INTA_ECOLI
ID INTA_ECOLI Reviewed; 413 AA.
AC P32053;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Prophage integrase IntA {ECO:0000305};
DE AltName: Full=Prophage CP4-57 integrase SlpA {ECO:0000303|PubMed:7511583};
GN Name=intA; Synonyms=intX, slpA {ECO:0000303|PubMed:7511583};
GN OrderedLocusNames=b2622, JW2602;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY ALPA, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12;
RX PubMed=7511583; DOI=10.1128/jb.176.7.2068-2081.1994;
RA Kirby J.E., Trempy J.E., Gottesman S.;
RT "Excision of a P4-like cryptic prophage leads to Alp protease expression in
RT Escherichia coli.";
RL J. Bacteriol. 176:2068-2081(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-99.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.
RC STRAIN=K12;
RX PubMed=7524073; DOI=10.1073/pnas.91.20.9223;
RA Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.;
RT "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from
RT Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994).
RN [6]
RP FUNCTION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=7511582; DOI=10.1128/jb.176.7.2061-2067.1994;
RA Trempy J.E., Kirby J.E., Gottesman S.;
RT "Alp suppression of Lon: dependence on the slpA gene.";
RL J. Bacteriol. 176:2061-2067(1994).
CC -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC host genome by site-specific recombination. In conjunction with
CC excisionase, integrase is also necessary for excision of the prophage
CC from the host genome. Part of the cryptic P4-like prophage CP4-57, it
CC excises the prophage when overexpressed, which also requires
CC integration host factor (encoded by ihfA and ihfB) (PubMed:7511583).
CC Overexpression of AlpA leads to excision of the CP4-57 prophage, which
CC inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA
CC which is required to rescue stalled ribosomes in a process known as
CC trans-translation (PubMed:7511582). {ECO:0000269|PubMed:7511583}.
CC -!- INTERACTION:
CC P32053; P06959: aceF; NbExp=3; IntAct=EBI-552967, EBI-542707;
CC P32053; P06864: ebgA; NbExp=3; IntAct=EBI-552967, EBI-558098;
CC -!- INDUCTION: By AlpA (PubMed:7511582). {ECO:0000269|PubMed:7511582}.
CC -!- DISRUPTION PHENOTYPE: Loss of excision of its prophage
CC (PubMed:7511583). {ECO:0000269|PubMed:7511583}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U03737; AAA18417.1; -; Unassigned_DNA.
DR EMBL; U36840; AAA79791.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75670.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA20921.2; -; Genomic_DNA.
DR EMBL; D12501; BAA20981.1; -; Genomic_DNA.
DR PIR; H65040; H65040.
DR RefSeq; NP_417111.1; NC_000913.3.
DR RefSeq; WP_000101723.1; NZ_LN832404.1.
DR AlphaFoldDB; P32053; -.
DR SMR; P32053; -.
DR BioGRID; 4260622; 13.
DR BioGRID; 850520; 1.
DR DIP; DIP-10034N; -.
DR IntAct; P32053; 26.
DR STRING; 511145.b2622; -.
DR PaxDb; P32053; -.
DR PRIDE; P32053; -.
DR EnsemblBacteria; AAC75670; AAC75670; b2622.
DR EnsemblBacteria; BAA20921; BAA20921; BAA20921.
DR GeneID; 946160; -.
DR KEGG; ecj:JW2602; -.
DR KEGG; eco:b2622; -.
DR PATRIC; fig|1411691.4.peg.4117; -.
DR EchoBASE; EB1731; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_027562_0_0_6; -.
DR InParanoid; P32053; -.
DR OMA; DRNPRTH; -.
DR PhylomeDB; P32053; -.
DR BioCyc; EcoCyc:EG11783-MON; -.
DR PRO; PR:P32053; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008979; F:prophage integrase activity; IMP:EcoCyc.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032359; P:provirus excision; IMP:EcoCyc.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR Gene3D; 3.30.160.390; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR038488; Integrase_DNA-bd_sf.
DR InterPro; IPR025166; Integrase_DNA_bind_dom.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR Pfam; PF13356; Arm-DNA-bind_3; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW Viral genome integration; Virus entry into host cell.
FT CHAIN 1..413
FT /note="Prophage integrase IntA"
FT /id="PRO_0000197511"
FT DOMAIN 105..186
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 209..386
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 248
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 275
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 337
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 340
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 363
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 373
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 413 AA; 46652 MW; E050A1F60860BCB5 CRC64;
MARKTKPLTD TEIKAAKPKD ADYQLYDGDG LTLLIKSSGS KLWQFRYYRP LTKQRTKQSF
GAYPAVSLSD ARKLRAESKV LLAKDIDPQE HQKEQVRNSQ EAKTNTFLLV AERWWNVKKT
SVTEDYADDI WRSLERDIFP AIGDISITEI KAHTLVKAVQ PVQARGALET VRRLCQRINE
VMIYAQNTGL IDAVPSVNIG KAFEKPQKKN MPSIRPDQLP QLMHTMRTAS ISMSTRCLFM
WQLLTITRPA EAAEARWDEI DFNASEWKIP AARMKMNRDH TVPLSDGALA ILEMMKPLSG
GREFIFPSRI KPNQPMNSQT VNAALKRAGL GGVLVSHGLR SIASTALNEE GFPPDVIEAA
LAHVDKNEVR RAYNRSDYLE QRRPMMQWWA DLVKAADSGS IVLTHLSKIR LVG
