INT9_MOUSE
ID INT9_MOUSE Reviewed; 658 AA.
AC Q8K114; Q8BP62; Q8CAE4;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Integrator complex subunit 9;
DE Short=Int9;
GN Name=Ints9; Synonyms=D14Ertd231e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Embryo, and Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP INTERACTION WITH ESRRB.
RX PubMed=26206133; DOI=10.1038/ncomms8776;
RA Latos P.A., Goncalves A., Oxley D., Mohammed H., Turro E., Hemberger M.;
RT "Fgf and Esrrb integrate epigenetic and transcriptional networks that
RT regulate self-renewal of trophoblast stem cells.";
RL Nat. Commun. 6:7776-7776(2015).
CC -!- FUNCTION: Component of the Integrator (INT) complex, a complex involved
CC in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their
CC 3'-box-dependent processing. The Integrator complex is associated with
CC the C-terminal domain (CTD) of RNA polymerase II largest subunit
CC (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates
CC recruitment of cytoplasmic dynein to the nuclear envelope, probably as
CC component of the INT complex. {ECO:0000250|UniProtKB:Q9NV88}.
CC -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8,
CC INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12 (By similarity). Interacts
CC with ESRRB, ESRRB is probably not a core component of the multiprotein
CC complex Integrator and this association is a bridge for the interaction
CC with the multiprotein complex Integrator; attracts the transcriptional
CC machinery (PubMed:26206133). {ECO:0000250|UniProtKB:Q9NV88,
CC ECO:0000269|PubMed:26206133}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NV88}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K114-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K114-2; Sequence=VSP_021470, VSP_021471;
CC -!- MISCELLANEOUS: Although strongly related to RNA-specific endonuclease
CC proteins, it lacks the HXHXDH motif that binds zinc and participates in
CC the catalytic center. Its function as endonuclease is therefore unsure.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-
CC metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC36917.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK038979; BAC30191.1; -; mRNA.
DR EMBL; AK077634; BAC36917.1; ALT_INIT; mRNA.
DR EMBL; AK136992; BAE23199.1; -; mRNA.
DR EMBL; AK150195; BAE29371.1; -; mRNA.
DR EMBL; BC028953; AAH28953.1; -; mRNA.
DR EMBL; BC055700; AAH55700.1; -; mRNA.
DR CCDS; CCDS27210.1; -. [Q8K114-1]
DR RefSeq; NP_001240660.1; NM_001253731.1.
DR RefSeq; NP_700463.2; NM_153414.4. [Q8K114-1]
DR AlphaFoldDB; Q8K114; -.
DR SMR; Q8K114; -.
DR BioGRID; 229188; 3.
DR STRING; 10090.ENSMUSP00000045552; -.
DR iPTMnet; Q8K114; -.
DR PhosphoSitePlus; Q8K114; -.
DR EPD; Q8K114; -.
DR MaxQB; Q8K114; -.
DR PaxDb; Q8K114; -.
DR PeptideAtlas; Q8K114; -.
DR PRIDE; Q8K114; -.
DR ProteomicsDB; 301655; -. [Q8K114-1]
DR ProteomicsDB; 301656; -. [Q8K114-2]
DR Antibodypedia; 23131; 140 antibodies from 24 providers.
DR DNASU; 210925; -.
DR Ensembl; ENSMUST00000043914; ENSMUSP00000045552; ENSMUSG00000021975. [Q8K114-1]
DR GeneID; 210925; -.
DR KEGG; mmu:210925; -.
DR UCSC; uc007uiv.1; mouse. [Q8K114-2]
DR UCSC; uc007uiw.2; mouse. [Q8K114-1]
DR CTD; 55756; -.
DR MGI; MGI:1098533; Ints9.
DR VEuPathDB; HostDB:ENSMUSG00000021975; -.
DR eggNOG; KOG1138; Eukaryota.
DR GeneTree; ENSGT00390000001445; -.
DR InParanoid; Q8K114; -.
DR OMA; CADSIKD; -.
DR OrthoDB; 617493at2759; -.
DR PhylomeDB; Q8K114; -.
DR TreeFam; TF314100; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 210925; 26 hits in 78 CRISPR screens.
DR ChiTaRS; Ints9; mouse.
DR PRO; PR:Q8K114; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8K114; protein.
DR Bgee; ENSMUSG00000021975; Expressed in primary oocyte and 254 other tissues.
DR ExpressionAtlas; Q8K114; baseline and differential.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0032039; C:integrator complex; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0034472; P:snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016180; P:snRNA processing; ISO:MGI.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR022712; Beta_Casp.
DR InterPro; IPR027074; Integrator_9su.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR PANTHER; PTHR46094; PTHR46094; 1.
DR Pfam; PF16661; Lactamase_B_6; 1.
DR SMART; SM01027; Beta-Casp; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isopeptide bond; Nucleus; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..658
FT /note="Integrator complex subunit 9"
FT /id="PRO_0000259559"
FT REGION 548..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 58
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NV88"
FT VAR_SEQ 522..576
FT /note="LADSLVPMEIKPGISLATVSAVLHTKDNKHVLQPPPKPTQPTSSKKRKRVNE
FT DIP -> VSCCPFTLKSLLSTRVHSGHCQSLKTEGRKEHRPPGKLCYVPYVAFTGMALQ
FT VTS (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_021470"
FT VAR_SEQ 577..658
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_021471"
SQ SEQUENCE 658 AA; 74078 MW; 617C25FA07B60F92 CRC64;
MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG
NAFLDKELKE CSGHVFVDSV PEFCLPETEL IDLSTVDVIL ISNYHCMMAL PYITEHTGFT
GTVYATEPTM QIGRLLMEEL VNFIERVPKA QSASLWKNKD IQRLLPSPLK DAVEVSTWRR
CYTMQEVNSA LSKIQLVGYS QKIELFGAVQ VTPLSSGYAL GSSNWIIQSH YEKVSYVSGS
SLLTTHPQPM DQASLKNSDV LILTGLTQIP TANPDGMVGE FCSNLALTVR NGGNVLVPCY
PSGVIYDLLE CLYQYIDSAG LSNIPFYFIS PVANSSLEFS QIFAEWLCHN KQSKVYLPEP
PFPHAELIQT NKLKHYRSIH GDFSNDFRQP CVLFTGHPSL RFGDVVHFME LWGKSSLNTI
IFTEPDFSYL EALAPYQPLA MKCIYCPIDT RLNFIQVSKL LKEVQPLHVV CPEQYTQPPP
AQAHRMDLMI DCQPPAMSYR RAEVLALPFK RRYEKIEIMP ELADSLVPME IKPGISLATV
SAVLHTKDNK HVLQPPPKPT QPTSSKKRKR VNEDIPDCKV LKPLLSGSIP VEQFVQTLEK
HGFSDIKVED TAKGHIVLLQ EAETLIQIEE DSTHIICDND ETLRVRLRDL VLRFLQKF
