ID   INTM_ECOLI              Reviewed;         246 AA.
AC   P56979;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Probable site-specific recombinase;
GN   Name=intM; Synonyms=int; OrderedLocusNames=ECOK12F032;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; AP001918; BAA97902.1; -; Genomic_DNA.
DR   RefSeq; NP_061411.1; NC_002483.1.
DR   RefSeq; WP_001066941.1; NZ_CP014273.1.
DR   AlphaFoldDB; P56979; -.
DR   SMR; P56979; -.
DR   PRIDE; P56979; -.
DR   PhylomeDB; P56979; -.
DR   PRO; PR:P56979; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR016423; Resolvase_Rsv.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   PIRSF; PIRSF004576; Resolvase_Rsv; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..246
FT                   /note="Probable site-specific recombinase"
FT                   /id="PRO_0000197544"
FT   DOMAIN          40..225
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        102
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        177
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        203
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        212
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   246 AA;  28164 MW;  8B72B4814C415DB0 CRC64;
     MNNVIPLQNS PERVSLLPIA PGVDFATALS LRRMATSTGA TPAYLLAPEV SALLFYMPDQ
     RHHMLFATLW NTGMRIGEAR MLTPESFDLD GVRPFVRILS EKVRARRGRP PKDEVRLVPL
     TDISYVRQME SWMITTRPRR REPLWAVTDE TMRNWLKQAV RRAEADGVHF SIPVTPHTFR
     HSYIMHMLYH RQPRKVIQAL AGHRDPRSME VYTRVFALDM AATLAVPFTG DGRDAAEILR
     TLPPLK