INT8_DROME
ID INT8_DROME Reviewed; 1007 AA.
AC A1ZAK1; O96831; Q95U02;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Integrator complex subunit 8 {ECO:0000303|PubMed:21078872};
GN Name=IntS8 {ECO:0000312|FlyBase:FBgn0025830};
GN ORFNames=CG5859 {ECO:0000312|FlyBase:FBgn0025830};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:CAA21313.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4] {ECO:0000312|EMBL:AAL13635.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL13635.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAL13635.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000312|EMBL:ACH92502.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [7] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424).
CC {ECO:0000269|PubMed:21078872, ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
CC {ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 8 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57945.1; -; Genomic_DNA.
DR EMBL; BT044437; ACH92502.1; -; mRNA.
DR EMBL; AL031863; CAA21313.1; -; Genomic_DNA.
DR EMBL; AY058406; AAL13635.1; -; mRNA.
DR PIR; T13693; T13693.
DR RefSeq; NP_611162.2; NM_137318.3.
DR AlphaFoldDB; A1ZAK1; -.
DR SMR; A1ZAK1; -.
DR IntAct; A1ZAK1; 4.
DR STRING; 7227.FBpp0086186; -.
DR PaxDb; A1ZAK1; -.
DR EnsemblMetazoa; FBtr0087036; FBpp0086186; FBgn0025830.
DR GeneID; 36886; -.
DR KEGG; dme:Dmel_CG5859; -.
DR UCSC; CG5859-RA; d. melanogaster.
DR CTD; 55656; -.
DR FlyBase; FBgn0025830; IntS8.
DR VEuPathDB; VectorBase:FBgn0025830; -.
DR eggNOG; ENOG502QQS8; Eukaryota.
DR GeneTree; ENSGT00390000007597; -.
DR HOGENOM; CLU_012129_0_0_1; -.
DR InParanoid; A1ZAK1; -.
DR OMA; YYSCIWD; -.
DR OrthoDB; 461829at2759; -.
DR PhylomeDB; A1ZAK1; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 36886; 0 hits in 1 CRISPR screen.
DR ChiTaRS; IntS8; fly.
DR GenomeRNAi; 36886; -.
DR PRO; PR:A1ZAK1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0025830; Expressed in oviduct (Drosophila) and 23 other tissues.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:FlyBase.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR InterPro; IPR038751; Int8.
DR PANTHER; PTHR13350; PTHR13350; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1007
FT /note="Integrator complex subunit 8"
FT /id="PRO_0000437660"
FT REGION 56..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 87
FT /note="G -> R (in Ref. 5; AAL13635)"
FT /evidence="ECO:0000305"
FT CONFLICT 371
FT /note="M -> L (in Ref. 3; CAA21313)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1007 AA; 113480 MW; 175E2577719BC983 CRC64;
MDDPLKPKPV PLAAETVLWF EFLLDPHKIT QHLQRPHPEP SAMELIVQFI SMTPNTAQES
VGTPGSDLQN LNQTPSNSGP IPGVVGGAPA PTTPTASGGV GMPHSPQRPA EKGLQLNRKQ
LALKILELKV ATWLKWDLDA LEKNLPVIMQ LALLRDLCTI SYGCSLSIPL PNDFDARISA
AGNERAARFA LTIYHRMLLR MQLIKEQALK APRPQNTMYQ TVDQLQQFLD TPTQPSIEYL
QQLCASTKPF YIFHYDSFVP LRCDDIGNGQ NYDVMHLITP QELRAQLHYE LAQYYLYTKQ
YVLAREAAAA CNTNLQAIPP QTTLYYCHIR PSELEGLLQA CGISAEEQSL LEKFQQSLLN
NYTDIVSILR MDNRRREIPF ISRRQVELDI EGSISTGILK ETVQLQLQVA ALNVVRNIFE
WGSIFGSVEY FEKYRELDCL PPLVEALQEM LPHCTFKEQA ALKHFLIDCL LHQGGQSRQL
LQTVRGFGLF SSDELQDIDE QMLQATPPVP TNSLASLSDW MCHSKMSRVD VGALERQLIS
CTNANTVRIL LVKLCATAPG KPLWAINPSW DVPQPLKTLI MAMPVSFLQD FSYVLLGKAR
ELATRGNYID AVSMLSVLKS ENQRQEMAAN VQLMCKLITW EILHIQITQC LEEWHQKPLD
LQSLGGRCKQ CLGALQAGDS IVPRPDILES CAIMLLNLTE FPPLLYLDKR AGPLELPLAF
AATFIEMEKM KGPKKVCRDA WELMLSMFLN VPKRGSSGVG GISSLQAFLQ RIRHQSVFGL
AISMIGKVHN ILKDDPNHDL SCEYMQLWPT SINNPVSYSL RSVCETLQWL LSEALSYYPQ
TISWLKMKGD LDLAIGNNES AMRCYVNALV TGTDYCTMPL QRNVADDYVI RKMIRCAANL
GCHMQATVLC QFLDEIDYGI VFKNLSEKSS NFTDAMDAYY SCIWDTTLLE FIVNLHAKRG
EHSRKLEAIS MMGTLELNAN NNEEIKRESA MVRKSRFLRA LAKQYLL
