INTF_ECOLI
ID INTF_ECOLI Reviewed; 466 AA.
AC P71298; Q2MCE1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Prophage integrase IntF {ECO:0000305};
DE AltName: Full=Putative prophage CP4-6 integrase;
GN Name=intF; Synonyms=yagO; OrderedLocusNames=b0281, JW0275;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC host genome by site-specific recombination. In conjunction with
CC excisionase, integrase is also necessary for excision of the prophage
CC from the host genome.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; U73857; AAB18010.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73384.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76065.1; -; Genomic_DNA.
DR PIR; A64754; A64754.
DR RefSeq; NP_414815.1; NC_000913.3.
DR RefSeq; WP_001407714.1; NZ_LN832404.1.
DR AlphaFoldDB; P71298; -.
DR SMR; P71298; -.
DR BioGRID; 4259784; 13.
DR IntAct; P71298; 2.
DR STRING; 511145.b0281; -.
DR jPOST; P71298; -.
DR PaxDb; P71298; -.
DR PRIDE; P71298; -.
DR EnsemblBacteria; AAC73384; AAC73384; b0281.
DR EnsemblBacteria; BAE76065; BAE76065; BAE76065.
DR GeneID; 947351; -.
DR KEGG; ecj:JW0275; -.
DR KEGG; eco:b0281; -.
DR PATRIC; fig|511145.12.peg.285; -.
DR EchoBASE; EB3324; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_027562_3_0_6; -.
DR InParanoid; P71298; -.
DR OMA; WRSLTMN; -.
DR PhylomeDB; P71298; -.
DR BioCyc; EcoCyc:G6152-MON; -.
DR PRO; PR:P71298; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0010165; P:response to X-ray; IMP:EcoCyc.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.443.10; -; 1.
DR Gene3D; 3.30.160.390; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR038488; Integrase_DNA-bd_sf.
DR InterPro; IPR025166; Integrase_DNA_bind_dom.
DR Pfam; PF13356; Arm-DNA-bind_3; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW Viral genome integration; Virus entry into host cell.
FT CHAIN 1..466
FT /note="Prophage integrase IntF"
FT /id="PRO_0000197515"
FT DOMAIN 134..239
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 268..445
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 306
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 328
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 396
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 399
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 422
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 432
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 466 AA; 53074 MW; 4876E3F1898CE465 CRC64;
MFIPSIYLHQ QLHYCKTAIL NWSRKMALSR QKFTFERLRR FTLPEGKKQT FLWDADVTTL
ACRATSGAKA FVFQSVYAGK TLRMTIGNIN DWKIDDARAE ARRLQTLIDT GIDPRIAKAV
KIAEAESLQA ESRKTKVTFS VAWEDYLQEL RTGISAKTKR PYSTRYIADH INLSSRGGES
KKRGQGPTSA GPLASLLNLP LSELTPDYIA AWLSTERQNR PTVTAHAYRL LRAFIKWSNY
QKKYQGIIPG DLAQDYNVRK MVPVSASKAD DCLQKEQLKS WFSAVRSLNN PIASAYLQVL
LLTGARREEI ASLRWSDVDF KWSSMRIKDK IEGERIIPLT PYVSELLNVL AQSPNSDVNK
EGWVFRSNSK SGKIIEPRSA HNRALVLAEL PHISLHGLRR SFGTLAEWVE VPTGIVAQIM
GHKPSALAEK HYRRRPLDLL RKWHEKIETW ILNEAGITIK NNVDMR
