ID   INTD_ECOLI              Reviewed;         387 AA.
AC   P24218; Q2MBP3;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Prophage integrase IntD {ECO:0000305};
DE   AltName: Full=Prophage DLP12 integrase;
DE   AltName: Full=Prophage QSR' integrase;
GN   Name=intD; Synonyms=int; OrderedLocusNames=b0537, JW0525;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2553674; DOI=10.1128/jb.171.11.6197-6205.1989;
RA   Lindsey D.F., Mullin D.A., Walker J.R.;
RT   "Characterization of the cryptic lambdoid prophage DLP12 of Escherichia
RT   coli and overlap of the DLP12 integrase gene with the tRNA gene argU.";
RL   J. Bacteriol. 171:6197-6205(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2183007; DOI=10.1007/bf00260503;
RA   Muramatsu S., Mizuno T.;
RT   "Nucleotide sequence of the region encompassing the int gene of a cryptic
RT   prophage and the DNA Y gene flanked by a curved DNA sequence of Escherichia
RT   coli K12.";
RL   Mol. Gen. Genet. 220:325-328(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Integrase from the cryptic lambdoid prophage DLP12. Integrase
CC       is necessary for integration of the phage into the host genome by site-
CC       specific recombination. In conjunction with excisionase, integrase is
CC       also necessary for excision of the prophage from the host genome.
CC   -!- MISCELLANEOUS: Encoded by the cryptic lambdoid prophage DLP12.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M31074; AAA65483.1; -; Genomic_DNA.
DR   EMBL; M27155; AAA24030.1; -; Genomic_DNA.
DR   EMBL; X51662; CAA35974.1; -; Genomic_DNA.
DR   EMBL; U82598; AAB40734.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73638.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76313.1; -; Genomic_DNA.
DR   PIR; A33497; A33497.
DR   RefSeq; NP_415069.1; NC_000913.3.
DR   RefSeq; WP_001350488.1; NZ_LN832404.1.
DR   AlphaFoldDB; P24218; -.
DR   SMR; P24218; -.
DR   BioGRID; 4259881; 12.
DR   BioGRID; 851494; 1.
DR   IntAct; P24218; 20.
DR   STRING; 511145.b0537; -.
DR   jPOST; P24218; -.
DR   PaxDb; P24218; -.
DR   PRIDE; P24218; -.
DR   EnsemblBacteria; AAC73638; AAC73638; b0537.
DR   EnsemblBacteria; BAE76313; BAE76313; BAE76313.
DR   GeneID; 947162; -.
DR   KEGG; ecj:JW0525; -.
DR   KEGG; eco:b0537; -.
DR   PATRIC; fig|1411691.4.peg.1741; -.
DR   EchoBASE; EB0502; -.
DR   eggNOG; COG0582; Bacteria.
DR   HOGENOM; CLU_027562_17_7_6; -.
DR   InParanoid; P24218; -.
DR   OMA; AWIHADQ; -.
DR   PhylomeDB; P24218; -.
DR   BioCyc; EcoCyc:EG10507-MON; -.
DR   PRO; PR:P24218; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008979; F:prophage integrase activity; ISS:EcoCyc.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..387
FT                   /note="Prophage integrase IntD"
FT                   /id="PRO_0000197513"
FT   DOMAIN          57..161
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          181..362
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        215
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        242
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        314
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        317
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        340
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        349
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   387 AA;  45090 MW;  E3DB8A8D0A613F64 CRC64;
     MSLFRRNEIW YASYSLPGGK RIKESLGTKD KRQAQELHDK RKAELWRVEK LGDLPDVTFE
     EACLRWLEEK ADKKSLDSDK SRIEFWLEHF EGIRLKDISE AKIYSAVSRM HNRKTKEIWK
     QKVQAAIRKG KELPVYEPKP VSTQTKAKHL AMIKAILRAA ERDWKWLEKA PVIKIPAVRN
     KRVRWLEKEE AKRLIDECPE PLKSVVKFAL ATGLRKSNII NLEWQQIDMQ RRVAWVNPEE
     SKSNRAIGVA LNDTACKVLR DQIGKHHKWV FVHTKAAKRA DGTSTPAVRK MRIDSKTSWL
     SACRRAGIED FRFHDLRHTW ASWLIQSGVP LSVLQEMGGW ESIEMVRRYA HLAPNHLTEH
     ARKIDDIFGD NVPNMSHSEI MEDIKKA