INT8_MOUSE
ID INT8_MOUSE Reviewed; 995 AA.
AC Q80V86; A2API4; Q3TEM3; Q3TNW0; Q6PFD3; Q810I3; Q8BQJ8; Q8BT91;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Integrator complex subunit 8;
DE Short=Int8;
GN Name=Ints8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, Lung, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=28542170; DOI=10.1371/journal.pgen.1006809;
RA Oegema R., Baillat D., Schot R., van Unen L.M., Brooks A., Kia S.K.,
RA Hoogeboom A.J.M., Xia Z., Li W., Cesaroni M., Lequin M.H.,
RA van Slegtenhorst M., Dobyns W.B., de Coo I.F.M., Verheijen F.W., Kremer A.,
RA van der Spek P.J., Heijsman D., Wagner E.J., Fornerod M., Mancini G.M.S.;
RT "Human mutations in integrator complex subunits link transcriptome
RT integrity to brain development.";
RL PLoS Genet. 13:e1006809-e1006809(2017).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-
CC box-dependent processing. The Integrator complex is associated with the
CC C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A)
CC and is recruited to the U1 and U2 snRNAs genes (By similarity).
CC {ECO:0000250|UniProtKB:Q75QN2}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.
CC {ECO:0000250|UniProtKB:Q75QN2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q75QN2}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the brain at 14.5 dpc
CC especially in the cortex ventricular zone and hindbrain.
CC {ECO:0000269|PubMed:28542170}.
CC -!- SIMILARITY: Belongs to the Integrator subunit 8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH50192.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH57618.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC25374.2; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK049513; BAC33786.1; -; mRNA.
DR EMBL; AK012736; BAC25374.2; ALT_FRAME; mRNA.
DR EMBL; AK140829; BAE24491.1; -; mRNA.
DR EMBL; AK164951; BAE37977.1; -; mRNA.
DR EMBL; AK169552; BAE41225.1; -; mRNA.
DR EMBL; AL840625; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC049092; AAH49092.1; -; mRNA.
DR EMBL; BC050192; AAH50192.1; ALT_INIT; mRNA.
DR EMBL; BC057618; AAH57618.1; ALT_INIT; mRNA.
DR CCDS; CCDS17967.1; -.
DR RefSeq; NP_001153067.1; NM_001159595.1.
DR RefSeq; NP_835213.3; NM_178112.5.
DR AlphaFoldDB; Q80V86; -.
DR SMR; Q80V86; -.
DR BioGRID; 215494; 1.
DR IntAct; Q80V86; 1.
DR STRING; 10090.ENSMUSP00000038418; -.
DR iPTMnet; Q80V86; -.
DR PhosphoSitePlus; Q80V86; -.
DR EPD; Q80V86; -.
DR MaxQB; Q80V86; -.
DR PaxDb; Q80V86; -.
DR PeptideAtlas; Q80V86; -.
DR PRIDE; Q80V86; -.
DR ProteomicsDB; 266996; -.
DR Antibodypedia; 25892; 47 antibodies from 17 providers.
DR Ensembl; ENSMUST00000044616; ENSMUSP00000038418; ENSMUSG00000040738.
DR Ensembl; ENSMUST00000108319; ENSMUSP00000103955; ENSMUSG00000040738.
DR GeneID; 72656; -.
DR KEGG; mmu:72656; -.
DR UCSC; uc008rzg.2; mouse.
DR CTD; 55656; -.
DR MGI; MGI:1919906; Ints8.
DR VEuPathDB; HostDB:ENSMUSG00000040738; -.
DR eggNOG; ENOG502QQS8; Eukaryota.
DR GeneTree; ENSGT00390000007597; -.
DR HOGENOM; CLU_012129_0_0_1; -.
DR InParanoid; Q80V86; -.
DR OMA; YYSCIWD; -.
DR OrthoDB; 461829at2759; -.
DR PhylomeDB; Q80V86; -.
DR TreeFam; TF324241; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 72656; 19 hits in 75 CRISPR screens.
DR ChiTaRS; Ints8; mouse.
DR PRO; PR:Q80V86; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q80V86; protein.
DR Bgee; ENSMUSG00000040738; Expressed in embryonic post-anal tail and 258 other tissues.
DR ExpressionAtlas; Q80V86; baseline and differential.
DR Genevisible; Q80V86; MM.
DR GO; GO:0032039; C:integrator complex; ISS:HGNC-UCL.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISS:HGNC-UCL.
DR InterPro; IPR038751; Int8.
DR PANTHER; PTHR13350; PTHR13350; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..995
FT /note="Integrator complex subunit 8"
FT /id="PRO_0000259554"
FT REPEAT 250..288
FT /note="TPR 1"
FT REPEAT 320..356
FT /note="TPR 2"
FT REPEAT 570..603
FT /note="TPR 3"
FT REPEAT 833..866
FT /note="TPR 4"
FT MOD_RES 18
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q75QN2"
FT CONFLICT 40
FT /note="K -> R (in Ref. 1; BAC33786)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="K -> Q (in Ref. 1; BAC25374)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="F -> L (in Ref. 3; AAH50192)"
FT /evidence="ECO:0000305"
FT CONFLICT 600
FT /note="V -> M (in Ref. 1; BAE41225)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 995 AA; 113353 MW; 021BB8C81316899A CRC64;
MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK ACPDPAPVQL IVQFLEQASK
PSVNEQNQVQ PPPDNKRNRV LKLLALKVAA HLKWDLDILE KSLSVPVLNM LLNELLCISK
VPPGTKHVDM DLSALPPTTA MAILLYNRWA IRTIVQSSFP VKQAKPGPPQ LNVMNQMQQE
KELTENILKV LKEQAADCIL VLEAALRLNK DLYVHTMRTL DLLAVEPGTV NGETENSTAG
LKIRTEEMQC QVCYDLGAAY FQQGSTDSAI YENAREKFFR TKELLAEIGS LSLHCTIDEK
RLAGYCQACD VLVPSSDNNS QQLTPYSQVH ICLRSGSYQE VVQIFIEDNL TFTLPVQFRQ
SVLRELFQKA QQGNEALDEI CFKICVCNTV RDVLEGRTIS VQFNQLFLRP NKEKIDFLLE
VCSRSINVEK ASDSLKGNMA AFLKNVCLGL EDLQYVFMIS SHELFITLLK DEERKLLVDQ
MRKRSPRVNL CIKPVTSFYD IPASASVNIG QLEHQLILSV DPWRIRQILI ELHGMTSERQ
FWTVSNKWEV PSVYSSVILG IKDNLTRDLV YILMAKGLHC STVKDFPHSK QLFTACLELV
TEFSPKLRQV MLNEMLLLDI RTHEAGVGQS GERPPSDLIS RVRGYLEMRL PDIPLRQVVA
EECVAFMLNW RENEYLTLQV PAFLFQNNPY VKLGQLLAAT CKELPGPKES RRTAKDLWEV
VVQICSVSNQ HKRGNDGRVS LIKQRESTLG IMYRSELLSF IKKLREPLVL TIILSLFVKL
HNVREDIVND ITAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHAWLIIQA
DIYFATNQHS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCSL LNCHTQVAIL
CQFLREIDYK TAFKSLQEQN SHDAMDSYYD YIWDVTILEY LTYLHHKRGE TDKRQIAIKA
IGQTELNASN PEEVLQLAAQ RRKKKFLQAM AKLYF
