ID   INTS_ECOLI              Reviewed;         385 AA.
AC   P37326; P76942;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=Prophage integrase IntS {ECO:0000305};
DE   AltName: Full=Putative prophage CPS-53 integrase;
GN   Name=intS; Synonyms=intC, yfdB; OrderedLocusNames=b2349, JW2345;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RA   Baumann S.;
RL   Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5] {ECO:0007744|PDB:2KJ8}
RP   STRUCTURE BY NMR OF 87-196.
RA   Cort J.R., Montelione G.T., Kennedy M.A.;
RT   "NMR structure of fragment 87-196 from the putative phage integrase IntS of
RT   E. coli.";
RL   Submitted (MAY-2009) to the PDB data bank.
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; U11296; AAA19609.1; -; Unassigned_DNA.
DR   EMBL; U00096; AAC75408.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16208.1; -; Genomic_DNA.
DR   PIR; I84552; I84552.
DR   RefSeq; NP_416850.1; NC_000913.3.
DR   RefSeq; WP_000958671.1; NZ_LN832404.1.
DR   PDB; 2KJ8; NMR; -; A=87-196.
DR   PDBsum; 2KJ8; -.
DR   AlphaFoldDB; P37326; -.
DR   SMR; P37326; -.
DR   BioGRID; 4259384; 12.
DR   DIP; DIP-10041N; -.
DR   IntAct; P37326; 12.
DR   STRING; 511145.b2349; -.
DR   PaxDb; P37326; -.
DR   PRIDE; P37326; -.
DR   EnsemblBacteria; AAC75408; AAC75408; b2349.
DR   EnsemblBacteria; BAA16208; BAA16208; BAA16208.
DR   GeneID; 946821; -.
DR   KEGG; ecj:JW2345; -.
DR   KEGG; eco:b2349; -.
DR   PATRIC; fig|511145.12.peg.2445; -.
DR   EchoBASE; EB2312; -.
DR   eggNOG; COG0582; Bacteria.
DR   HOGENOM; CLU_027562_0_0_6; -.
DR   InParanoid; P37326; -.
DR   OMA; YIGHRPI; -.
DR   PhylomeDB; P37326; -.
DR   BioCyc; EcoCyc:G7218-MON; -.
DR   EvolutionaryTrace; P37326; -.
DR   PRO; PR:P37326; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0032359; P:provirus excision; IMP:EcoCyc.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   Gene3D; 3.30.160.390; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR038488; Integrase_DNA-bd_sf.
DR   InterPro; IPR025166; Integrase_DNA_bind_dom.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF13356; Arm-DNA-bind_3; 1.
DR   Pfam; PF14659; Phage_int_SAM_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA integration; DNA recombination; DNA-binding;
KW   Reference proteome; Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..385
FT                   /note="Prophage integrase IntS"
FT                   /id="PRO_0000197518"
FT   DOMAIN          91..172
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          195..373
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        261
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        324
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        327
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        350
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        360
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   HELIX           93..104
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   TURN            105..107
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   HELIX           110..128
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   HELIX           138..149
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   TURN            150..152
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   HELIX           154..173
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   HELIX           182..191
FT                   /evidence="ECO:0007829|PDB:2KJ8"
FT   HELIX           192..194
FT                   /evidence="ECO:0007829|PDB:2KJ8"
SQ   SEQUENCE   385 AA;  44061 MW;  C3D9B1C59A06DBBA CRC64;
     MLTVKQIEAA KPKEKPYRLL DGNGLYLYVP VSGKKVWQLR YKIDGKEKIL TVGKYPLMTL
     QEARDKAWTA RKDISVGIDP VKAKKASSNN NSFSAIYKEW YEHKKQVWSV GYATELAKMF
     DDDILPIIGG LEIQDIEPMQ LLEVIRRFED RGAMERANKA RRRCGEVFRY AIVTGRAKYN
     PAPDLADAMK GYRKKNFPFL PADQIPAFNK ALATFSGSIV SLIATKVLRY TALRTKELRS
     MLWKNVDFEN RIITIDASVM KGRKIHVVPM SDQVVELLTT LSSITKPVSE FVFAGRNDKK
     KPICENAVLL VIKQIGYEGL ESGHGFRHEF STIMNEHEWP ADAIEVQLAH ANGGSVRGIY
     NHAQYLDKRR EMMQWWADWL DEKVE