INV1_ORYSI
ID INV1_ORYSI Reviewed; 577 AA.
AC A2X5P7; Q6EU76; Q6VEF4; Q8S922;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1;
DE EC=3.2.1.26;
DE AltName: Full=Cell wall beta-fructosidase 1;
DE AltName: Full=Invertase 1;
DE AltName: Full=OsCIN1;
DE AltName: Full=Sucrose hydrolase 1;
DE Flags: Precursor;
GN Name=CIN1; ORFNames=OsI_007390;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Minghui 86;
RA Wang Y.-Q., Zhu Z.;
RT "Characterization of three rice cell wall invertase genes.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: May play a role in sucrose partitioning during seed
CC development and in stress response. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC the cell wall. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
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DR EMBL; AY342319; AAQ24869.1; -; mRNA.
DR EMBL; CM000127; EAY86157.1; -; Genomic_DNA.
DR AlphaFoldDB; A2X5P7; -.
DR SMR; A2X5P7; -.
DR STRING; 39946.A2X5P7; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR HOGENOM; CLU_001528_6_0_1; -.
DR BRENDA; 3.2.1.26; 4460.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.115.10.20; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF75005; SSF75005; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Cell wall; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..577
FT /note="Beta-fructofuranosidase, insoluble isoenzyme 1"
FT /id="PRO_0000303010"
FT ACT_SITE 63
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 333
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 212
FT /note="R -> L (in Ref. 1; AAQ24869)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="K -> T (in Ref. 1; AAQ24869)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="S -> F (in Ref. 1; AAQ24869)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 577 AA; 63994 MW; 6441FA23D4E3170E CRC64;
MGTRLLALAP WLLLLLLQLA GASHVVHRSL EAEQAPSSVP ASIVSPLLRT GYHFQPPMNW
INDPNGPLYY KGWYHLFYQY NPKGAVWGNI VWAHSVSQDL INWIALEPAI KPDIPSDQYG
CWSGSATILP DGTPAILYTG IDRPNINYQV QNIAFPKNAS DPLLREWVKP AYNPVATPEP
GMNATQFRDP TTAWYADGHW RMLVGGLKGA RRGLAYLYRS RDFKTWVRAK HPLHSALTGM
WECPDFFPLQ APGLQAGLDT SVPSSKYVLK NSLDLTRYDY YTVGIYNKVT ERYVPDNPAG
DYHRLRYDYG NFYASKTFFD PVKHRRILLG WANESDSVTY DKAKGWAGIH AIPRKVWLDP
SGKQLLQWPI EELEKLRGKS VSVSDKVVKP GEHFQVTGLG TYQADVEVSL EVSGLEKAEA
LDPAFGDDAE RLCGAKGADV RGGVVFGLWV LASAGLEEKT AVFFRVFKPA GHGAKPVVLM
CTDPTKSSLS PDLYKPTFAG FVDTDISSGK ISLRSLIDRS VVESFGAGGK TCILSRVYPS
MAIGDKAHLY VFNNGEADIK ISHLKAWEMK KPLMNGA
