INT9_DROME
ID INT9_DROME Reviewed; 654 AA.
AC Q95TS5; Q9VUY5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Integrator complex subunit 9 {ECO:0000303|PubMed:21078872};
GN Name=IntS9 {ECO:0000312|FlyBase:FBgn0036570};
GN ORFNames=CG5222 {ECO:0000312|FlyBase:FBgn0036570};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL13803.1};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL13803.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL13803.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL13803.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=21078872; DOI=10.1128/mcb.00943-10;
RA Ezzeddine N., Chen J., Waltenspiel B., Burch B., Albrecht T., Zhuo M.,
RA Warren W.D., Marzluff W.F., Wagner E.J.;
RT "A subset of Drosophila integrator proteins is essential for efficient U7
RT snRNA and spliceosomal snRNA 3'-end formation.";
RL Mol. Cell. Biol. 31:328-341(2011).
RN [5] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND INTERACTION WITH INTS1 AND INTS12.
RX PubMed=23288851; DOI=10.1074/jbc.m112.425892;
RA Chen J., Waltenspiel B., Warren W.D., Wagner E.J.;
RT "Functional analysis of the integrator subunit 12 identifies a microdomain
RT that mediates activation of the Drosophila integrator complex.";
RL J. Biol. Chem. 288:4867-4877(2013).
RN [6] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=23097424; DOI=10.1261/rna.035725.112;
RA Chen J., Ezzeddine N., Waltenspiel B., Albrecht T.R., Warren W.D.,
RA Marzluff W.F., Wagner E.J.;
RT "An RNAi screen identifies additional members of the Drosophila Integrator
RT complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.";
RL RNA 18:2148-2156(2012).
CC -!- FUNCTION: Component of the Integrator complex, a complex involved in
CC the transcription of small nuclear RNAs (snRNA) and their 3'-box-
CC dependent processing (PubMed:21078872, PubMed:23097424). Involved in
CC the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs
CC U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). May mediate
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex (By similarity).
CC {ECO:0000250|UniProtKB:Q9NV88, ECO:0000269|PubMed:21078872,
CC ECO:0000269|PubMed:23097424}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7,
CC IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14
CC (PubMed:23097424). Within the complex, interacts with IntS1 and IntS12
CC (PubMed:23288851). {ECO:0000269|PubMed:23288851,
CC ECO:0000305|PubMed:23097424}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23288851}.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-
CC metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.
CC {ECO:0000305}.
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DR EMBL; AE014296; AAF49538.2; -; Genomic_DNA.
DR EMBL; AY058574; AAL13803.1; -; mRNA.
DR RefSeq; NP_648838.3; NM_140581.4.
DR AlphaFoldDB; Q95TS5; -.
DR SMR; Q95TS5; -.
DR IntAct; Q95TS5; 1.
DR STRING; 7227.FBpp0075250; -.
DR PaxDb; Q95TS5; -.
DR DNASU; 39763; -.
DR EnsemblMetazoa; FBtr0075495; FBpp0075250; FBgn0036570.
DR GeneID; 39763; -.
DR KEGG; dme:Dmel_CG5222; -.
DR UCSC; CG5222-RA; d. melanogaster.
DR CTD; 55756; -.
DR FlyBase; FBgn0036570; IntS9.
DR VEuPathDB; VectorBase:FBgn0036570; -.
DR eggNOG; KOG1138; Eukaryota.
DR GeneTree; ENSGT00390000001445; -.
DR HOGENOM; CLU_023159_1_0_1; -.
DR InParanoid; Q95TS5; -.
DR OMA; CADSIKD; -.
DR OrthoDB; 617493at2759; -.
DR PhylomeDB; Q95TS5; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 39763; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39763; -.
DR PRO; PR:Q95TS5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036570; Expressed in ovary and 13 other tissues.
DR ExpressionAtlas; Q95TS5; baseline and differential.
DR GO; GO:0032039; C:integrator complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IDA:FlyBase.
DR GO; GO:0016180; P:snRNA processing; ISS:FlyBase.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR022712; Beta_Casp.
DR InterPro; IPR027074; Integrator_9su.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR PANTHER; PTHR46094; PTHR46094; 1.
DR Pfam; PF10996; Beta-Casp; 1.
DR Pfam; PF16661; Lactamase_B_6; 1.
DR SMART; SM01027; Beta-Casp; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..654
FT /note="Integrator complex subunit 9"
FT /id="PRO_0000437661"
SQ SEQUENCE 654 AA; 73272 MW; 07D69E09BE590240 CRC64;
MRLYCLSGDL AKPCYIITFK GLRIMLDCGL TEQTVLNFLP LPFVQSLKWS NLPNFVPSRD
HDPQMDGELK DCCGRVFVDS TPEFNLPMDK MLDFSEVDVI LISNYLNMLA LPYITENTGF
KGKVYATEPT LQIGRFFLEE LVDYIEVSPK ACTARLWKEK LHLLPSPLSE AFRAKKWRTI
FSLKDVQGSL SKVTIMGYDE KLDILGAFIA TPVSSGYCLG SSNWVLSTAH EKICYVSGSS
TLTTHPRPIN QSALKHADVL IMTGLTQAPT VNPDTKLGEL CMNVALTIRN NGSALIPCYP
SGVVYDLFEC LTQNLENAGL NNVPMFFISP VADSSLAYSN ILAEWLSSAK QNKVYLPDDP
FPHAFYLRNN KLKHYNHVFS EGFSKDFRQP CVVFCGHPSL RFGDAVHFIE MWGNNPNNSI
IFTEPDFPYL QVLAPFQPLA MKAFYCPIDT SLNYQQANKL IKELKPNVLV IPEAYTKPHP
SAPNLFIEQP DKKIITFKCG EIIRLPLKRK LDRIYITSEL AQKISPKEVA AGVTFSTLTG
VLQVKDKVHC IQPCADSVKD ETISSNSAPT KEDVLKNVKY EYGSIDVDAV MKKLAQDGFS
NIKLDRTGGA LTLNLVNEDT VIKFEDNETH IICGGKPTTR LKLRDTIMKC LQSF
