tissues of embryos undergoing
CC gastrulation. At later stages of embryogenesis, strongest expression is
CC in the gut, and there is also expression in the central nervous system
CC and perhaps the peripheral nervous system.
CC {ECO:0000269|PubMed:19326441}.
CC -!- MISCELLANEOUS: The name 'deflated' originates from the 'deflated
CC balloon' appearance of mutant abdomens. {ECO:0000303|PubMed:19326441}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 7 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50214.1; -; Genomic_DNA.
DR EMBL; AY051447; AAK92871.1; -; mRNA.
DR EMBL; BT044201; ACH92266.1; -; mRNA.
DR RefSeq; NP_648352.2; NM_140095.4.
DR AlphaFoldDB; Q9VT41; -.
DR SMR; Q9VT41; -.
DR STRING; 7227.FBpp0076132; -.
DR PaxDb; Q9VT41; -.
DR PRIDE; Q9VT41; -.
DR DNASU; 39141; -.
DR EnsemblMetazoa; FBtr0076403; FBpp0076132; FBgn0036038.
DR GeneID; 39141; -.
DR KEGG; dme:Dmel_CG18176; -.
DR UCSC; CG18176-RA; d. melanogaster.
DR CTD; 39141; -.
DR FlyBase; FBgn0036038; defl.
DR VEuPathDB; VectorBase:FBgn0036038; -.
DR eggNOG; KOG1988; Eukaryota.
DR GeneTree; ENSGT00390000011724; -.
DR HOGENOM; CLU_013157_0_0_1; -.
DR InParanoid; Q9VT41; -.
DR OMA; FAHIIES; -.
DR OrthoDB; 1131967at2759; -.
DR PhylomeDB; Q9VT41; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 39141; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 39141; -.
DR PRO; PR:Q9VT41; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036038; Expressed in embryonic/larval gut (Drosophila) and 12 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; IMP:FlyBase.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033060; INTS7.
DR PANTHER; PTHR13322; PTHR13322; 2.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..1001
FT /note="Integrator complex subunit 7"
FT /id="PRO_0000437659"
FT REGION 980..1001
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 985..1001
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 490
FT /note="K -> E (in Ref. 3; AAK92871)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1001 AA; 112288 MW; 790EA79BA7B76791 CRC64;
MSHLTGTRVS TFNESFLNEN EHDSNAVLME LDKGLRSTKQ GIQCEAVVRF PRLFEKYPFP
ILINSSFIKL ADYFVSGSNL LRFWVLRVCQ QSENHLDKIL NIDSFVRCIF VVMHSNDPVA
RALLLRTLGA VSRVIPEKQQ VHHAIRRALD SHDTVEVEAA IYASSCFAAQ SSSFAISMCA
KISDMIESLQ VPVPMKLLLI PVLRHMHHEA TTASLVSRLC MDLLPKYPAQ SFVVAIIDTL
TQLSSRTLVG VPGQLDVLLD FMQDLRTPVR IQVLRSFNEL AGRQSVHAWP KPAIKALIDR
FELCTNSKEQ FLFLSILLKL SECPLTCQQL LREHRVALLR LCIQCISKLD DYTTATQAMA
VLSVLVAFGL KKKGSGEQVD DILHMVNLHM EGLLLCTAKR SECTRDLRRV LTYGIRITKA
NAEFGTSFIG IVTNSLGDKG AYPPANAELM CEALAGLCEH FQLRKYAFST AEDLIVDENA
MDTDELPPPK INPMLARLPL ILHKLNTIID QENCDQQLRS VEILSSLVLQ TTMGCYLPQK
VVQCFEKCLG RLNCWTLYRI ARTASRYGHH YVAAHIYTKV SQIVISDHMH YFLVALSQIS
QAECILNYGL EYAYMRDNYA PKVAPEPLIP LMKRLEMASN LYQQALASLR AGSSPQHPCT
FQLEYLKIRA QFLQTLHLAV TVKNAQVIVP PPAIAGSLAQ NSRDYLQKFG HVTNQLRKLV
KALKACEETY ARLYKSSFDA DHVTLEFLEV AEFQCALFAH IIESICYATP PEPPVFLTTG
DHPETRYFAA SCQRMEQMQK NLPQEPANAK TISNRHLDVI IAQIEIITKT PLCLPRYFFQ
ILQSTQIKLS VSPQPRSATE PVNVQSGSNL VIKVEGVLQH FSKQKKHFRR VESVQLSLTS
QLITPPPRSS QELPKQGAND TVTLNQIVKP QRDFLSGSFL LPISNGGHFQ VTLETFVVDE
NGITWCTGPK SSMVVRVLED PSKQGAPAPS TSQAVGQTRR F
