INV4_ORYSJ
ID INV4_ORYSJ Reviewed; 590 AA.
AC Q5JJV0; Q0JFR0; Q75SP0;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 4;
DE EC=3.2.1.26;
DE AltName: Full=Cell wall beta-fructosidase 4;
DE AltName: Full=Invertase 4;
DE AltName: Full=OsCIN4;
DE AltName: Full=Sucrose hydrolase 4;
DE Flags: Precursor;
GN Name=CIN4; OrderedLocusNames=Os01g0966700, LOC_Os01g73580;
GN ORFNames=OsJ_04889 {ECO:0000312|EMBL:EEE56070.1}, P0458E05.32;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=15759120; DOI=10.1007/s00299-004-0910-z;
RA Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H.,
RA Lee K.-W., An G., Hahn T.-R., Jeon J.-S.;
RT "Molecular cloning and expression analysis of the cell-wall invertase gene
RT family in rice (Oryza sativa L.).";
RL Plant Cell Rep. 24:225-236(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Panicle;
RA Hirose T., Terao T.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=15701658; DOI=10.1093/pcp/pci066;
RA Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H.,
RA Terao T., Ishii R., Ohsugi R., Yamagishi T.;
RT "Expression patterns of genes encoding carbohydrate-metabolizing enzymes
RT and their relationship to grain filling in rice (Oryza sativa L.):
RT comparison of caryopses located at different positions in a panicle.";
RL Plant Cell Physiol. 46:620-628(2005).
CC -!- FUNCTION: May play a role in sucrose partitioning during seed
CC development and in stress response.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC the cell wall. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves. Expressed at moderate levels
CC in roots and flowers, and weakly in seeds.
CC {ECO:0000269|PubMed:15759120}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 1 to 10 days after flowering.
CC {ECO:0000269|PubMed:15701658, ECO:0000269|PubMed:15759120}.
CC -!- INDUCTION: By rice blast fungus (M.grisea) 12 hours after infection.
CC {ECO:0000269|PubMed:15759120}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY578161; AAT84404.1; -; mRNA.
DR EMBL; AB154521; BAD05180.1; -; mRNA.
DR EMBL; AP004365; BAD88258.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF07418.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS76386.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE56070.1; -; Genomic_DNA.
DR EMBL; AK067444; BAG90423.1; -; mRNA.
DR RefSeq; XP_015622113.1; XM_015766627.1.
DR AlphaFoldDB; Q5JJV0; -.
DR SMR; Q5JJV0; -.
DR STRING; 4530.OS01T0966700-01; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR PaxDb; Q5JJV0; -.
DR PRIDE; Q5JJV0; -.
DR EnsemblPlants; Os01t0966700-01; Os01t0966700-01; Os01g0966700.
DR GeneID; 4324001; -.
DR Gramene; Os01t0966700-01; Os01t0966700-01; Os01g0966700.
DR KEGG; osa:4324001; -.
DR eggNOG; KOG0228; Eukaryota.
DR HOGENOM; CLU_001528_6_0_1; -.
DR InParanoid; Q5JJV0; -.
DR OMA; GGRACIM; -.
DR OrthoDB; 405663at2759; -.
DR PlantReactome; R-OSA-1119626; Fructan degradation.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5JJV0; baseline and differential.
DR Genevisible; Q5JJV0; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.115.10.20; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF75005; SSF75005; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Cell wall; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..590
FT /note="Beta-fructofuranosidase, insoluble isoenzyme 4"
FT /id="PRO_0000033382"
FT ACT_SITE 74
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT BINDING 71..74
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 98
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 133..134
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 198..199
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 253
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 287
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 94
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 247
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 345
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 565
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 445..491
FT /evidence="ECO:0000250"
FT CONFLICT 226
FT /note="L -> P (in Ref. 2; BAD05180)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 66610 MW; 4999F60849A679E4 CRC64;
MVMAPIPQPW HQWPFLILFF LVLFSCESNL PCRNGVEATQ RVFLYPQSPK VSSIVSKGYR
TGYHFQPPKN WINDPNGPMY YNGIYHEFYQ YNPNGSVWGN IVWGHSVSTD LINWIRLEPA
IEGNTPSDIN GCWTGSATIL TGDQPVIIYT GADTEKRQVQ NIVLPKNRSD PYLREWTKPK
NNPLIEPVGP GLNSNQFRDP TTGWIGPDGL WRIAVGAELN GYSAALLYKS KDFMQWTRVD
HPLYSSNASN MWECPDFFAV LPGKNNGLDL SAAIPNGAKH VLKMSLDSCD KYMIGVYDLK
HDMFVPDTVL DDRRLWLRID YGNYYASKSF FDSKKGRRII WGWTNETDST SDDVAKGWAG
IHAIPRTIWL DGDGKRLLQW PIEEVESLRR NEVSHQGLEL KKGDLFEIKG TDTLQADVEI
DFELTSIDAA DPFDPSWLLD TEKHCREADA SVHGGLGPFG LVVLASDNMD EHTTVHFRVY
KSEQKYMVLL CSDLRRSSLR PGLYTPAYGG FFEYDLEKEK KISLRTLIDR SAVESFGGGG
RACIMARVYP AAVVDGATHM YAFNNGSSTV KVSQLKAWSM TRAQVNVRKG
