INTE_ECOLI
ID INTE_ECOLI Reviewed; 375 AA.
AC P75969;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Prophage integrase IntE {ECO:0000305};
DE AltName: Full=Int(Lambda);
DE AltName: Full=Prophage e14 integrase;
DE AltName: Full=Prophage lambda integrase;
GN Name=intE; Synonyms=ycfI; OrderedLocusNames=b1140, JW1126;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Integrase from the cryptic lambdoid prophage e14. Integrase
CC is necessary for integration of the phage into the host genome by site-
CC specific recombination. In conjunction with excisionase, integrase is
CC also necessary for excision of the prophage from the host genome.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; U00096; AAC74224.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35969.1; -; Genomic_DNA.
DR PIR; A64859; A64859.
DR RefSeq; NP_415658.1; NC_000913.3.
DR RefSeq; WP_000741310.1; NZ_CP010440.1.
DR AlphaFoldDB; P75969; -.
DR SMR; P75969; -.
DR BioGRID; 4263213; 16.
DR DIP; DIP-10037N; -.
DR IntAct; P75969; 3.
DR STRING; 511145.b1140; -.
DR PaxDb; P75969; -.
DR PRIDE; P75969; -.
DR EnsemblBacteria; AAC74224; AAC74224; b1140.
DR EnsemblBacteria; BAA35969; BAA35969; BAA35969.
DR GeneID; 948034; -.
DR KEGG; ecj:JW1126; -.
DR KEGG; eco:b1140; -.
DR PATRIC; fig|511145.12.peg.1186; -.
DR EchoBASE; EB2273; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_049005_0_1_6; -.
DR PhylomeDB; P75969; -.
DR BioCyc; EcoCyc:EG12370-MON; -.
DR PRO; PR:P75969; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008907; F:integrase activity; IEA:InterPro.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:UniProtKB.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR015094; Integrase_lambda-typ_DNA-bd_N.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR Pfam; PF09003; Arm-DNA-bind_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW Viral genome integration; Virus entry into host cell.
FT CHAIN 1..375
FT /note="Prophage integrase IntE"
FT /id="PRO_0000197514"
FT DOMAIN 82..167
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 189..375
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT REGION 350..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..375
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 226
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 249
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 330
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 333
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 353
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 362
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 375 AA; 42799 MW; 8F49F9805C7AA2B0 CRC64;
MAARPRKNNV SVPNLYPLYS RKVNKVYWRY KHPVTGKFHA LGTNEAEAIA IATEANTRLA
EQRTRQILAI SDRIATSKGK AITTSTWLDR YQAIQDDRLK SGDIRLNTYK QKAKPVSLLR
ERAGMKLISA VDVRDIAQLL DEYIAAGRPR MAQVVRSVLI DVFKEAQHYG EVPPGYNPAL
ATKQPRRKIT RQRLSLEEWK KIFDIADATH RYMGNAMLLA LVTGQRLGDI SRMKFSDIWD
DHLHVIQEKT GSKIAIPLSL RLNAINWSLR DVVARCRDYA VSAYLVHFFR STSQAERGAQ
VKANTLTMNF SKARDLARID WGEGSPATFH EQRSLSERLY KEQGLDTQKL LGHKTQQQTD
RYHDDRGKGW SKVAL
