ID   INTR_STRAM              Reviewed;         388 AA.
AC   P15435;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Integrase;
GN   Name=int;
OS   Streptomyces ambofaciens.
OG   Plasmid pSAM2.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1889;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX   PubMed=2721504; DOI=10.1002/j.1460-2075.1989.tb03460.x;
RA   Boccard F., Smokvina T., Pernodet J.L., Friedmann A., Guerineau M.;
RT   "The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is
RT   related to temperate bacteriophages.";
RL   EMBO J. 8:973-980(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15154 / NBRC 13685 / NRRL 2420 / Isolate B3;
RX   PubMed=9620953; DOI=10.1128/jb.180.12.3056-3061.1998;
RA   Sezonov G., Duchene A.M., Friedmann A., Guerineau M., Pernodet J.L.;
RT   "Replicase, excisionase, and integrase genes of the Streptomyces element
RT   pSAM2 constitute an operon positively regulated by the pra gene.";
RL   J. Bacteriol. 180:3056-3061(1998).
CC   -!- FUNCTION: Required for integration of pSAM2.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X14899; CAA33029.1; -; Genomic_DNA.
DR   EMBL; AJ005260; CAA06457.1; -; Genomic_DNA.
DR   PIR; S04110; S04110.
DR   AlphaFoldDB; P15435; -.
DR   SMR; P15435; -.
DR   OMA; MARDKVK; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..388
FT                   /note="Integrase"
FT                   /id="PRO_0000197533"
FT   DOMAIN          70..151
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          173..379
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        208
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        249
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        330
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        353
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        363
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   388 AA;  43059 MW;  72FD7562617C974C CRC64;
     MAKRRSRGDG GLHWDEKRQR WIATANLGFD PSGKRIVKRG SGKTKTEAKN KLKEVLRDHE
     DGLAIAPTGY TVADAVNDWL AYGLAGRDQR TVENCTHLSQ KHVIPGLGAR KLRDLSAEDV
     DRWLAAKAQT LSTRSLQAVH SCLNRAVKRA MARDKVKRNV VELCSVPQGQ PGRPSKALTF
     AQAEAVLNAA EGTSMHAYIV VALLTGARTE ELRALTWDHV FLKGSPDVEP PQPPHIAVWR
     SVRRGGDTKT RKSRRTLALP ARCVEVLWQH FEDQGWERLA AGDKWEEHGL VFSSAVGKPL
     DATNVRRAFR QALKDANGIN ADEWTPRELR HSFVSLLSDR GVPLEEISRL VGHSGTAVTE
     EVYRKQIRPV IQTGAVVMDG IFKRGPAR