ID   INTQ_ECOLI              Reviewed;         398 AA.
AC   P76168; Q2MB81;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 3.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=Putative defective protein IntQ {ECO:0000305};
DE   AltName: Full=Putative lambdoid prophage Qin defective integrase;
GN   Name=intQ; OrderedLocusNames=b1579, JW1571;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       P76168; P04994: xseA; NbExp=3; IntAct=EBI-559711, EBI-559703;
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC   -!- CAUTION: May not be functional, the original sequence is interrupted by
CC       an IS2 element. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAE76475.1; -; Genomic_DNA.
DR   PIR; E64913; E64913.
DR   AlphaFoldDB; P76168; -.
DR   SMR; P76168; -.
DR   BioGRID; 4260817; 24.
DR   DIP; DIP-10039N; -.
DR   IntAct; P76168; 6.
DR   STRING; 316407.85675030; -.
DR   PRIDE; P76168; -.
DR   EnsemblBacteria; BAE76475; BAE76475; BAE76475.
DR   KEGG; ecj:JW1571; -.
DR   eggNOG; COG0582; Bacteria.
DR   HOGENOM; CLU_027562_8_1_6; -.
DR   InParanoid; P76168; -.
DR   PRO; PR:P76168; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR022000; Min27-like_integrase_DNA_bind.
DR   Pfam; PF12167; Arm-DNA-bind_2; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   1: Evidence at protein level;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..398
FT                   /note="Putative defective protein IntQ"
FT                   /id="PRO_0000197516"
FT   DOMAIN          51..146
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          167..378
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        202
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        331
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        354
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        364
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   CONFLICT        274
FT                   /note="F -> L (in Ref. 2; BAE76475)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   398 AA;  45262 MW;  D9A24416922D80CF CRC64;
     MITDVWKYRG KSTGELRSSV CYAIKTGVFD YAKQFPSSRN LEKFGEARQD LTIKELAEKF
     LALKETEVAK TSLNTYRAVI KNILSIIGEK NLASSINKEK LLEVRKELLT GYQIPKSNYI
     VTQPGRSAVT VNNYMTNLNA VFQFGVDNGY LADNPFKGIS PLKESRTIPD PLSREEFIRL
     IDACRNQQAK NLWCVSVYTG VRPGELCALG WEDIDLKNGT MMIRRNLAKD RFTVPKTQAG
     TNRVIHLIKP AIDALRSQMT LTRLSKEHII DVHFREYGRT EKQKCTFVFQ PEVSARVKNY
     GDHFTVDSIR QMWDAAIKRA GLRHRKSYQS RHTYACWSLT AGANPAFIAN QMGHADAQMV
     FQVYGKWMSE NNNAQVALLN TQLSEFAPTM PHNEAMKN