ID   INTR_ECOLI              Reviewed;         411 AA.
AC   P76056; Q2MBE8;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Prophage integrase IntR {ECO:0000305};
DE   AltName: Full=Putative lambdoid prophage Rac integrase;
GN   Name=intR; Synonyms=ydaP; OrderedLocusNames=b1345, JW1339;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; U00096; AAC74427.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76408.1; -; Genomic_DNA.
DR   PIR; D64884; D64884.
DR   RefSeq; NP_415861.1; NC_000913.3.
DR   RefSeq; WP_000040852.1; NZ_CP047127.1.
DR   AlphaFoldDB; P76056; -.
DR   SMR; P76056; -.
DR   BioGRID; 4261947; 7.
DR   IntAct; P76056; 6.
DR   STRING; 511145.b1345; -.
DR   PaxDb; P76056; -.
DR   PRIDE; P76056; -.
DR   EnsemblBacteria; AAC74427; AAC74427; b1345.
DR   EnsemblBacteria; BAE76408; BAE76408; BAE76408.
DR   GeneID; 66674827; -.
DR   GeneID; 946976; -.
DR   KEGG; ecj:JW1339; -.
DR   KEGG; eco:b1345; -.
DR   PATRIC; fig|1411691.4.peg.931; -.
DR   EchoBASE; EB3141; -.
DR   eggNOG; COG0582; Bacteria.
DR   HOGENOM; CLU_027562_8_1_6; -.
DR   InParanoid; P76056; -.
DR   OMA; GIRCREV; -.
DR   PhylomeDB; P76056; -.
DR   BioCyc; EcoCyc:G6676-MON; -.
DR   PRO; PR:P76056; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR022000; Min27-like_integrase_DNA_bind.
DR   Pfam; PF12167; Arm-DNA-bind_2; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..411
FT                   /note="Prophage integrase IntR"
FT                   /id="PRO_0000197517"
FT   DOMAIN          81..176
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          197..404
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        231
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        266
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        358
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        381
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        391
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   411 AA;  47539 MW;  E14B91630E0EF9A5 CRC64;
     MSKLPTGVEI RGRYIRIWFM FRGKRCRETL KGWEITNSNI KKAGNLRALI VHEINSGEFE
     YLRRFPQSST GAKMVTTRVI KTFGELCDIW TKIKETELTT NTMKKTKSQL KTLRIIICES
     TPISHIRYSD ILNYRNELLH GETLYLDNPR SNKKGRTVRT VDNYIALLCS LLRFAYQSGF
     ISTKPFEGVK KLQRNRIKPD PLSKTEFNAL MESEKGQSQN LWKFAVYSGL RHGELAALAW
     EDVDLEKGIV NVRRNLTILD MFGPPKTNAG IRTVTLLQPA LEALKEQYKL TGHHRKSEIT
     FYHREYGRTE KQKLHFVFMP RVCNGKQKPY YSVSSLGARW NAAVKRAGIR RRNPYHTRHT
     FACWLLTAGA NPAFIASQMG HETAQMVYEI YGMWIDDMND EQIAMLNARL S