INV3_ORYSI
ID INV3_ORYSI Reviewed; 586 AA.
AC Q01IS8; A2XT81; Q56UD3; Q6VEF3; Q7XVJ3; Q84TL3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 3;
DE EC=3.2.1.26;
DE AltName: Full=Cell wall beta-fructosidase 3;
DE AltName: Full=Invertase 3;
DE AltName: Full=OsCIN3;
DE AltName: Full=Sucrose hydrolase 3;
DE Flags: Precursor;
GN Name=CIN3; Synonyms=INV4; ORFNames=OsI_015274, OSIGBa0134P10.8;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. IR36;
RA Oliver S.N., Fernandes S.Q., Dennis E.S., Dolferus R.;
RT "Identification of four invertase genes with expression in the rice
RT anther.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Minghui 86;
RA Wang Y.-Q., Zhu Z.;
RT "Characterization of three rice cell wall invertase genes.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Guang-Lu-Ai No.4;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC the cell wall. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAH67362.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY220486; AAO63553.1; -; Genomic_DNA.
DR EMBL; AY342320; AAQ24870.1; -; mRNA.
DR EMBL; CR855194; CAH67362.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q01IS8; -.
DR SMR; Q01IS8; -.
DR STRING; 39946.Q01IS8; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR EnsemblPlants; BGIOSGA016363-TA; BGIOSGA016363-PA; BGIOSGA016363.
DR Gramene; BGIOSGA016363-TA; BGIOSGA016363-PA; BGIOSGA016363.
DR HOGENOM; CLU_001528_6_0_1; -.
DR OMA; TPKMNAP; -.
DR BRENDA; 3.2.1.26; 4460.
DR Proteomes; UP000007015; Chromosome 4.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.115.10.20; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF75005; SSF75005; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Cell wall; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..586
FT /note="Beta-fructofuranosidase, insoluble isoenzyme 3"
FT /id="PRO_0000303012"
FT ACT_SITE 61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT CARBOHYD 154
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 179
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 341
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 390
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 479
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 89
FT /note="E -> K (in Ref. 2; AAQ24870)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="P -> L (in Ref. 2; AAQ24870)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="Y -> H (in Ref. 2; AAQ24870)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="I -> V (in Ref. 2; AAQ24870)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="Q -> R (in Ref. 2; AAQ24870)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 586 AA; 66208 MW; C7D262407F51245F CRC64;
MATARARAAL VFVALLQMAA VVVVRASHVV YPELQSLEAK HVDGKLRTGY HFQPPKHWIN
DPNGPMYYKG LYHLFYQYNP KGAVWGNIEW AHSVSTDLID WTALEPGIYP SKTFDEKGCW
SGSATVLPSG VPVIMYTGID PDERQVQNVA YPVNLSDPYL REWYKPDYNP IINPDGGINA
SAFRDPTTAW YGPDGHWRLL VGSKVNMKGL AVLYRSRDFK KWVKAHHPLH SAHTGMWECP
DFFPVAVAGG SRHYRRGVDT AELHDAAVAE EVKYVLKVSL DLTRYEYYTV GWYDHATDRY
VPDAAFPDND YGLRYDYGDF YASKSFYDPA KRRRIVWGWA NESDTVPDDR RKGWAGIQAI
PRKLWLSADG KQLVQWPVEE LKALRAKHVN VTDKVIKKGN YFEVTGFKSV QSDVDMAFAI
KDLSKAEEFD PAWRTDAEAL CKKLGSDVDG GVGPFGLWAL ASGDLKERTA VFFRVFKAND
SSHVVLMCND PTRSSYESKI YRPTFAGFVD VDIAKNKQIA LRTLIDHSVV ESFGARGKTC
ILTRVYPRKA VGDDAHLFVF NNGESDVKVT NLDAWEMKTP KMNAEE
