INTU_DROME
ID INTU_DROME Reviewed; 869 AA.
AC Q9VPH0; Q24144;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein inturned;
GN Name=in; ORFNames=CG16993;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=8631273; DOI=10.1242/dev.122.3.961;
RA Park W.J., Liu J., Sharp E.J., Adler P.N.;
RT "The Drosophila tissue polarity gene inturned acts cell autonomously and
RT encodes a novel protein.";
RL Development 122:961-969(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=10570461;
RX DOI=10.1002/(sici)1520-6408(1999)25:4<297::aid-dvg3>3.0.co;2-l;
RA Yun U.J., Kim S.Y., Liu J., Adler P.N., Bae E., Kim J., Park W.J.;
RT "The inturned protein of Drosophila melanogaster is a cytoplasmic protein
RT located at the cell periphery in wing cells.";
RL Dev. Genet. 25:297-305(1999).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=15556868; DOI=10.1016/j.cub.2004.11.007;
RA Adler P.N., Zhu C., Stone D.;
RT "Inturned localizes to the proximal side of wing cells under the
RT instruction of upstream planar polarity proteins.";
RL Curr. Biol. 14:2046-2051(2004).
RN [7]
RP FUNCTION.
RX PubMed=11973308; DOI=10.1093/genetics/160.4.1535;
RA Lee H., Adler P.N.;
RT "The function of the frizzled pathway in the Drosophila wing is dependent
RT on inturned and fuzzy.";
RL Genetics 160:1535-1547(2002).
RN [8]
RP FUNCTION.
RX PubMed=18701542; DOI=10.1242/dev.025205;
RA Strutt D., Warrington S.J.;
RT "Planar polarity genes in the Drosophila wing regulate the localisation of
RT the FH3-domain protein Multiple Wing Hairs to control the site of hair
RT production.";
RL Development 135:3103-3111(2008).
RN [9]
RP FUNCTION, AND INTERACTION WITH MWH.
RX PubMed=20351219; DOI=10.1534/genetics.110.114066;
RA Lu Q., Yan J., Adler P.N.;
RT "The Drosophila planar polarity proteins inturned and multiple wing hairs
RT interact physically and function together.";
RL Genetics 185:549-558(2010).
CC -!- FUNCTION: Plays a role in the definition of cell polarity via the
CC planar cell polarity (PCP) cascade. Acts downstream of fuz and
CC accumulates asymmetrically in wing cells to regulate planar polarity in
CC the wing. Probably acts by regulating ciliogenesis.
CC {ECO:0000269|PubMed:11973308, ECO:0000269|PubMed:18701542,
CC ECO:0000269|PubMed:20351219, ECO:0000269|PubMed:8631273}.
CC -!- SUBUNIT: Interacts with mwh. {ECO:0000269|PubMed:20351219}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10570461}. Cell
CC membrane {ECO:0000269|PubMed:10570461}. Note=Localizes in the vicinity
CC of plasma membrane and the cortical actin cytoskeleton of wing disk and
CC pupal wing cells (PubMed:10570461). Localizes to the proximal side of
CC wing cells, fuz and frtz are required for its localization
CC (PubMed:15556868).
CC -!- DISRUPTION PHENOTYPE: Abnormal wing hair polarity and in many wing
CC cells forming two or more hairs instead of the normal single hair.
CC {ECO:0000269|PubMed:8631273}.
CC -!- SIMILARITY: Belongs to the inturned family. {ECO:0000305}.
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DR EMBL; U37134; AAC47004.1; -; mRNA.
DR EMBL; AE014296; AAF51582.2; -; Genomic_DNA.
DR EMBL; AY089567; AAL90305.1; -; mRNA.
DR RefSeq; NP_788548.1; NM_176370.3.
DR AlphaFoldDB; Q9VPH0; -.
DR BioGRID; 65506; 12.
DR IntAct; Q9VPH0; 3.
DR STRING; 7227.FBpp0077841; -.
DR PaxDb; Q9VPH0; -.
DR EnsemblMetazoa; FBtr0078183; FBpp0077841; FBgn0001259.
DR GeneID; 40246; -.
DR KEGG; dme:Dmel_CG16993; -.
DR UCSC; CG16993-RA; d. melanogaster.
DR CTD; 40246; -.
DR FlyBase; FBgn0001259; in.
DR VEuPathDB; VectorBase:FBgn0001259; -.
DR eggNOG; ENOG502QQJQ; Eukaryota.
DR GeneTree; ENSGT00390000001301; -.
DR HOGENOM; CLU_014223_0_0_1; -.
DR InParanoid; Q9VPH0; -.
DR OMA; WKEINNV; -.
DR OrthoDB; 898612at2759; -.
DR PhylomeDB; Q9VPH0; -.
DR Reactome; R-DME-450728; Inhibition of actin polymerisation.
DR Reactome; R-DME-5610787; Hedgehog 'off' state.
DR BioGRID-ORCS; 40246; 0 hits in 1 CRISPR screen.
DR ChiTaRS; if; fly.
DR GenomeRNAi; 40246; -.
DR PRO; PR:Q9VPH0; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0001259; Expressed in capitellum (Drosophila) and 27 other tissues.
DR Genevisible; Q9VPH0; DM.
DR GO; GO:0005929; C:cilium; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:FlyBase.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0001737; P:establishment of imaginal disc-derived wing hair orientation; TAS:FlyBase.
DR GO; GO:0007399; P:nervous system development; IBA:GO_Central.
DR GO; GO:0035316; P:non-sensory hair organization; IMP:FlyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR043987; CCZ1/INTU/HSP4_longin_1.
DR InterPro; IPR043989; CCZ1/INTU/HSP4_longin_3.
DR InterPro; IPR043988; CCZ1/INTU_longin_2.
DR InterPro; IPR039151; INTU.
DR InterPro; IPR036034; PDZ_sf.
DR PANTHER; PTHR21082; PTHR21082; 1.
DR Pfam; PF19031; Intu_longin_1; 1.
DR Pfam; PF19032; Intu_longin_2; 1.
DR Pfam; PF19033; Intu_longin_3; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cilium biogenesis/degradation; Cytoplasm;
KW Developmental protein; Membrane; Reference proteome.
FT CHAIN 1..869
FT /note="Protein inturned"
FT /id="PRO_0000416287"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 170
FT /note="V -> L (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="K -> Q (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
FT CONFLICT 231
FT /note="R -> Q (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="R -> G (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
FT CONFLICT 440..442
FT /note="QLR -> HVP (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
FT CONFLICT 803
FT /note="V -> I (in Ref. 1; AAC47004)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 869 AA; 98133 MW; 84B5393FF4A7F21E CRC64;
MRKSPASLPS EAFSSSNSSL SNSYSDGSDL ANWEEYVSTD GSLFYMEYVR CEKTGVSAER
RVEFMRRSLR LGKKSSRRQP HSNNQNQSQN QNQSHGPSRL DSQTEPQEFR FSQFKTAAPD
PKKLDLVITA ADRFRFGRRS TAVESILGFR VLPFPDQPEC LMVDGFVHDV SALQHGIKRG
DWFRSLNGIE VYASNVDELL KQFVEPTQVC LGFQSCGAAS AVSPTDPGYN RNVREEQVCK
VENFPMFTEK FEQMLITEGN FGIPGSSVDI RMPFAMLLLP PECYQHEQQQ DSLYYFPEIP
DNFLFKARGS FLTLHAVLSE LHTQPLSSRL LVDQVQYHVN YRQLNGFLVL FAYAGGLCCA
AECSLRSDEL IGYIRFSLPG LVLESFNQER EPGNSSRLKL FLRHFCEIQR TRLVARCHGH
IRFEELLGQS RSLPLPKEAQ LRIFDALSEM EAMDYRNWND EPLTTHREFF IYGSALYYDG
FLVASLLPPE VRVSVEGFLR CRGIFELLGA APGIKVREMY VWEEIVLPSA TGRYFLTICT
KNHLSLAVIL KIFDAPDMAP DAVVGPSLFY IEEIQETLDH LVQCGIESLA MFWSVSNKRP
EVLDATASES RDQEKEPNNR LESFLKQKLT VLSPSVEEEA QLCSSLGGSS IHSLTPSEDE
SCRRRLTPIH GTAEDSDSGS DWENFAVQHP LHYGLNLGAE SHSQSQMTES MWKEINNVVP
VKISAGWKNS VLHYVYIDIA NGSLFAPFTD SSENSNYLSE IRQACHIIHA VLQKSKHYRR
HLSESSRAQT NSNGHSSHTV SLVKEHGMIL EVSTQPKSDG QSQSSTSSRF VVVGRLFQSP
AKEVYVCHRS DVPQNIVEMA FRLSFFSMG
