ID   INV3_ORYSJ              Reviewed;         586 AA.
AC   Q0JDC6; A3ATP1; B9FF34; Q56UD3; Q6VEF3; Q7XVJ3; Q84TL3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 3;
DE            EC=3.2.1.26;
DE   AltName: Full=Cell wall beta-fructosidase 3;
DE   AltName: Full=Invertase 3;
DE   AltName: Full=OsCIN3;
DE   AltName: Full=Sucrose hydrolase 3;
DE   Flags: Precursor;
GN   Name=CIN3; Synonyms=INV4; OrderedLocusNames=Os04g0413200, LOC_Os04g33720;
GN   ORFNames=OJ000126_13.7, OsJ_014163,
GN   OsJ_14738 {ECO:0000312|EMBL:EEE60967.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15759120; DOI=10.1007/s00299-004-0910-z;
RA   Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H.,
RA   Lee K.-W., An G., Hahn T.-R., Jeon J.-S.;
RT   "Molecular cloning and expression analysis of the cell-wall invertase gene
RT   family in rice (Oryza sativa L.).";
RL   Plant Cell Rep. 24:225-236(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC       the cell wall. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Weakly expressed in flowers.
CC       {ECO:0000269|PubMed:15759120}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD40590.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY578160; AAT84403.1; -; mRNA.
DR   EMBL; AL662945; CAD40590.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008210; BAF14661.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS89134.1; -; Genomic_DNA.
DR   EMBL; CM000141; EEE60967.1; -; Genomic_DNA.
DR   RefSeq; XP_015635053.1; XM_015779567.1.
DR   AlphaFoldDB; Q0JDC6; -.
DR   SMR; Q0JDC6; -.
DR   STRING; 4530.OS04T0413200-01; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   PaxDb; Q0JDC6; -.
DR   PRIDE; Q0JDC6; -.
DR   EnsemblPlants; Os04t0413200-01; Os04t0413200-01; Os04g0413200.
DR   GeneID; 4335789; -.
DR   Gramene; Os04t0413200-01; Os04t0413200-01; Os04g0413200.
DR   KEGG; osa:4335789; -.
DR   eggNOG; KOG0228; Eukaryota.
DR   HOGENOM; CLU_001528_6_0_1; -.
DR   InParanoid; Q0JDC6; -.
DR   OMA; TPKMNAP; -.
DR   OrthoDB; 405663at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000007752; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q0JDC6; OS.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR018053; Glyco_hydro_32_AS.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
DR   PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Cell wall; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..586
FT                   /note="Beta-fructofuranosidase, insoluble isoenzyme 3"
FT                   /id="PRO_0000033381"
FT   ACT_SITE        61
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT   BINDING         58..61
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         77
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         85
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         120..121
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         184..185
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         238
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         281
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        154
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        179
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        341
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        390
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        479
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        441..488
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   586 AA;  66208 MW;  C7D262407F51245F CRC64;
     MATARARAAL VFVALLQMAA VVVVRASHVV YPELQSLEAK HVDGKLRTGY HFQPPKHWIN
     DPNGPMYYKG LYHLFYQYNP KGAVWGNIEW AHSVSTDLID WTALEPGIYP SKTFDEKGCW
     SGSATVLPSG VPVIMYTGID PDERQVQNVA YPVNLSDPYL REWYKPDYNP IINPDGGINA
     SAFRDPTTAW YGPDGHWRLL VGSKVNMKGL AVLYRSRDFK KWVKAHHPLH SAHTGMWECP
     DFFPVAVAGG SRHYRRGVDT AELHDAAVAE EVKYVLKVSL DLTRYEYYTV GWYDHATDRY
     VPDAAFPDND YGLRYDYGDF YASKSFYDPA KRRRIVWGWA NESDTVPDDR RKGWAGIQAI
     PRKLWLSADG KQLVQWPVEE LKALRAKHVN VTDKVIKKGN YFEVTGFKSV QSDVDMAFAI
     KDLSKAEEFD PAWRTDAEAL CKKLGSDVDG GVGPFGLWAL ASGDLKERTA VFFRVFKAND
     SSHVVLMCND PTRSSYESKI YRPTFAGFVD VDIAKNKQIA LRTLIDHSVV ESFGARGKTC
     ILTRVYPRKA VGDDAHLFVF NNGESDVKVT NLDAWEMKTP KMNAEE