ID   INT_BACSU               Reviewed;         368 AA.
AC   P96629; Q797K1;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=ICEBs1 integrase;
GN   Name=int; Synonyms=ydcL; OrderedLocusNames=BSU04800;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=18005101; DOI=10.1111/j.1365-2958.2007.06000.x;
RA   Lee C.A., Auchtung J.M., Monson R.E., Grossman A.D.;
RT   "Identification and characterization of int (integrase), xis (excisionase)
RT   and chromosomal attachment sites of the integrative and conjugative element
RT   ICEBs1 of Bacillus subtilis.";
RL   Mol. Microbiol. 66:1356-1369(2007).
CC   -!- FUNCTION: Putative integrase that is involved in the insertion of the
CC       integrative and conjugative element ICEBs1. Required for the excision
CC       of ICEBs1 from the donor cell genome and subsequent integration in the
CC       recipient cell genome. Appears not to be transferred through the mating
CC       pore. Integration of ICEBs1 involves an attachment site in the
CC       chromosome, attB, and a site in the circular form of ICEBs1, attICEBs1.
CC       {ECO:0000269|PubMed:18005101}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; AB001488; BAA19318.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12287.1; -; Genomic_DNA.
DR   PIR; A69774; A69774.
DR   RefSeq; NP_388361.1; NC_000964.3.
DR   RefSeq; WP_009966615.1; NZ_JNCM01000031.1.
DR   AlphaFoldDB; P96629; -.
DR   SMR; P96629; -.
DR   STRING; 224308.BSU04800; -.
DR   TCDB; 3.A.7.14.3; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR   PaxDb; P96629; -.
DR   PRIDE; P96629; -.
DR   EnsemblBacteria; CAB12287; CAB12287; BSU_04800.
DR   GeneID; 938151; -.
DR   KEGG; bsu:BSU04800; -.
DR   PATRIC; fig|224308.179.peg.510; -.
DR   eggNOG; COG0582; Bacteria.
DR   InParanoid; P96629; -.
DR   OMA; TINKIMI; -.
DR   PhylomeDB; P96629; -.
DR   BioCyc; BSUB:BSU04800-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF14659; Phage_int_SAM_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..368
FT                   /note="ICEBs1 integrase"
FT                   /id="PRO_0000360640"
FT   DOMAIN          61..143
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          164..362
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        201
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        239
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        313
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        316
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        339
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        349
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   368 AA;  43480 MW;  2E9411C9AB69BAAE CRC64;
     MSVKKLENGQ YQVDVSFGFD PITGERIRTR PVASTRKEAL ELEAKLRREF QEERARKSRS
     VSFPTLISIY LASCEIDSKP NYYQNQKYII NKHISDYFLK SDIQKITHRE ITDFRKHLME
     TGLSNKSVNN IMTSLSKIFD TAVHEEILKR NPCDNVKRLP LTRKKMKFWR PEEFKKFISL
     IPQDQLLFKT FYTVAFLTGL RCGEMLALQW KDIDKILLEI DVHKSCTWLD GQFVVTTPKT
     KNSIRRVSIN KKLLKLLERW KEAQEELFNE LGIRHSHDTY IFQYKDTPSR KDIFSRKIKY
     FCKDSDLTPI RLHDFRHSHV ALLIHQGEDY ITIKERLGHG SVKTTIDVYG HLYPNKQKEM
     ADKLDDLL