ID   INV3_YEASX              Reviewed;          74 AA.
AC   P10595;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Invertase 3;
DE            EC=3.2.1.26;
DE   AltName: Full=Beta-fructofuranosidase 3;
DE   AltName: Full=Saccharase;
DE   Flags: Precursor; Fragment;
GN   Name=SUC3;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2835632; DOI=10.1007/bf00425699;
RA   Hohmann S., Gozalbo D.;
RT   "Structural analysis of the 5' regions of yeast SUC genes revealed
RT   analogous palindromes in SUC, MAL and GAL.";
RL   Mol. Gen. Genet. 211:446-454(1988).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
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DR   EMBL; X07571; CAA30458.1; -; Genomic_DNA.
DR   PIR; S25439; S25439.
DR   AlphaFoldDB; P10595; -.
DR   SMR; P10595; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   SGD; S000029532; SUC3.
DR   VEuPathDB; FungiDB:YIL162W; -.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR018053; Glyco_hydro_32_AS.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
DR   PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..19
FT   CHAIN           20..>74
FT                   /note="Invertase 3"
FT                   /id="PRO_0000033401"
FT   ACT_SITE        42
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT   BINDING         39..42
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         60
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        23
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        64
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         74
SQ   SEQUENCE   74 AA;  8650 MW;  D93740931C62EEF2 CRC64;
     MLLQAFIFLL AGFAAKISAL MTNETSDRPL VHFTPNKGWM NDPNGLWYDA KEGKWHLYFQ
     YNPNDTVWGL PLFW