INT7_MOUSE
ID INT7_MOUSE Reviewed; 966 AA.
AC Q7TQK1; Q8C3A9; Q8C3N4; Q8C3N6;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Integrator complex subunit 7;
DE Short=Int7;
GN Name=Ints7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Head, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Limb;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role
CC in DNA damage response (DDR) signaling during the S phase. May be not
CC involved in the recruitment of cytoplasmic dynein to the nuclear
CC envelope by different components of the INT complex.
CC {ECO:0000250|UniProtKB:Q9NVH2}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12. Interacts with NABP2.
CC {ECO:0000250|UniProtKB:Q9NVH2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NVH2}.
CC Chromosome {ECO:0000250|UniProtKB:Q9NVH2}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9NVH2}. Note=Localizes to sites of DNA damage
CC in a H2AX-independent manner. {ECO:0000250|UniProtKB:Q9NVH2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TQK1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TQK1-2; Sequence=VSP_021465, VSP_021466;
CC -!- SIMILARITY: Belongs to the Integrator subunit 7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC39426.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK085336; BAC39426.1; ALT_SEQ; mRNA.
DR EMBL; AK085362; BAC39430.1; -; mRNA.
DR EMBL; AK086462; BAC39671.1; -; mRNA.
DR EMBL; BC054120; AAH54120.1; -; mRNA.
DR CCDS; CCDS35825.1; -. [Q7TQK1-1]
DR RefSeq; NP_001293132.1; NM_001306203.1.
DR RefSeq; NP_001293133.1; NM_001306204.1.
DR RefSeq; NP_848747.4; NM_178632.6.
DR AlphaFoldDB; Q7TQK1; -.
DR SMR; Q7TQK1; -.
DR BioGRID; 218493; 6.
DR IntAct; Q7TQK1; 1.
DR MINT; Q7TQK1; -.
DR STRING; 10090.ENSMUSP00000036277; -.
DR iPTMnet; Q7TQK1; -.
DR PhosphoSitePlus; Q7TQK1; -.
DR EPD; Q7TQK1; -.
DR jPOST; Q7TQK1; -.
DR MaxQB; Q7TQK1; -.
DR PaxDb; Q7TQK1; -.
DR PeptideAtlas; Q7TQK1; -.
DR PRIDE; Q7TQK1; -.
DR ProteomicsDB; 301653; -. [Q7TQK1-1]
DR ProteomicsDB; 301654; -. [Q7TQK1-2]
DR DNASU; 77065; -.
DR GeneID; 77065; -.
DR KEGG; mmu:77065; -.
DR UCSC; uc007eco.1; mouse. [Q7TQK1-2]
DR UCSC; uc007ecp.1; mouse. [Q7TQK1-1]
DR CTD; 25896; -.
DR MGI; MGI:1924315; Ints7.
DR eggNOG; KOG1988; Eukaryota.
DR InParanoid; Q7TQK1; -.
DR OrthoDB; 1131967at2759; -.
DR PhylomeDB; Q7TQK1; -.
DR TreeFam; TF106105; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 77065; 24 hits in 76 CRISPR screens.
DR ChiTaRS; Ints7; mouse.
DR PRO; PR:Q7TQK1; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q7TQK1; protein.
DR GO; GO:0005694; C:chromosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; ISO:MGI.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; ISO:MGI.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033060; INTS7.
DR PANTHER; PTHR13322; PTHR13322; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Cytoplasm; DNA damage; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..966
FT /note="Integrator complex subunit 7"
FT /id="PRO_0000259550"
FT REGION 941..966
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 338
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVH2"
FT MOD_RES 809
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVH2"
FT VAR_SEQ 492..498
FT /note="SLATVIF -> RNCFFLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021465"
FT VAR_SEQ 499..966
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021466"
FT CONFLICT 870
FT /note="I -> L (in Ref. 1; BAC39426)"
FT /evidence="ECO:0000305"
FT CONFLICT 954
FT /note="L -> Q (in Ref. 1; BAC39426/BAC39671)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 966 AA; 106861 MW; 6A5D6DFC30E6447B CRC64;
MASNSTKSFL ADAGYGEQEL DANSALMELD KGLRSGKLGE QCEAVVRFPR LFQKYPFPIL
INSAFLKLAD VFRVGNNFLR LCVLKVTQQS EKHLEKILNV DEFVKRVFSV IHSNDPVARA
ITLRMLGSLA SIIPERKNAH HSIRQSLDSH DNVEVEAAVF AAANFSAQSK DFAVGICNKI
SEMIQGLATP VDLKLKLIPI LQHMHHDALL ASSARQLLQQ LVTSYPSTKM VIVSLHTFTL
LAASSLVDTP KQIQLLLQYL KNDPRKAVKR LAVQDLKLLA SKTPHTWSKE NIQALCECAL
HTPYDSLKLG MLSVLSTLSG TIAIKHYFSV VPGNVGSSPR SSDLVKLAQE CCYHSNRGIA
AHGVRVLTNI TVSCQEKDLL SLEQDAVFGL ESLLVLCSQD DSPGAQSTVK SALSCMVKLA
KGRPHLSRSV VDTLLTQLHS SQDAARILMC HCLAAIAMQL PVLGDGMLGD LVELYKVIGR
SATDKQQELL VSLATVIFVA SQKALSAEVK AVIKQQLESV SSGWTVYRIA RQASRMGNHD
MARELYQSLL TQVASEHFYF WLNSLKEFSH AEQCLTGLQE DSFSSALSCI AESLKFYHKG
IASLTAASTP LNPLSFQCEF VKLRIDLLQA FSQLICTCNS LKTSPPPAIA TTIAMTLGND
LQRCGRISNQ MKQSMEEFRS LASRYRDLYQ ASFDADSATL RNVELQQQSC LLIAHAIEAL
VLDPESASFQ EYGSTGAAHA DSEYERRMMS VYSRVLEEVE SLNRKYAPVS YMHTACLCNA
IIALLKVPLS FQRYFFQKLQ STSIKLALSP SPRSPAEPIA VQNNQQLALK VEGVVQHGSK
PGLFRRVQSV CLNVSSTLQS KSGQDYKIPI DSMTNEMEQR VEPHNDYFST QFLLNFAVLG
THSITVESSV RDANGIVWKT GPRTTMFVKS LEDPYSQQIR LQQQAQQPLQ PQPLPQPQPR
SAYTRF
