INTB_ECOLI
ID INTB_ECOLI Reviewed; 396 AA.
AC P39347; Q2M639;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Putative protein IntB {ECO:0000305};
DE AltName: Full=Int(P4);
DE AltName: Full=Putative prophage P4 integrase;
GN Name=intB; OrderedLocusNames=b4271, JW4227;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-115.
RC STRAIN=B;
RA Pucci M.J., Discotto L.F., Dougherty T.J.;
RL Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC -!- CAUTION: Additional homology to the integrase family exists upstream of
CC the current start codon. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U14003; AAA97167.1; -; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; BAE78267.1; -; Genomic_DNA.
DR EMBL; M96355; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S56496; S56496.
DR AlphaFoldDB; P39347; -.
DR BioGRID; 4262732; 18.
DR DIP; DIP-10035N; -.
DR IntAct; P39347; 15.
DR STRING; 316407.85677017; -.
DR PRIDE; P39347; -.
DR EnsemblBacteria; BAE78267; BAE78267; BAE78267.
DR KEGG; ecj:JW4227; -.
DR PATRIC; fig|83333.103.peg.579; -.
DR EchoBASE; EB2267; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_027562_0_1_6; -.
DR InParanoid; P39347; -.
DR OMA; AVARDWH; -.
DR PhylomeDB; P39347; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR Gene3D; 3.30.160.390; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR038488; Integrase_DNA-bd_sf.
DR InterPro; IPR025166; Integrase_DNA_bind_dom.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR Pfam; PF13356; Arm-DNA-bind_3; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 5: Uncertain;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW Viral genome integration; Virus entry into host cell.
FT CHAIN 1..396
FT /note="Putative protein IntB"
FT /id="PRO_0000197512"
FT DOMAIN 71..151
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 174..367
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 252
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 316
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 319
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 343
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 353
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 396 AA; 45582 MW; DE3762C1EFC8BFCC CRC64;
MHLLVHPNGS KYWRLQYRYE GKQKMLALGV YPEITLADAR VRRDEARKLL ANGVDPGDKK
KNDKVEQSKA RTFKEVAIEW HGTNKKWSED HAHRVLKSLE DNLFAALGER NIAELKTRDL
LAPIKAVEMS GRLEVAARLQ QRTTAIMRYA VQSGLIDYNP AQEMAGAVAS CNRQHRPALE
LKRIPELLTK IDSYTGRPLT RWAIELTLLI FIRSSELRFA RWSEIDFEAS IWTIPPEREP
IPGVKHSHRG SKMRTTHLVP LSTQALAILK QIKQFYGAHD LIFIGDHDSH KPMSENTVNS
ALRVMGYDTK VEVCGHGFRT MACSSLVESG LWSRDAVERQ MSHMARNSVR AAYIHKAEHL
EERRLMLQWW ADFLDVNRER FISPFEYAKI NNPLKQ
