ID   INTL_ECOLX              Reviewed;         246 AA.
AC   Q47036;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Probable site-specific recombinase in afa region;
GN   Name=int;
OS   Escherichia coli.
OG   Plasmid pIL1055.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A30 / UPEC;
RX   PubMed=8002584; DOI=10.1128/jb.176.24.7601-7613.1994;
RA   Garcia M.-I., Labigne A., le Bouguenec C.L.;
RT   "Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster
RT   and its translocation via flanking IS1 insertion sequences.";
RL   J. Bacteriol. 176:7601-7613(1994).
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X76688; CAA54111.1; -; Genomic_DNA.
DR   PIR; B55545; B55545.
DR   AlphaFoldDB; Q47036; -.
DR   SMR; Q47036; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR016423; Resolvase_Rsv.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   PIRSF; PIRSF004576; Resolvase_Rsv; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..246
FT                   /note="Probable site-specific recombinase in afa region"
FT                   /id="PRO_0000197545"
FT   DOMAIN          40..225
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        102
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        177
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        203
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        212
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   246 AA;  28108 MW;  67772F861756AA29 CRC64;
     MNNVIPLQNS PERVSLLPIA PGVDFATALS LRRMATSTGA TPAYLLAPEV SALLFYMPDQ
     RHHMLFATLW NTGMRIGEAR IVTPESFDLD GVRPFVRVLS EKVRARRGRP PKDEVRLVPL
     TDISYVRQME SWMITTRPRR REPLWAVTDE TMRNWLKQAV RRAEADGVHF SISVTPHTFR
     HSYIMHMLYH RQPRKVIQAL AGHRDPRSME VYTRVFALDM AATLAVPFTG DGRDAAEILR
     TLPPLK