INTR_SACER
ID INTR_SACER Reviewed; 437 AA.
AC P22877;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Integrase;
DE AltName: Full=Recombinase;
GN Name=int;
OS Saccharopolyspora erythraea (Streptomyces erythraeus).
OG Plasmid pSE211.
OC Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC Saccharopolyspora.
OX NCBI_TaxID=1836;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ER720;
RX PubMed=2180909; DOI=10.1128/jb.172.4.1877-1888.1990;
RA Brown D.P., Idler K.B., Katz L.;
RT "Characterization of the genetic elements required for site-specific
RT integration of plasmid pSE211 in Saccharopolyspora erythraea.";
RL J. Bacteriol. 172:1877-1888(1990).
CC -!- FUNCTION: Is a recombinase (or integrase), catalyzing the cutting and
CC rejoining of the recombining DNA molecules.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; M35138; AAA98345.1; -; Genomic_DNA.
DR PIR; C35147; C35147.
DR RefSeq; WP_009946133.1; NZ_CP069353.1.
DR AlphaFoldDB; P22877; -.
DR SMR; P22877; -.
DR OMA; RHVERKT; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR Pfam; PF14659; Phage_int_SAM_3; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Plasmid;
KW Viral genome integration; Virus entry into host cell.
FT CHAIN 1..437
FT /note="Integrase"
FT /id="PRO_0000197534"
FT DOMAIN 73..158
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 179..428
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 214
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 245
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 379
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 382
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 405
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 414
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 437 AA; 50324 MW; 29EED7108CC5AFBD CRC64;
MEQKRTRNPN GRSTIYLGND GYWHGRVTMG IGDDGKPDRR HVKRKDKDEV VEEVGKLERE
RDSGNVRKKG QPWTVERWLT HWVESIAPLT CRYKTMRGYQ TAVYKHLIPG LGAHRLDRIQ
NHPEYFEKFY LRMIESGLKP ATAHQVHRTA RTAFGEAYKR GRIQRNPVSI AKAPRVEEEE
VEPLEVEDMQ LVIKAALERR NGVRYVIALA LGTRQGESLA LKWPRLNRQK RTLRITKALQ
RQTWKHGCSD PHRCGATYHK TEPCKAACKR HTRACPPPCP PACTEHARWC PQRTGGGLVE
VDVKSRAGRR TVTLPDQLFD LILKHEKLQG AERELAGTEW HDGEWMFTQP NGKPIDPRQD
LDEWKAILVE AGVREARLHD ARHTAATVLL VLGVPDRVVM ELMGWSSVTM KQRYMHVIDS
VRNDVADRLN TYFWGTN
